Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx
Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx
... 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis Dave Simard, Kerry A. Hewitt, Faylene Lunn, Akshai Iyengar and Stephen L. ... domain and wild-type ability to catalyse NH 3 -dependent CTP formation. For these mutants, the values of k cat /K m and k cat for glutamine-...
Ngày tải lên: 23/03/2014, 17:22
Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx
... (dFBA), and was applied for diauxic growth of E. coli on glucose and acetate [8]. The model predicts very well the time course of the external metabolites and the growth of biomass. In Santillan and ... system in Escherichia coli is a transport and sen- sory system and, in this function, is one of the key players of catabolite repression. Mathematical modelling o...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx
... biological molecules. Knowledge of the conformational properties of EspB may clarify the role of EspB in bacterial attachment, but no information about the structural properties of EspB is currently available. In ... less organ- ized conformations of EspB and IpaC may be a com- mon property for this class of proteins. Importantly, some proteins that are natively unfol- ded sho...
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... observed that both T-pIC and M-pIC treatments induced the phosphorylation at Ser386 and Ser396 of IRF-3, and formed the dimeriza- tion of IRF-3 (Fig. 5A,B, lanes 1 and 2), and that NS3-4A remarkably ... Grants-in-Aid for the Third-Term Comprehensive 10-Year Strategy for Cancer Control, and by a Grant-in-Aid for Research on Hepatitis, both from the Ministry of Health, L...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating fibrinolysis doc
... with (i) a mixture of A115 and F23, (ii) a mixture of A115 and F23, (iii) Y353 and (iv) A115. Table 5. Kinetic and stability parameters for purified WT and mutant CPUs. The T1 ⁄ 2 of activity at 37 ... in its name stands for unsta- ble [10,11]. The inherent and irreversible decay of CPU’s activity is believed to be of major importance for its in vivo down-regulation...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Endosomal proteolysis of diphtheria toxin without toxin translocation into the cytosol of rat liver in vivo pdf
... bond (Arg193–Ser194) and a disulfide bridge (Cys186– Cys201). Proteolytic nicking of the polypeptide and reduction of the disulfide are required for the A and B fragments to separate and for cytotoxicity ... mobilities of the immunoreactive proform ( 64 kDa) and mature form ( 45 kDa) of cathepsin D, and soluble trun- cated furin ( 70 kDa). (B) Eluted fractions (5, 7...
Ngày tải lên: 23/03/2014, 07:20
Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
... which aligns with His200 of the d 1 heme domain of cd 1 , is essential both for this binding and for the production of d 1 heme; replacement of His41 by Ala, Cys, Lys and Met all gave nonfunctional ... information (Doc. S1, S2 and S3). Cloning of P. pantotrophus nirF and nirF variants The nirF ORF was amplified from P. pantotrophus genomic DNA using SB5 and SB6, digeste...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx
... high-affinity binding of Man-6-P-bearing ligands, but possibly not for IGF-II. The present study, employing soluble forms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of ... ectodomain of these heterodimers, resulted in the predicted amount of ligand binding. This loss of binding function observed with asym- metric heterodimers may be due...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... interact with two types of collagen I found in vivo, and the form of the collagen determines the cells synthesis and activation of MMP-2 as well as the synthesis of MT1-MMP and TIMP-1. Previously, ... which synthesis and activation of proMMP-2, as well as syn- thesis of MT1-MMP, TIMP-1 and TIMP-2, are affected by the interaction of the cell with various bio- logica...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt
... the intermediate and mature forms of Toc75 in a given supernatant or pellet fraction showed a similar pattern of protease sensitivity (e.g., compare lanes 4 and 6, or 27 and 29 of Fig. 2A) as was shown before ... for AGA, compare lanes 19 and 20, 23 and 24, 27 and 28, and 31 and 32, respectively). Furthermore, trypsin-sen- sitivity of imported proteins was also consi...
Ngày tải lên: 19/02/2014, 07:20