Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

... with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala ... increase in the mutant mice, the disruption of DEC1 might be compensated by DEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The supp...

Ngày tải lên: 19/02/2014, 16:20

... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and simulations of the sub- strate–enzyme interactions of the wild-type ... F200K and P2. Furthermore, the F193A and W373K muta- tions do not result in any change in the thermostability of the enzyme (Table S2), and thermal...

Ngày tải lên: 16/03/2014, 04:20

... in Caenorhabditis elegans. Nat Genet 17, 119–121. J. Ku ¨ hnl et al. Methylmalonyl-CoA epimerase from C. elegans FEBS Journal 272 (2005) 1465–1477 ª 2005 FEBS 1477 Functional analysis of the methylmalonyl-CoA ... carboxylase catalyses the formation of the S-epimer of methylmalonyl-CoA. For further catalysis by the vitamin B12-dependent Keywords Caenorhabditis...

Ngày tải lên: 23/03/2014, 13:20

... The bars represent the mean percentage survival of two samples of bacteria and the error bars the SDs of these means. Percentage survival was calculated from the fraction of treated cells surviving ... of the bacteria with the proteins, no reduction of the XTT was observed (data not shown). This suggests that the bacteria were now dead and also that the reduc...

Ngày tải lên: 23/03/2014, 07:20

... in the binding of the ligand to the other repeats that share a common epi- tope. Other inhibition mechanisms cannot be excluded; among these, there is the possibility that, through the effect of ... the effect of the proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fn may be sterically hindered in the presence of t...

Ngày tải lên: 06/03/2014, 22:21

... estimated from Hanes plots. Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... qor genes and their flanking regions. The plasmid pBG3a was digested with XhoIandDraIII to remove nptII. After the removal of the 3¢ overhang and the filling...

Ngày tải lên: 17/03/2014, 10:20

... in C. elegans to test their roles in 5-FU function and were able to identify homologs for most of them in C. elegans. To explore the functional relationships of these pyrimidine biosynthesis ... that knockdown of these two genes is very specific. To more precisely understand the functions of these uridine kinase homologs, we analyzed their enzyme activity in vitro. The prot...

Ngày tải lên: 30/03/2014, 01:20

... for the chemotherapeutic treatment of cancer and viral diseases because they catalyse the addition of the first phosphate group to the nucleoside analogue. This primes the nucleoside for further ... in hydrogen bonding. The result that E52Q did not show any activity must therefore be a consequence of the reactivity of the functional group and further support the hyp...

Ngày tải lên: 19/02/2014, 02:20

... amplitudes and rate constants of the two other phases. The above should be gov- erned by the balance between the rate of reduction and oxidation of the acceptor side of PSII by the avail- able electron ... half-time of the J–I phase were both decreased with DCMU, demonstrating that the J–I rise is not directly related to the reduction of the PQ pool. A further...

Ngày tải lên: 19/02/2014, 05:20

... centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal structure of the Ydc2–junction complex. In this study the function of several ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pai...

Ngày tải lên: 20/02/2014, 11:20