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Báo cáo khoa học: Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding docx

Báo cáo khoa học: Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding docx

Báo cáo khoa học: Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding docx

... human cytochrome P450 reductase (Eur. J. Biochem. 270) 2621 Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding Relaxation kinetic studies with coenzyme ... bound to the enzyme predominantly through inter-actions involving its adenine end, whose binding site is contained within the FAD -binding domain of the protein(Fig. 2). The electron density for ... limited by conformational change and regulated by coenzyme binding [28]. This rate is lower (10 s)1) for dithionite-reducedenzyme, indicating that nicotinamide coenzyme optimizes interflavin electron...
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Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx

Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx

... kinetics of cytochrome P450 reductase: internal electron transfer is limited by confor-mational change and regulated by coenzyme binding. Biochemistry 41, 4626–4637.35 Marcus RA & Sutin ... in the mechanism of electron transfer in this complex flavoprotein.AbbreviationsCPR, cytochrome P450 reductase; di-sq, di-semiquinone; ET, electron transfer; hq, hydroquinone; KIE, kinetic isotope ... determination of the flavin redoxpotentials in full-length enzyme and isolated flavin-bind-ing domains. Biochemistry 42, 3911–3920.57 Wolthers KR & Scrutton NS (2004) Electron transfer in human...
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Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf

Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf

... oleovoransrubredoxin reductase- rubredoxin electron transfer com-plex. Biochemistry 37, 15513–15522.23 Ghisla S & Edmondson DA (2001) Flavin Coenzymes. In Encyclopedia of Life Sciences. Nature PublishingGroup. ... NO, FlRd has to bereduced by NADH via the FAD -binding protein flavorubredoxin reduc-tase, thus the kinetics of electron transfer along this pathway was investi-gated by stopped-flow absorption ... coli FlRd is the terminal component of an elec-tron transport chain (Fig. 1) that involves NADH andflavorubredoxin reductase, a FAD -binding protein ofthe NAD(P)H:rubredoxin oxidoreductase family....
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... ⁄ LEDGF-interactingdomain linked to DNA -binding domains (AT-hook andMT domain) becomes disconnected from the PHDdomains, the FYRN domain, the transactivatingdomain, the FYRC domain and the ... denominator in alldifferent MLL rearrangements is disruption of the MLLprotein in a region that prevents any subsequent pro-tein–protein interaction between the resulting MLLfusion proteins. ... orthologues in various biological systems, is a ubiquitously expressedprotein involved in chromatin regulation. MLL expres-sion is initiated at very early stages of embryogenesis.The MLL protein is...
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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

... cyclin dependentkinase 4 is controlled by multiple mechanisms, including phosphorylationof tyrosine 17. This site is equivalent to tyrosine 15 of cyclin dependentkinase 1, which undergoes inhibitory ... that this phosphory-lation is independent of p27KIP1.The finding that Y17 phosphorylation is mediated by Src family kinases is completely novel and it is interesting to note how this fits in with ... Src family kinase inhibitor PP2 [20]. In comparison, we also assayed the inhibition of puri-fied recombinant kinases C-SRC, C-YES and p49WEE1 by PP2 using the 96-well plate format kinase assay.p49WEE1is...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

... by unwinding [38] and are potential sites for aggregationof S ⁄ MAR -binding proteins. In this view, the directassociation between DNA-PK and S ⁄ MAR-DNA or -binding proteins is a considerable point. ... analysis of the temporo-spatial distributionof DNA repair factors in situ in living cells has beenproviding us with striking information. For instance,even following exposure to ionizing radiation ... stability of the complex against salt is also enhanced by the presence of Ku. Interestingly, thecomplex formation was easily inhibited by ssDNA,including poly(dT) and hairpin-ended DNA, althoughthese...
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Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... of this signaling pathwayalso enhances metabolism, resulting in an increase in glucose uptake and an up-regulation of glycolysis[37,38]. In addition, PI3K signaling has been shown tobe involved ... nonfat milk in NaCl ⁄ Tris containing 0.2% NonidetP40, incubated with primary antibodies overnight at 4 °Cand washed in NaCl ⁄ Tris–Tween (0.1% Tween-20). Immu-noblotting was detected by enhanced ... placed into scintillation vials containing scin-tillation liquid (ScintiSafe Plus 50%, Fisher ScientificCanada, Ottawa, Canada). Radioactivity was counted 24 hlater, using a liquid scintillation...
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Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

... Site-directedmutational analysis for the ATP binding of DnaA pro-tein. Functions of two conserved amino acids (Lys-178and Asp-235) located in the ATP -binding domain ofDnaA protein in vitro and in vivo. J Biol ... adenosine 5¢-(b,c-imido)triphosphate, indicating that in order toinhibit iron regulatory protein-1 binding activity, ATP must be hydrolyzed.Indeed, recombinant iron regulatory protein-1 binds ... followed by UV-induced covalent crosslinking of bound [a-32P]nucleo-tide. The proteins were separated by SDS ⁄ PAGE, andABCFig. 3. ATP inhibits IRP–IRE binding activity. IRE binding activitywas...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx

Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx

... dynamics.Biochemistry 35, 7692–7704.19 Steinhoff HJ (2004) Inter- and intra-molecular distancesdetermined by EPR spectroscopy and site-directed spinlabeling reveal protein-protein and protein-oligonucleo-tide ... spin-labeling experiments could revealwhere these interactions originate from, either by mutation of the possibly interacting residues or by using double site-directed spin-labeling for distancemeasurements ... measuredusing pulsed double resonance EPR methods (e.g.pulsed electron- electron double resonance, double electron electron resonance) [19]. By combiningdistance and lineshape measurements, global informa-tion...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

... as shown by ELISA. A plateaunoted for the increase in cystatin C at longer incuba-tion times is likely due to increased competition withcystatin C -binding proteins (i.e. target cysteine prote-ases) ... proteases is balanced by tight -binding inhibitors, the cystatins [4]. The cysta-tin protein family comprises three major groups ofinhibitors: type 1 cystatins, also called stefins, whichare intracellular ... cyste-ine protease, legumain, because of removal of thekey amino acid in the legumain -binding site of cysta-tin C [22]. The amino acid substitutions reside in opposing parts of the cystatin C...
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