Báo cáo khoa học: Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding docx

... human cytochrome P450 reductase (Eur. J. Biochem. 270) 2621 Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding Relaxation kinetic studies with coenzyme ... bound to the enzyme predominantly through inter- actions involving its adenine end, whose binding site is contained within the FAD -binding domain of the...

Ngày tải lên: 23/03/2014, 17:22

... kinetics of cytochrome P450 reductase: internal electron transfer is limited by confor- mational change and regulated by coenzyme binding. Biochemistry 41, 4626–4637. 35 Marcus RA & Sutin ... in the mechanism of electron transfer in this complex flavoprotein. Abbreviations CPR, cytochrome P450 reductase; di-sq, di-semiquinone; ET, electron transfer; hq, h...

Ngày tải lên: 07/03/2014, 06:20

... oleovorans rubredoxin reductase- rubredoxin electron transfer com- plex. Biochemistry 37, 15513–15522. 23 Ghisla S & Edmondson DA (2001) Flavin Coenzymes. In Encyclopedia of Life Sciences. Nature Publishing Group. ... NO, FlRd has to be reduced by NADH via the FAD -binding protein flavorubredoxin reduc- tase, thus the kinetics of electron transfer along this pathway was inves...

Ngày tải lên: 16/03/2014, 11:20

... ⁄ LEDGF-interacting domain linked to DNA -binding domains (AT-hook and MT domain) becomes disconnected from the PHD domains, the FYRN domain, the transactivating domain, the FYRC domain and the ... denominator in all different MLL rearrangements is disruption of the MLL protein in a region that prevents any subsequent pro- tein–protein interaction between the resulting MLL fusion prote...

Ngày tải lên: 16/02/2014, 14:20

... cyclin dependent kinase 4 is controlled by multiple mechanisms, including phosphorylation of tyrosine 17. This site is equivalent to tyrosine 15 of cyclin dependent kinase 1, which undergoes inhibitory ... that this phosphory- lation is independent of p27 KIP1 . The finding that Y17 phosphorylation is mediated by Src family kinases is completely novel and it is interesting to n...

Ngày tải lên: 18/02/2014, 18:20

... by unwinding [38] and are potential sites for aggregation of S ⁄ MAR -binding proteins. In this view, the direct association between DNA-PK and S ⁄ MAR-DNA or -binding proteins is a considerable point. ... analysis of the temporo-spatial distribution of DNA repair factors in situ in living cells has been providing us with striking information. For instance, even following exposur...

Ngày tải lên: 19/02/2014, 06:20

... of this signaling pathway also enhances metabolism, resulting in an increase in glucose uptake and an up-regulation of glycolysis [37,38]. In addition, PI3K signaling has been shown to be involved ... nonfat milk in NaCl ⁄ Tris containing 0.2% Nonidet P40, incubated with primary antibodies overnight at 4 °C and washed in NaCl ⁄ Tris–Tween (0.1% Tween-20). Immu- noblotting was detect...

Ngày tải lên: 07/03/2014, 00:20

... Site-directed mutational analysis for the ATP binding of DnaA pro- tein. Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP -binding domain of DnaA protein in vitro and in vivo. J Biol ... adenosine 5¢-(b,c-imido)triphosphate, indicating that in order to inhibit iron regulatory protein-1 binding activity, ATP must be hydrolyzed. Indeed, recombinant ir...

Ngày tải lên: 07/03/2014, 09:20

... dynamics. Biochemistry 35, 7692–7704. 19 Steinhoff HJ (2004) Inter- and intra-molecular distances determined by EPR spectroscopy and site-directed spin labeling reveal protein-protein and protein-oligonucleo- tide ... spin-labeling experiments could reveal where these interactions originate from, either by mutation of the possibly interacting residues or by using double site-directed sp...

Ngày tải lên: 16/03/2014, 01:20

... as shown by ELISA. A plateau noted for the increase in cystatin C at longer incuba- tion times is likely due to increased competition with cystatin C -binding proteins (i.e. target cysteine prote- ases) ... proteases is balanced by tight -binding inhibitors, the cystatins [4]. The cysta- tin protein family comprises three major groups of inhibitors: type 1 cystatins, also called s...

Ngày tải lên: 16/03/2014, 06:20