Báo cáo khoa học: Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot
Báo cáo khoa học: Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot
... (2005) 3661–3672 ª 2005 FEBS Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration Hong-Tao ... incubated with 1 mM of DA analogs for 24 h, and then the reaction mixtures were analyzed by means of SEC. (B) SDS ⁄ PAGE graph of the fractions from SEC separation of the reaction products of a-Syn with ... amide region of HSQC spectra reflect formation of imino groups during reaction of the side-chain amino groups with Q or DAQ. Reaction of a-synuclein with dopamine analogs H T. Li et al.3668 FEBS...
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Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf
... requireinteraction with, nor methylation, of the PP2A C subunit[17].Little is known about the molecular basis for the interaction of the B subunits with the AC heterodimer.None of the B subunits ... sequencesimilarities between the families, they do have signi®cantsequence homology within each family. For example, within the B56 family, each isoform shares a common core r egion of 241 amino acids with 71±88% ... for 10 min each wash. Bound proteins were theneluted by incubating the beads with 20 lLof10mMreduced glutathione in buffer A on ice for 30 min The eluted polypeptides w ere analyzed by either...
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Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx
... pre-incubated for 5 min, the reaction wasinitiated by the addition of 500 lm (dl)-homocysteine andincubated at 37 °C for a further 30 min, unless otherwisestated. The reaction was terminated by the ... shows the superimposed structures of DHPS and 5-methyltetrahydrofolate. The model of the methyltetrahydrofolate-binding site of MetS with onemolecule of the substrate included was further opti-mized ... interaction potentials producedpredicted values for the percentage inhibition of each of the tested compounds. These data were then compared with the experimentally determined data (percentageinhibition...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... FEBS 369Thermal unfolding transition in the absenceand in the presence of the different ligands The influence of ligand binding on the thermal stability of C-LytA was studied by monitoring the CD ... but also at the active site [18]. There-fore, inhibition of the cell wall lytic activity of Pce by atropine and ipratropium could take place by interfer-ence with the attachment to the choline-containingteichoic ... vitro prop-erties of these compounds. It is worth noting that the extent of the effect of these analogs on the pneumo-coccal cultures is very dependent on the metabolicstate of the cell. As shown...
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Tài liệu Báo cáo khoa học: Inhibition of pea ferredoxin–NADP(H) reductase by Zn-ferrocyanide docx
... 2B). The addition of 20 lMZn-ferrocyanide in the reaction medium from the beginning of the measurement leads to the appearance of the reduced form of the enzyme, even in the presence of the ... relative to the free protein c onformations [26,50]. The protein–proteininteraction also affects the microenvironments of the twoprosthetic groups. In the case of the Fd and FNR, theirredox poten ... molecular level, the reaction proceeds to the reduction of NADP+ via hydridetransfer from the N5 atom of the flavin prosthetic group.This reaction provides the NADPH necessary for CO2assimilation...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... Fe2+–EDTA. The presence of thismetal-ion complex enhanced the inhibition observed with these enzymes consistent with the o ccurrence of radical-mediated reactions. Overall, these studies ... removal of H2O2before the peroxides were u sed in other experiments. Omission of the rose bengal, photolysis in the absence of O2, or incubation of complete samples in the absence of light, ... stoichi-ometry detected with GAPDH is consistent with the occurrence of both reaction 1 and subsequent reaction of the s ulfenic acid formed with a second thiol to give adisulfide bond (reaction 2). Previous...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... immunoreactivity (IR). The staining was semi-quantitatively evaluated by assigning a score for the inten-sity of the IR and for the proportion of cells positivelystained. The intensity of IR (intensity ... cells. Therefore, the promoter sequence of MDR-1 ()136 to 10) wasamplified, and the luciferase reporter assay wasperformed to investigate the transcriptional regulation of P-gp by PI3K/Akt. The ... exhibiting IR; 3, 50–75% of the tumor cells exhibiting IR; 4, > 75% of the tumor cellsexhibiting IR. The overall score was the multiplication of the two. The scores were then classified as negative...
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Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... hydrolysis of [32P]ATP[cP] in the presence of 0–400 ng of IRE(Fig. 8). Compared to the amount of ATP hydrolyzed with 400 ng of IRP alone, addition of IRE increased the release of inorganic ... complex formation. Further-more, ATP-cS, but not AMP-PNP, inhibited IRP–IREbinding in extracts from fibroblasts treated with the analogs in culture [41]. Therefore, the 30% inhibition of binding ... com-plex. Enhancement of ATP hydrolysis by IRE in the presence of IRP-1, but absence of hydrolysis in the presence of IRE alone, suggests that ATP may interact with the complex. However, the enhancement...
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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... only by an excess of the same, un-labeled peptide, but also by an excess of the unlabeledpeptide from the other binding sites (data not shown). The validity of the results obtained for the binding of ... channels are formed by 4 of such subunits involving the C-terminal assemblydomain. (C) Magnified sections of the peptide array: The numbers indicate the first residue of the 15-mer peptides. In the first ... medi-ated by conformational changes in the CaM ⁄ SK2 com-plex induced by binding of at least one Ca2+ion to the EF-hand motifs in the N-terminal domain of CaM[7,8]. In the deactivated state of rSK2...
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Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf
... generated in the intestinal scrapings by a-amylase present in the hetero-geneous sample or by activity in the C-terminaldomain of the full-size protein, thereby accounting for the inhibition by ‘acarbose’ ... 80% of the maltaseactivity, while MGA accounts for all glucoamylaseactivity, 20% of the maltase activity, and 1% of the sucrase activity [10]. Together, these two enzymes forma complex in the ... occurs in the lumen of the small intestinewhere the endoglycosidase a-amylase (EC 3.2.1.1)hydrolyzes the internal a-(1–4) linkages of starch whilebypassing the a-(1–6) linkages of the amylopectin...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíBT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ