Báo cáo khoa học: Actin mutations in hypertrophic and dilated cardiomyopathy cause inefficient protein folding and perturbed filament formation pdf

... with phalloidin-stained actin filaments, indicating correct processing and filament formation of tagged actin. The Tyr166Cys, Ala295Ser and Ala331Pro mutant actin proteins colocalized with actin filaments ... variant actin protein to incor- porate into filaments in the cytoskeleton. Results Some mutant actin proteins display perturbed interaction kinetics with the TRiC...

Ngày tải lên: 23/03/2014, 13:20

... structure is essential for folding both in vivo and in vitro,aswellasfor maintaining CPY in an inactive form [4–6]. The mature and precursor forms are glycoproteins [7], which contain % 16% carbohydrates ... the unfolded states of proteins [16,17], and protein folding pathways [18]. Our analytical techniques involved the use of differential scanning calorimetry (DSC) and...

Ngày tải lên: 07/03/2014, 21:20

... chromophore, and its extended, rigid conformation in the a-CPC binding site. There are 19 arginines, 13 lysines, three histidines and two tryptophans in CpcE, and 13 lysines, 10 argi- nines, one ... with CpcE, indicating that the 53 amino acids in the C-terminal region of CpcF are important for CpcF folding. PCB binding to CpcE and CpcF Work with the isomerizing lyase, PecE ⁄...

Ngày tải lên: 23/03/2014, 10:21

... contamination were followed during the preparation and storage of protein and buffer solutions [32]. Calcium-loaded protein refers to the protein in the presence of 40 mm CaCl 2 . Chemicals and ... [acrylamide] (23 data points) [43]. GdmCl-induced unfolding and refolding For equilibrium transition studies, protein samples (final concentration 40–50 lgÆmL )1 ) were incubated a...

Ngày tải lên: 30/03/2014, 04:20

... in the N termini and the proline-rich regions in the C termini of mammalian p roacrosins. In addition, we found that the 28-kDa vitelline coat protein is capable of binding to the above proline-rich ... fusion protein (Fig. 4B), indicating that the 28-kDa protein of the vitelline coat has an ability to bind to the proline-rich region present in the L1(DL2) domain of the spermo...

Ngày tải lên: 17/03/2014, 11:20

... The bound actin was eluted with G -actin buffer containing 50% formamide and dia- lysed against G -actin buffer overnight. Protein extracts, PAGE, and immunoblotting Yeast cells growing in exponential ... C-terminal actin- binding submod- ule (CABS) that contains a novel G -actin- binding domain, which we call a verprolin homology 2 C-terminal (VH2-C) domain; and a second submo...

Ngày tải lên: 18/02/2014, 16:20

... Papilio polyxenes is involved in furano- coumarin metabolism. It has been demonstrated that three amino acids are involved in protein structure stability (F116, H117 and F484) and two in substrate specificity ... from the house fly: CYP6A1 metabolizes aldrin, heptachlor [8], terpenoids [9] and diazinon [10] and CYP12A1 metabolizes aldrin, heptachlor, diazinon and azinphosmethy...

Ngày tải lên: 19/02/2014, 12:20

... obtained from different clones of the same cell line, or among data obtained in single controls and in single cell lines affected by the same mtDNA mutation, we decided to carry out the following experiments ... markedly increased in cells carrying the 3460 ⁄ ND1 and 11778 ⁄ ND4 mutations, which once again showed similar behavior. A 12-h incubation in galactose caused a significan...

Ngày tải lên: 19/02/2014, 16:20

... [16] that its folding does not proceed in a vectorial, domainwise process from the N terminus to the C terminus. Instead, folding occurs via intermediates with disulfide bridges involving distant parts ... Glucosidase II then removes the remaining terminal glucose with the consequence that newly synthesized proteins no longer bind to the lectins and leave the ER. In case a protein...

Ngày tải lên: 07/03/2014, 17:20

... Histochem- ical staining indicated that AtHELPS is mainly expressed in the young seedlings and vascular tissues of leaves and roots. Using both helps mutants and overexpression lines, we observed that, in the ... OE line seedlings on normal Murashige and Skoog (MS) plates and in a medium con- taining 100 l M K + (LK). The steady-state flux profile of K + was examined by continuo...

Ngày tải lên: 14/02/2014, 18:20