Báo cáo khoa học: Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus pptx

... 4897 Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus Gabriel Z ˇ olda ´ k 1 ,Ro ´ bert S ˇ ut’a ´ k 1,2 , Maria ´ n Antalı ´ k 1,3 , Mathias Sprinzl 4 and ... the role of the homodimeric structure of NADH oxidase. Keywords: NADH oxidase; conformational dynamics; flavo- proteins; fluorescence quenching;...

Ngày tải lên: 23/03/2014, 15:21

... switch of a 5 b 1 integrin in CHO cells, suggesting also a role for Ca 2+ /calmodulin-dependent molecules in regulating the affinity state of integrins on neutrophils [48]. Although PtdIns3K has ... calmodulin and PtdIns3K. In guinea pig neutrophils, it has been demonstrated that inhibition of calmodulin results in inhibition of Rac and p21 activated kinases [50]. Involveme...

Ngày tải lên: 08/03/2014, 10:20

... fragmentation using mass spectrometry, by incubating Grx2 in standard buffer for 30 min or 2 h in the presence of n-PT. After incu- bation, standard Grx2 and fragments recovered by fil- tering the incubation ... novel monothiol glutaredoxins from Saccharomyces cerevisiae provide further insight into iron–sulfur cluster binding, oligomerization, and enzymatic activity of glutare...

Ngày tải lên: 23/03/2014, 07:20

... active uptake of TMRM into the mitochon- dria interferes with the second phase of TMA-DPH quenching (Fig. 2). Initially, there is significant inhibi- tion of the quenching in the respiring cells, ... phase of fluorescence in the Pgp-over-expressing cells or fluores- cence quenching in wild-type cells (Fig. 5). Inhibition of Pgp with cyclosporine A caused a parallel increase in...

Ngày tải lên: 29/03/2014, 08:20

... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine ... enolpyr- uvyl-accepting substrate, shikimate-3-phosphate, does not interact with R124 in the ternary complex [20]. Hence, the mechanism of binding of fosfomycin by reinforcement of...

Ngày tải lên: 19/02/2014, 13:20

... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidative stress on the surrou...

Ngày tải lên: 14/02/2014, 18:20

... have indi- cated that the CagA PY -induced cell elongation pheno- type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well- known actin-binding proteins ... phosphatases involved in this scenario, however, remain unknown and do not require Shp2. Instead, CagA PY can inhibit Src activity both by direct interaction of both proteins or by bindi...

Ngày tải lên: 14/02/2014, 19:20

... survival resulting from activation of PPARb. Activation of PPARb by LD induces CERK mRNA expression and increases CerK activity in SP1 cells To investigate the role of CerK and its regulation of Cer ... cultured human keratinocytes [14]. In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that...

Ngày tải lên: 18/02/2014, 18:20

... prepared from CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractions isolated 5 min postinjection of CT-B, and this increase was maintained for up to 60 min ... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits...

Ngày tải lên: 19/02/2014, 02:20

... vector by using Lipofectamine (Invitrogen). Cells were selected in neomycin, and clones were isolated and screened for Bcl-X L expression. Creation of TAM67-overexpressing cell line The dominant-negative ... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expressi...

Ngày tải lên: 19/02/2014, 06:20