Báo cáo khoa học: Flavonol 3-O-glycoside hydroxycinnamoyltransferases from Scots pine (Pinus sylvestris L ) docx

... 39, 15–20. Hydroxycinnamoyltransferases from Scots pine F. Kaffarnik et al. 1424 FEBS Journal 272 (200 5) 1415–1424 ª 2005 FEBS Flavonol 3-O-glycoside hydroxycinnamoyltransferases from Scots pine (Pinus sylvestris L. ) Florian ... NaCl, 10% (v ⁄ v) glycerol, 1 mm DTE and Com- plete Protease Inhibitor cocktail (one tablet per 50 mL). A volume of 250 lL of a de...

Ngày tải lên: 23/03/2014, 13:20

... cloramphenicol (34 lgÆmL )1 ), arabinose (1 mgÆmL )1 ) and tetracycline (1 ngÆmL )1 )at30°C. Growth was followed up to D 600 nm = 0.6, at that point 100 lm isopropyl thio- b-d-galactoside ... I (10 lgÆmL )1 extract), MgCl 2 (5 mm), and a mixture of protease inhibitors, antipain and leupeptin (2 lgÆmL )1 extract). Cells were disrupted in a French press cell (at 19 000...

Ngày tải lên: 18/02/2014, 04:20

... 2), S37–44. 24 Kell DB. (200 6) Metabolomics, modelling and machine learning in systems biology: towards an understanding of the languages of cells. The 2005 Theodor Bu ¨ cher lecture. FEBS J 273, ... analytical techniques. Abbreviations SBML, Systems Biology Markup Language; SBRML, Systems Biology Results Markup Language; STRENDA, Standards for Reporting Enzymology Data; XML, Extensible...

Ngày tải lên: 18/02/2014, 04:20

... synchronization of molecular events. In eukaryotes, a complex molecular machinery involving multiply phosphorylated proteins allows DNA replication to start simultaneously from multiple origins of replication ... (nuclear factor of activated T cells) by calcineurin (CaN) at several Ser residues induces a conformational change that exposes a nuclear localization signal (NLS), leading to nucl...

Ngày tải lên: 18/02/2014, 08:20

... ProteoExtract Subcellu- lar Proteome Extraction Kit (Calbiochem, La Jolla, CA, USA) was used to extract proteins from mammalian cells according to their cytosolic or cytoskeletal subcellular localization. ... C) Subcellular protein fractionation. Chemical fraction of cell pellets after FLAG–s-DAPK transfection into a cytosolic (A) and cytoskeletal (B) fractions for Flag–s-DAPK or Flag–s-DAP...

Ngày tải lên: 18/02/2014, 17:20

... expression levels of metallothio- nein (MT) in HepG2 cells. (A) Ethanol (5 m M), 4-methylpyrazole hydrochloride (4-MP) ⁄ ethanol (5 l M ⁄ 5mM), disulfiram ⁄ ethanol (5 l M ⁄ 5mM) or acetaldehyde (1 mM) ... of available intracellular zinc in HepG2 cells. (A) HepG2 cells (1 · 10 6 ) were treated with acetal- dehyde (1 m M) or acrolein (10 lM) for 30 min. The cells were col- lected and la...

Ngày tải lên: 19/02/2014, 06:20

... ice/ethanol bath and stored at )7 0 °C in aliquots. Epimerase purified from wild- type cells will be referred to as wild-type protein while that purified from transformed PJB5 cells will be described ... was also assayed for glucose or galactose induced cell lysate. The rate of conversion of a- D -glucose to b- D -glucose in induced and uninduced cell lysates are 3.5 lmolÆmin )1 and...

Ngày tải lên: 19/02/2014, 12:20

... activity of the full length protein was approximately twofold lower than that of the C-terminal domain alone. However, as the calculated molecular mass (52 438 Da) of the His-tagged full length protein ... Journal 272 (200 5) 2828–2837 ª 2005 FEBS MIC2067(DE 3) was transformed with each constructed plasmid, spread on Luria agar plates containing 50 lgÆ mL )1 ampicillin and 30 lgÆmL...

Ngày tải lên: 19/02/2014, 18:20

... analysis Sphingolipid analysis of yeast cells was carried out essen- tially as described previously [21]. LCBs were liberated from yeast cells by alkaline hydrolysis and extracted with chloroform ... D4-desaturase from the microalga Pavlova lutheri. FEBS Lett 553, 440–444. 15 Pereira SL, Leonard AE, Huang Y-S, Chuang L- T & Mukerji P (200 4) Identification of two novel microalgal e...

Ngày tải lên: 20/02/2014, 01:20

... Sample User 1 Word 1 自然语言(Natural Language) Word 2 人工智能(Artificial Intelligence) Word 3 机器翻译(Machine Translation) Word 2 人工智能(Artificial Intelligence) Word 4 信息检索(Information Retrieval) ... (Ericsson) Top 5 Candidates After Re-ranking 北电 (Nortel) 索尼爱立信 (Sony Ericsson) 中兴 (ZTE Corporation) 索爱 (Sony Ericsson) 基站 (Base Station) 阿尔卡特 (Alcate...

Ngày tải lên: 20/02/2014, 04:20