Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

... CaCl2(Table 1), emphasizing the speciﬁcity of the effect of Zn 2+ .Thermal destabilization of PSP-I ⁄ PSP-II was alsonoticed at acidic pH (Fig. 2B) in the absence of Zn 2+ cations. At pH 3.8 the apparent ... concentration of the gel ﬁltrationchromatographic behaviour of PSP-I ⁄ PSP-II. Effects of Zn 2+ (B) and acidic pH (C). A 0.75 lgÆmL)1solution of 125I-labelled PSP-I ⁄ PSP-II alone (dot lines) or in the ... Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH Marı´aAsuncio´n Campanero-Rhodes1, Margarita...

Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

... in the case of the short cyclin A2 and B1mRNAs were the half-lives signiﬁcantly prolonged inG2 ⁄ M phase. This change fully accounts for the accu-mulation of these transcripts in G2 ⁄ M phase. ... half-life changes. It is possible that the majority of the regulation of these transcripts may reside in the choice of polyadenylation site. The lack of changes in mRNA stability for mRNAs,which have ... the question of how their levels decrease at the end of M-phase. Wehave to assume a prolonged period of transcriptioninhibition to explain the decay of an mRNA that ﬂuc-tuates 10-fold in the...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... (unit:C6h)1Æg)1FW):rCO2!Suc¼ rNetPhotosynthesisÀrCO2!StarchRatesof net photosynthesis (rNetPhotosynthesis) were calcu-lated as the average rate of carbon uptake during the ﬁrsthalf of the light phase (n = ... dehydrogenase, and the conversion was measured photometrically by changes in the concentration of the reduced cosubstrate NADPH. Forisomerization of fructose 6-phosphate, phosphoglucoisom-erase ... & Stitt M (1993) Reg-ulation of the expression of rbcs and other photosyn-thetic genes by carbohydrates – a mechanism for the sink regulation of photosynthesis. Plant J 3, 817–828.29 Moore...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised forobservation by microscopy and FCS ... outside of the nuage in the cytoplasm of the migrating PGC. FCS analysis revealed that Olvas–GFP diffused with a diffusion constant of 7.0 lm2Æs)1(Fig. 3A). During the measurement of the PGC, the olvas3'UTRGFP/RFP ... LSM analysis of Olvas–GFP reveals the dynamicnature of the nuage. Schematic diagram of the preparation of PGCs of medaka specimen (A). Olvas–GFP was expressed in the medakaPGC at stage 24 and...

Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... [32]. The Lsm1 and Lsm8 polypeptides are most similar in the regions of the Sm1 and Sm2 motifs. These motifsform the Sm-fold, the hallmark of the Sm-like pro-teins, consisting of a ﬁve-stranded ... sequences from other Lsm subunits, most likely the neighbouring Lsm2p and ⁄ or Lsm4p. Alternatively,Lsm1p and Lsm8p may affect the conformation of other subunits and ⁄ or of the entire complex, ... [26,27] and in P-body assembly [28],emphasizing the importance of residues outside Lsm1p and Lsm8p for the localization and function of thesecomplexes.In budding yeast, only one form of the homologousSm...

Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot

... light on the evolutionary origin and physio-logic basis of the RDH and retinaldehyde reductasemultiplicity of vertebrates, analysis of the cephalochor-date amphioxus is invaluable. Cephalochordates ... chromosome 3 and mouse chromosome 2,which would be paralogous to chromosomes 12, 7 and 10, respectively [29]. The overall analysis, examining the topology of the phylogenetic tree and the position of ... related to the RDH1–7 ⁄ 9 forms and should be considered members of separate enzymefamilies. Indeed, the position of the amphioxus enzy-mes in the phylogenetic tree implied that these familiesare...

Báo cáo khoa học: "Analysis and Synthesis of the Distribution of Consonants over Languages: A Complex Network Approach" pptx

... these 21 consonants.On the other hand Regime 2 of Figure 3(a) as wellas Regime 1 of Figure 3(b) comprises of the rest of the consonants. The point marked as x in both the ﬁgures indicates the ... correspondingto the nodes in the set VL and the other corre-sponding to the nodes in the set VC.Degree distribution of the nodes in VL: Fig-ure 2 shows the degree distribution of the nodesin ... VLwhere the x-axis denotes the degree of eachnode expressed as a fraction of the maximum de-gree and the y-axis denotes the number of nodeshaving a given degree expressed as a fraction of the...

Báo cáo khoa học: "PARSING VS. TEXT PROCESSING IN THE ANALYSIS OF DICTIONARY DEFINITIONS" pot

... a sample definition and the triples the parser found in it. ABDOMEN 0 1 N THE PART OF THE BODY BETWEEN THE THORAX AND THE PELVIS (THE) pmod (PART) (ABDOMEN 0 1 N) lm (THE) (ABDOMEN 0 1 ... extracted the set of intransitive verb definitions, suspecting that these would be the easiest to work with. This is the smallest of the four major 219 W7 part of speech categories (the others ... definitions and one vocabulary en~r. There were 128 cases of noun followed by an -/n& form; in 116 of these the -/ng form was a participle, otherwise it was the head noun. (The other case...

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... assess the effects of alter-ing the TOS motif, we mainly examined the phosphory-lation state of Ser64, as this site is late in the hierarchy, and hence ‘integrates’ the effects of phosphorylation ... substantiallydecreased the phosphorylation at Ser64, but the phos-phorylation of the FEMRI variant was similar to that of wild-type 4E-BP1, and that of the FEMNI proteinwas intermediate between the other two ... [25]; Fig. 1A). The phosphor-ylation of the two N-terminal sites in 4E-BP1(Thr37 ⁄ 46 in the human protein; Thr36 ⁄ 45 in rat4E-BP1) requires the RAIP motif [19], and their phos-phorylation is...

Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... in the maturation of the enzymes. Most o f them a ct pleiotropicallyin the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products ... previously that carbamoylphos-phate, besides being the educt for synthesis of the CNligands, may also give rise to the formation of the carbonyl ligand [12]. The thiocarboxamide of the HypEprotein ... acylphosphatase motif; in particular, R23 ispart of an anion cradle of HypF which interacts with the phosphate of carbamoylphosphate i n a crystal o f the acylphosphatase domain complexed with the...

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