Báo cáo khoa học: Enhanced apoptosis through farnesol inhibition of phospholipase D signal transduction pdf

... Enhanced apoptosis through farnesol inhibition of phospholipase D signal transduction Marcia M. Taylor 1 , Kendra MacDonald 1 , Andrew J. Morris 2 and Christopher R. McMaster 1 1 ... enzymes and other proteins containing DAG-binding C1 domains [4,36,38,39], also attenuated farnesol- induced apoptosis. As DAG and b-TPA inhib- ited farnesol- induced apoptosis this impli...

Ngày tải lên: 23/03/2014, 15:21

... of tryptic peptides obtained from purified BHLmL2. Observed = mass ⁄ charge of observed peptide; Mr (expt) = observed mass of peptide; Mr (calc) = calculated mass of matched peptide, Delta = difference ... and scores of tryptic peptides obtained from purified BHAb. Observed = mass ⁄ charge of observed peptide; Mr (expt) = observed mass of peptide; Mr (calc) = calculated mass of...

Ngày tải lên: 18/02/2014, 14:20

... precursor sequence of conomarphin. The signal pep- tide is shadowed and the mature peptide is underlined. The polyA signal AATAAA in the 3¢-UTR is also underlined. The cDNA of con- omarphin has been deposited ... proregion of 29 residues, the conomarphin mature peptide, and the two additional residues, Leu and Val, at the C-terminus, which are cleaved during maturation. Fig. 2. cDNA...

Ngày tải lên: 18/02/2014, 17:20

... had declined sharply, and no luciferase-derived signal could be detected at day 14. In contrast, expression of the wild-type Tec promoter construct was shown to be very stable after day 7 and ... Tec transcription. (A) The chromatin of A20 cells was cross-linked, sheared and immunoprecipitated with the indicated antibodies. Input and immunoprecipitated DNAs were purified and used as temp...

Ngày tải lên: 07/03/2014, 00:20

... formulation of the ‘amyloid cascade’ hypothesis of AD [5]. Interruption of this metabolic cascade at one of several sites could potentially reduce the amyloid burden, and slow or even reverse the devastating ... Ab peptides of 40 and 42 amino acids. The amino acid sequences of Ab and around the scissile bonds are indicated by the one letter code for amino acids. The sequence shown...

Ngày tải lên: 07/03/2014, 00:20

... for our model of distinct signal transduction pathways leading to the independent activa- tion of the tobacco PR-1a gene by SA and by an unknown HR released signal, we have made use of transgenic ... would favor an alternative explanation. In view of the results presented, it seems plausible that two distinct signal transduction path- ways could be targeted by SA and by signa...

Ngày tải lên: 23/03/2014, 15:21

... available: Fig. S1. DsiRNA-mediated knockdown of caIII mRNA and protein and enhanced caspase 3 catalytic activity in Rat1 cells. Fig. S2. DsiRNA-mediated knockdown of caIII mRNA and protein and caspase ... (Macherey-Nagal, Du ¨ ren, Germany). Cell culture Rat1 and EcoPak2 cells were cultured in complete medium comprising Dulbecco’s modified Eagle’s medium (DMEM, Evi1 enhanced oxidant-i...

Ngày tải lên: 29/03/2014, 08:20

... coli. Results and Discussion Three-dimensional model of Ochr-MPH The three-dimensional ( 3D) structure of Ochr-MPH was modeled using the crystal structure of MPH (PDB ID: 1P9E) obtained from Pseudomonas ... model for Ochr-MPH is shown in Fig. 1; it can be described as an ab ⁄ ba sand- wich typical of the metallo-hydrolase ⁄ oxidoreductase fold [6]. The final model of Ochr-MPH, as det...

Ngày tải lên: 15/02/2014, 01:20

... catalyzed process i. K I , inhibition constant used in negative feedforward ⁄ feedback loop. For its temperature dependence, see also above description of E K i a and description of scheme 11 in the ... Y & Westerhoff HV (1986) How do inhibitors and modifiers of individual enzymes affect steady-state fluxes and concentrations in metabolic systems? Math Model 7, 1173–1180. 26 Dixon M...

Ngày tải lên: 19/02/2014, 02:20

... Calibration of the decision threshold For the third experiment we slightly changed our experimental setup. Instead of dividing datasets during 10-fold cross-validation into training and test subsets ... entire dataset of 2500 words, we have examined boundary predictions dependent on the relative word position. In Figure 3 and 4 we have plotted the absolute counts of correct boundaries...

Ngày tải lên: 20/02/2014, 04:20