Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx
Báo cáo khoa học: Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide potx
... effects of proline and TMAO on thestructure and/ or function of lactate dehydrogenase arebased on investigations performed by Bolen and coworkers[3,44,45]. A study of the effect of proline ... FEBS 2003 Perturbation of folding and reassociation of lactate dehydrogenase by proline and trimethylamine oxide Oscar P. Chilson and Anne E. ChilsonDepartment of Biology, Washington University, ... heart lactate dehydrogenase was inhibited by both proline and trimethylamine oxide (2M). In all instances, trimethylamine oxide was the more effective inhibitor of reactivation. Analysis of the...
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Báo cáo khoa học: Perturbation of membrane microdomains in GLC4 multidrug-resistant lung cancer cells ) modification of ABCC1 (MRP1) localization and functionality docx
... evidencedeither by the rate of efflux of pirarubicin or that of glutathione. Furthercholesterol depletion below 40% yielded both a partial shift of MRP1 tothe high-density fraction and a decrease of its ... min, and 80% deple-tion being observed after 10 min of incubation; thiswas not modified by a longer time of incubation.Effect of cholesterol depletion on the rate of MRP1-mediated efflux of PIRACells ... as a function of Triton concentra-tion, where VTa and Va° are the rates of efflux in theFig. 3. Variation of the MRP1-mediated efflux of PIRA as a function of the fraction of cholesterol...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
... the regulatory effects of miR-23a on theexpression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels.To ... because of its higher fold of upregula-tion in gastric adenocarcinoma tissues. Although theextent of upregulation of miR-23a in the four pairs of gastric tissues showed great variance because of the ... thatwere predicted by two of the algorithm programs(pictar and targetscan release 5.1) and were alsodownregulated in our cDNA microarray were selectedas candidate targets of miR-23a. Among...
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Tài liệu Báo cáo khoa học: Perturbation of membranes by the amyloid b-peptide – a molecular dynamics study pptx
... the perturbation of lipids, which is a result of both hydro-phobic mismatch of the C-terminal region of Abwithin the lipid bilayer and the favorable interaction of the N-terminal segment of ... experienced by the nearbylipids to the motion and tilting of this embeddedsegment.Thinning of the bilayer in the presence of Ab40Because Ab40 is known to disrupt the integrity of thelipid ... under-standing this phenomenon. A greater knowledge of themost basic interactions between Ab and a model mem-brane can lead to a more complete understanding of the membrane-aided assembly of Ab and...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... N-terminus of cytochrome b where it is enveloped by subunit 7. The arrowslabeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome ... overall structure of thiscomplex and that of chicken and beef [6]. In yeast and higher eukaryotes, cytochrome b is encoded by mito-chondrial DNA, while the remaining subunits of the bc1complex ... analysis by SDS/PAGE and Western blotting.SDS/PAGE and Western blottingMitochondrial membranes were analyzed by standardSDS/PAGE with 15% (w/v) acrylamide and an acryl-amide/bis-acrylamide ratio of...
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Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx
... evolutionarylines of class I of this superfamily occur, i.e. the CCP line and the KatG fusion line, as is the c ase for Neuro spora crassa[47].Evolution by the birth -and- death process in class I of thesuperfamily ... hydrogen peroxide (i.e. thereaction of catalase) and to remain peroxidatically active(oxidation of substrates with hydrogen peroxide). Incontrast, C-domains lost their enzymatic activity and probably ... [12], and thus the role of the corresponding part of the gene is alsounknown. These g ene f usions togeth er with single-copygenes of APXs and CCPs are ideal for investigating theevolution of...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of the active sites of the model of IPU and Dex49AThe active site structures of t he model of IPU ... 372, and 373are the potential catalytic r esidues of I PU. Also, Trp425 of Dex49A, which is the corresponding residue of Trp402 of IPU, is reportedly located in the vicinity of the active site and ... In the model of IPU,several aromatic and charged residues, Trp277, Tyr349, and Asp371, are p resent in t he v icinity of G ly350, and c ould b efavourable fo r binding of Glc +2 of the a-(1fi4)-linkedglucose...
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Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx
... domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocaseClaire M. L. Barrett and Colin RobinsonDepartment of Biological Sciences, University of Warwick, ... transmembrane (TM) spans of TatA and TatB, with the aim of generating structural distortions. We show that sub-stitution in TatB of three residues by glycine, or a single residue by proline, has no detectable ... domains and cytoplasmicregions of TatA and TatB, and the highly conservedresidues of TatC have also been probed [24–27]. In thisreport we have analyzed the transmembrane regions of TatA and TatB,...
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Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... Chimera1C2 encoded residues 1–505 and 653–725 of TAP1 and residues 494–639 of TAP2 and chimera 2C1 encoded residues1–493 and 640–703 of TAP2 and residues 506–652 of TAP1.The exchange was performed ... EcoRI site of pHbApr1neo [22] and sequenced fully in both directions.Chimera 1V2 encoded residues 1–652 of TAP1 and residues640–703 of TAP2 and chimera 2V1 encoded residues 1–639 of TAP2 and residues ... min, and the reaction was stopped by adding cold lysis buffer containing 1% Triton X-100 and quantitated by gamma counting.Fig. 8. Working model of the peptide translocation by wild-type TAP and...
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Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx
... inMaterials and methods. Results are the mean and standard deviation of iron internalized by normal and iron-loaded hepatocytes from threeseparate experiments, and are expressed as a percentage of the ... was approved by the AnimalExperimentation Ethics Committee of the University of Western Australia and is in accordance with the AustralianCode of Practice for the care and use of animals for ... similarcharacteristics.Effect of chelators on uptake of NTBIThe effects of the chelators on NTBI uptake by hepatocytes and Q7 hepatoma cells were marked and similar. Allchelators decreased uptake to 20% or less of the...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ