Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
... Functional genomics by NMR spectroscopy
Phenylacetate catabolism in
Escherichia coli
Wael Ismail
1
, Magdy El-Said Mohamed
1
, Barry L. ... (260 nm) and by online liquid-
scintillation counting. The retention times for phenylacetate,
phenylacetyl-CoA and 2-hydroxyphenylacetate were 20, 21
and 12 min, respectively.
NMR spectroscopy
The ... labeled phenylacetate in...
Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx
... DNA binding domain and VP16
activation domain. Various combinations of the EcR
ligand binding domain, VP16 activation domain and
GAL4 DNA binding domain were constructed
(Fig. 1A) and tested in ... reporter
genes in cells. An EcR gene switch that includes a fusion
protein containing VP16 activation domain, GAL4
DNA binding domain and EcR DEF domains and a
chimera containing helices 1–8 .....
Báo cáo khoa học: Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin pdf
... Biology, Aarhus University, Denmark
Introduction
Translation initiation factor 1 (IF1), encoded by infA,
is a small protein consisting of 71 amino acids in
Escherichia coli. It is essential for cell ... fMet-tRNA binding to the P-site
[10,12,13], probably by stabilizing IF2 binding to the
30S subunit [14], and stimulates the GTPase activity of
IF2 [15]. It also increases binding of m...
Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx
... are indicated by arrows. The N-terminal tail (residues 1–61)
of the protein is coloured in orange, the large domain (residues 62–
211) in salmon, the interdomain segment (residues 212–279) in
green ... was investigated by performing equilib-
rium unfolding experiments, using urea as denaturing
agent. The structural changes of the active site and
overall protein induced by urea wer...
Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc
... dimension. A sine function was
applied in both dimensions of the time domain before
Fourier transformation.
Integration of 1D and 2D signals was performed using
1D winnmr and 2D winnmr software ... NMR signals are not specifically bound to
the induction of apoptosis, in agreement with recent
findings [8].
Second, the intensity of the ML signal, correspond-
ing to TG levels, is increased...
Báo cáo khoa học: Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily ppt
... origin [3,6–12]. These
proteins, such as BamA (formerly YaeT) in Escherichia
coli, Toc75 and Oep80 in chloroplasts, and Sam50 in
mitochondria, are essential parts of complexes involved
in protein ... encoding a
secretion-competent, N-terminal FHA derivative called
Fha44 in E. coli [31], and the secretion of Fha44 in
culture supernatants was determined by semiquantita-
tive...
Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... Fumarate-binding site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate-
binding site of m-NAD-ME. The corresponding amino acids in the fumarate-binding ... both
being in dimer form in low protein concentrations and
being reconstituted into tetramers in high protein
concentrations. In contrast to the E59Q enzyme, the
E59D enzyme has a...
Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx
... propionyl-CoA to succinyl-CoA. The Caenor-
habditis elegans genome contains a single gene encoding MCE (mce-1) cor-
responding to a 15 kDa protein. This was expressed in Escherichia coli and
the enzymatic ... mm
MgCl
2
,75lm methylmalonyl-CoA. After the initial 5 min
incubation, purified MCE was added and incubation was
continued for an additional 1–5 min. Reactions were
terminated by...
Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf
... protein folding intermediates [11–18],
there are no reports available for E. coli invasin pro-
tein IbeA. The process of unfolding and refolding is
useful for a complex unfolding transition, indicating
their ... buffer
containing 100 mm SDS and either boiled for 5 min or
directly loaded onto the gel. In all experiments, 4–20% gels
were used. Protein was detected by staining with Safe Co...
Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc
... quantities of protein utiliz-
ing the principle of protein-dye binding. Anal Biochem
72, 248–254.
40 Gatlin CL, Kleemann GR, Hays LG, Link AJ & Yates
JR III (1998) Protein identification at ... homology with
domains I and II of EF-P, binds to certain mRNAs in
a hypusine-dependent manner [31]. Our MS analysis of
EF-P indicates that Lys34 of the protein is modified
by spermidine, which is...