Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx

... concentra- tions of ouabain applied and (c) assume a similar role by putative endogenous ouabain or OLF. Ouabain- sensitive and -insensitive a- isoforms of Na + /K + -ATPase in rats All isoforms of ... chan- ges in intracellular Na + and Ca 2+ may thus be the result of a ouabain interaction with subpopulations of Na + /K + - ATPase [27]. An overall increase...

Ngày tải lên: 23/03/2014, 17:21

... case, whicharefusedtothetwoterminiofatargetprotein .With this structural constraint, it is fairly safe to assume that a change in FRET with CYIIB monitors a conformational change in TFIIB. Similar FRET-based conformational indicators ... ratio at time t and at in nity, respectively. An error bar indicates the SD of each data point from the average value. Results Design and bioch...

Ngày tải lên: 07/03/2014, 15:20

... enzymes significantly decreased the amount of the stable c 11 aggregates. A similar observation was made after incubating the sample for 5 min at 95 °Cin SDS-containing loading buffer; this result ... c 11 with 2 and 10 lgofc 1 purified in chloroform ⁄ methanol, the slower migrating c ring aggregates did not reappear (lanes 6 and 7). Lane 9, c 1 purified by extraction with chloroform...

Ngày tải lên: 07/03/2014, 21:20

... and de-branched arabinans, and heteropolysaccharides such as arabinoxylans and arabinogalactans. Arabinan is composed of a- 1,5-linked l-arabinofuranosyl units, some of which are substituted with ... with a- 1,3- and a- 1,2- linked chains of l-arabinofuranosyl residues [4,5]. Two major enzymes hydrolyse arabinan: a- l-arabinofurano- sidases (AFNs; EC 3.2.1.55) and endo-1,5 -a- l...

Ngày tải lên: 15/03/2014, 23:20

... thank Martin Hahn and Mohamed Marahiel for the kind gift of the sulfamoyl inhibitors, Helena Jan- zen for help with statistical analysis and Tulika Dhar for proof-reading of the manuscript. Funding ... sulfamoyl adenosine inhibitors induce conformational changes in the A- PCP protein that are comparable to the effect of the native substrates To investigate conformational chan...

Ngày tải lên: 29/03/2014, 08:20

... at its N-terminus instead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intact protein at its a- amino group and represents the amino terminal amino ... Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase Ina Catrein, Richard Herrma...

Ngày tải lên: 19/02/2014, 18:20

... One of the major challenges for multilingual chat realized through machine translation technology is the normalization of non-standard, self-created short-forms in the chat message to standard ... effective approach for chat normalization. We express normalization using a probabilistic model as below )|(maxarg csPs s best  and define the probability using a linear...

Ngày tải lên: 07/03/2014, 18:20

... a~ : If the partially instantiated template is for a noun phrase, apply the processor which, given an existing ,~3minal representation, instantiates a corresponding phrasal ~Iplate, to each ... syntactically legitimate variations on a giveJ, utterance, and has built in facilities to do some synon~s substitution. It has been used in a nu, ber of application domains: no...

Ngày tải lên: 09/03/2014, 01:20

Ngày tải lên: 09/03/2014, 01:20

... single boards (Li~nard and Mariani, 1982 ; Gauvain, 1983 ; Asta and Li~nard, 1979) ; in our laboratory, such modules have been integrated into a compact unit that forms an autonomous vocal processor ... which has applications in a number of varied domains : vocal command of cars, of planes, office automation and computer-aided learning (N~el et al., 1982). Whereas most...

Ngày tải lên: 24/03/2014, 05:21