Báo cáo khoa học: Enhanced apoptosis through farnesol inhibition of phospholipase D signal transduction pdf

... Enhanced apoptosis through farnesol inhibition of phospholipase D signal transduction Marcia M. Taylor1, Kendra MacDonald1, Andrew J. Morris2and Christopher R. McMaster11 ... enzymes and other proteins containingDAG-binding C1 domains [4,36,38,39], also attenuated farnesol- induced apoptosis. As DAG and b-TPA inhib-ited farnesol- induced apoptosis this implies that farnesol prevented ... radiolabeled farnesol as a probe,was not altered by the exogenous addition of theseFig. 1. DAG consumption for the synthesis of PC and PLD medi-ated turnover for the generation of a DAG-signaling...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... of tryptic peptides obtained from puriﬁed BHLmL2. Observed = mass ⁄ charge of observed peptide; Mr(expt) = observed mass of peptide; Mr (calc) = calculated mass of matched peptide, Delta = difference ... and scores of tryptic peptides obtained from puriﬁed BHAb. Observed = mass ⁄ charge of observed peptide; Mr(expt) = observed mass of peptide; Mr (calc) = calculated mass of matched peptide, Delta ... addition, no effect of EDTAkDaAB225150100755035251522515010075503525Mr 1 2 3 4 5220 kDa120 kDa60 kDa58 kDakDa Mr 1 2 3 4Fig. 3. SDS ⁄ PAGE of fractions and of puriﬁed...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... precursorsequence of conomarphin. The signal pep-tide is shadowed and the mature peptide isunderlined. The polyA signal AATAAA in the3¢-UTR is also underlined. The cDNA of con-omarphin has been deposited ... proregion of 29 residues, the conomarphinmature peptide, and the two additional residues, Leuand Val, at the C-terminus, which are cleaved duringmaturation.Fig. 2. cDNA and the deduced precursorsequence ... the cDNA structure of conomarphinwas similar to those of other conotoxin cDNAs. ThecDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 resi-dues,...

Báo cáo khoa học: NF-jB regulates the transcription of protein tyrosine kinase Tec pdf

... had declinedsharply, and no luciferase-derived signal could bedetected at day 14. In contrast, expression of thewild-type Tec promoter construct was shown to be verystable after day 7 and ... Tectranscription. (A) The chromatin of A20 cells was cross-linked,sheared and immunoprecipitated with the indicated antibodies.Input and immunoprecipitated DNAs were puriﬁed and used astemplates in PCR ... NF-jB signalingin lymphocyte development and the immune response.Moreover, the increased use of drugs inﬂuencingNF-jB signaling requires a through understanding of genes regulated by this signaling...

Báo cáo khoa học: Cell biology, regulation and inhibition of b-secretase (BACE-1) potx

... formulation of the ‘amyloid cascade’hypothesis of AD [5]. Interruption of this metaboliccascade at one of several sites could potentially reducethe amyloid burden, and slow or even reverse thedevastating ... Ab peptides of 40 and 42 amino acids. Theamino acid sequences of Ab and around the scissile bonds are indicated by the one letter code for amino acids. The sequence shown is thewild-type sequence. ... efforts have been devoted to an understanding of its biol-ogy and structure in the subsequent decade. This has paid off in manyrespects, as it has been cloned, its structure solved, novel physiological...

Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot

... for our model of distinct signal transduction pathways leading to the independent activa-tion of the tobacco PR-1a gene by SA and by an unknownHR released signal, we have made use of transgenic ... would favor analternative explanation. In view of the results presented, itseems plausible that two distinct signal transduction path-ways could be targeted by SA and by signals released duringthe ... DNAisolated from P. putida by the alkaline lysis procedure. Asingle reaction product of 1.3 kb was detected on ethidiumbromide stained agarose gels. The PCR product wassubcloned into pUC19 cleaved...

Báo cáo khoa học: Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 ﬁbroblasts due to repression of carbonic anhydrase III docx

... available:Fig. S1. DsiRNA-mediated knockdown of caIIImRNA and protein and enhanced caspase 3 catalyticactivity in Rat1 cells.Fig. S2. DsiRNA-mediated knockdown of caIIImRNA and protein and caspase ... (Macherey-Nagal, Du¨ren,Germany).Cell cultureRat1 and EcoPak2 cells were cultured in complete mediumcomprising Dulbecco’s modiﬁed Eagle’s medium (DMEM,Evi1 enhanced oxidant-induced apoptosis ... performed in quadruplicate and errorbars the standard deviation. (B) Histogram of the percentage viability (MTT assay) of the indicated untreated (black columns) and 16 h of 750 lM H2O2treated...

Tài liệu Báo cáo khoa học: Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation pdf

... coli.Results and DiscussionThree-dimensional model of Ochr-MPHThe three-dimensional ( 3D) structure of Ochr-MPHwas modeled using the crystal structure of MPH (PDBID: 1P9E) obtained from Pseudomonas ... model for Ochr-MPH isshown in Fig. 1; it can be described as an ab ⁄ ba sand-wich typical of the metallo-hydrolase ⁄ oxidoreductasefold [6]. The ﬁnal model of Ochr-MPH, as determinedusing discovery ... wasthen assayed using the standard enzyme assay. Data points corre-spond to the mean values of three independent experiments.Fig. 6. Temperature-induced unfolding measured using CD spec-troscopy...

Tài liệu Báo cáo khoa học: Temperature compensation through systems biology pptx

... catalyzed process i.KI, inhibition constant used in negative feedforward ⁄feedback loop. For its temperature dependence, see alsoabove description of EKiaand description of scheme 11 inthe ... Y & Westerhoff HV (1986) How do inhibitorsand modiﬁers of individual enzymes affect steady-stateﬂuxes and concentrations in metabolic systems? MathModel 7, 1173–1180.26 Dixon M, Webb EC, ... breakdown of RNA, the ribosomes catalyzing the synthesis of E, andthe proteasomes ⁄ proteases catalyzing the degradation of E. The steady-state ﬂux (J5) through step 5 for pro-ducing P is described...

Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... Calibration of the decision thresholdFor the third experiment we slightly changed ourexperimental setup. Instead of dividing datasetsduring 10-fold cross-validation into training andtest subsets ... entire dataset of 2500 words, we haveexamined boundary predictions dependent on therelative word position. In Figure 3 and 4 we haveplotted the absolute counts of correct boundaries(TP) and non-boundaries ... non-boundaries (TN) which PROMODESpredicted but not PROMODES-H, and vice versa,as continuous lines. We furthermore provided thenumber of individual predictions which were ulti-mately adopted by...

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