Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx

... Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli Wael Ismail 1 , Magdy El-Said Mohamed 1 , Barry L. ... (260 nm) and by online liquid- scintillation counting. The retention times for phenylacetate, phenylacetyl-CoA and 2-hydroxyphenylacetate were 20, 21 and 12 min, respectively. NMR spectroscopy The ... labeled phenylacetate in...

Ngày tải lên: 23/03/2014, 18:20

... DNA binding domain and VP16 activation domain. Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig. 1A) and tested in ... reporter genes in cells. An EcR gene switch that includes a fusion protein containing VP16 activation domain, GAL4 DNA binding domain and EcR DEF domains and a chimera containing helices 1–8 .....

Ngày tải lên: 16/03/2014, 12:20

... Biology, Aarhus University, Denmark Introduction Translation initiation factor 1 (IF1), encoded by infA, is a small protein consisting of 71 amino acids in Escherichia coli. It is essential for cell ... fMet-tRNA binding to the P-site [10,12,13], probably by stabilizing IF2 binding to the 30S subunit [14], and stimulates the GTPase activity of IF2 [15]. It also increases binding of m...

Ngày tải lên: 29/03/2014, 09:20

... are indicated by arrows. The N-terminal tail (residues 1–61) of the protein is coloured in orange, the large domain (residues 62– 211) in salmon, the interdomain segment (residues 212–279) in green ... was investigated by performing equilib- rium unfolding experiments, using urea as denaturing agent. The structural changes of the active site and overall protein induced by urea wer...

Ngày tải lên: 30/03/2014, 02:20

... dimension. A sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr and 2D winnmr software ... NMR signals are not specifically bound to the induction of apoptosis, in agreement with recent findings [8]. Second, the intensity of the ML signal, correspond- ing to TG levels, is increased...

Ngày tải lên: 18/02/2014, 13:20

... origin [3,6–12]. These proteins, such as BamA (formerly YaeT) in Escherichia coli, Toc75 and Oep80 in chloroplasts, and Sam50 in mitochondria, are essential parts of complexes involved in protein ... encoding a secretion-competent, N-terminal FHA derivative called Fha44 in E. coli [31], and the secretion of Fha44 in culture supernatants was determined by semiquantita- tive...

Ngày tải lên: 23/03/2014, 03:20

... Fumarate-binding site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate- binding site of m-NAD-ME. The corresponding amino acids in the fumarate-binding ... both being in dimer form in low protein concentrations and being reconstituted into tetramers in high protein concentrations. In contrast to the E59Q enzyme, the E59D enzyme has a...

Ngày tải lên: 23/03/2014, 06:20

... propionyl-CoA to succinyl-CoA. The Caenor- habditis elegans genome contains a single gene encoding MCE (mce-1) cor- responding to a 15 kDa protein. This was expressed in Escherichia coli and the enzymatic ... mm MgCl 2 ,75lm methylmalonyl-CoA. After the initial 5 min incubation, purified MCE was added and incubation was continued for an additional 1–5 min. Reactions were terminated by...

Ngày tải lên: 23/03/2014, 13:20

... protein folding intermediates [11–18], there are no reports available for E. coli invasin pro- tein IbeA. The process of unfolding and refolding is useful for a complex unfolding transition, indicating their ... buffer containing 100 mm SDS and either boiled for 5 min or directly loaded onto the gel. In all experiments, 4–20% gels were used. Protein was detected by staining with Safe Co...

Ngày tải lên: 07/03/2014, 05:20

... quantities of protein utiliz- ing the principle of protein-dye binding. Anal Biochem 72, 248–254. 40 Gatlin CL, Kleemann GR, Hays LG, Link AJ & Yates JR III (1998) Protein identification at ... homology with domains I and II of EF-P, binds to certain mRNAs in a hypusine-dependent manner [31]. Our MS analysis of EF-P indicates that Lys34 of the protein is modified by spermidine, which is...

Ngày tải lên: 16/03/2014, 06:20