Báo cáo khoa học: B1–Proteases as Molecular Targets of Drug Development B1-001 DPP-IV structure and inhibitor design ppt

... names PAS-40, PAS-52, PAS-70, PAS-110, PAS-151 and PAS-170. (PrtA Substrate molar mass). The N-ter- minal amino acid sequence of two PAS proteins, PAS-52 and PAS- 110, identified the former as Manduca ... new inhibitors of proteases from various sources. Among known protease inhibitors from fungi are, yeasts inhibitors of proteinases A (asparagine protease) and B (serine protease...

Ngày tải lên: 23/03/2014, 15:20

... addition of inhibitors. Influence of ER a1,2-mannosidase I inhibition on ER exit of amino acid-substituted ASAs In order to investigate whether stabilization of mutant ASAs through inhibition of ER ... Effects of protease or phosphatase inhibitors on the stability of mutant ASAs. Ltk – cells stably expressing the indicated amino acid-substituted ASAs were incuba- ted in the prese...

Ngày tải lên: 07/03/2014, 17:20

... percentage of the available words to ob- tain the same classification accuracy as with the full feature set, even in high-accuracy cases such as PBA and BL. The effectiveness of a small subset of features ... related tasks of movie review and political party classification. We use SVM light (Joachims, 1999) for SVM and WEKA toolkit (Witten and Frank, 2005; Hall et al., 2009)...

Ngày tải lên: 17/03/2014, 00:20

... region. 1.0 1.2 0.8 A570 0.4 0.6 0.2 0 0.5 1.0 1.5 90 80 70 60 40 50 30 20 10 0 A570 Number of colonies 0 0.5 1.0 1.5 2.0 A570 0 ASO-NC miR-23a ASO 24 h AB 48 h 72 h * * * * pcDNA3 pri-23a ASO-NC miR-23a ASO pcDNA3 pri-23a ASO-NC miR-23a ASO pcDNA3 pri-23a ASO-NC miR-23a ASO pcDNA3 ... and activated T-cell populations [21]. In gastric cancer, IL6 contains polymorphisms [22]. The expressio...

Ngày tải lên: 18/02/2014, 04:20

... range of chelators. Toxicity studies. Aspartate aminotransferase (AST) release from cells was measured using the optimized AST kit purchased from Sigma. This was carried out at every step of the ... (mobilization studies). AST release was measured at every step of the experiments as described in Materials and methods. Results are the mean and standard deviation from a typical expe...

Ngày tải lên: 20/02/2014, 11:20

... tree through a path of non-terminals and a list of base phrase chunks. Prior kernel-based methods for this task focus on using individual tree kernels to exploit tree structure- related features. ... such as rule-based (MUC, 1987-1998; Miller et al., 2000), feature-based (Kambhatla 2004; Zhou et al., 2005) and kernel-based (Zelenko et al., 2003; Culotta and Sorensen, 2004...

Ngày tải lên: 20/02/2014, 12:20

... c 550 was detected in periplasmic extracts of cells containing pHP86 (H. pylori ResBC) and pKPD1 (cytochrome c 550 ) and was quantified spectroscopically (Fig. 2). The yield was approximately 0.6% of ... level of spontaneous heme attachment. Uncata- lyzed heme attachment to cytochrome c 552 is known to occur in the E. coli cytoplasm [9], and a small amount of cytoplasmic contamin...

Ngày tải lên: 06/03/2014, 09:22

... similar to those of FEN-1 and EXO-1, and the molecular mass of DmGEN was found to be close to that of EXO-1. Therefore, we pro- posed a new class (class IV) to categorize DmGEN and SEND-1, which ... BF2 and BF3 – were 15, 17 and 19 bp, respectively (Fig. 7A). The single-stran- ded region of the BF structure was 19 bp, and the gap sizes of the blocked strand of BF1,...

Ngày tải lên: 07/03/2014, 10:20

... treated as a part of researches on dialogue models such as a finite-state model, a frame-based 229 model (Goddeau, 1996), and a plan-based model (Litman, 1987). However, a finite-state model has ... given task by using shared domain knowledge. Since a frame-based model is more flexible than a finite-state model and is more easy-implementable than a plan-based model, we adopt...

Ngày tải lên: 17/03/2014, 02:20

... release of the noco- dazole block. However, in the presence of curcumin, the percentage of cells in the mitotic phase was 83% and 81% after 4 and 8 h of block release. Thus, treat- ment of cells ... combination of 8 lm curcumin and 2 nm paclitaxel was found to be 3.1 ± 1.5. The proliferation of MCF- 7 cells was inhibited by 22% and 24% in the presence of 2 and 3 nm v...

Ngày tải lên: 23/03/2014, 03:20