... of this signaling pathwayalso enhances metabolism, resulting in an increase in glucose uptake and an up-regulation of glycolysis[37,38]. In addition, PI3K signaling has been shown tobe involved ... nonfat milk in NaCl ⁄ Tris containing 0.2% NonidetP40, incubated with primary antibodies overnight at 4 °Cand washed in NaCl ⁄ Tris–Tween (0.1% Tween-20). Immu-noblotting was detected by enhanced ... placed into scintillation vials containing scin-tillation liquid (ScintiSafe Plus 50%, Fisher ScientificCanada, Ottawa, Canada). Radioactivity was counted 24 hlater, using a liquid scintillation...