Báo cáo khoa học: Flavonol 3-O-glycoside hydroxycinnamoyltransferases from Scots pine (Pinus sylvestris L ) docx
Báo cáo khoa học: Flavonol 3-O-glycoside hydroxycinnamoyltransferases from Scots pine (Pinus sylvestris L.) docx
... 39,
15–20.
Hydroxycinnamoyltransferases from Scots pine F. Kaffarnik et al.
1424 FEBS Journal 272 (200 5) 1415–1424 ª 2005 FEBS
Flavonol 3-O-glycoside hydroxycinnamoyltransferases
from Scots pine (Pinus sylvestris L. )
Florian ... NaCl, 10% (v ⁄ v) glycerol, 1 mm DTE and Com-
plete Protease Inhibitor cocktail (one tablet per 50 mL). A
volume of 250 lL of a de...
Tài liệu Báo cáo khoa học: The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity docx
... cloramphenicol (34 lgÆmL
)1
), arabinose (1 mgÆmL
)1
) and
tetracycline (1 ngÆmL
)1
)at30°C. Growth was followed
up to D
600 nm
= 0.6, at that point 100 lm isopropyl thio-
b-d-galactoside ... I (10 lgÆmL
)1
extract),
MgCl
2
(5 mm), and a mixture of protease inhibitors,
antipain and leupeptin (2 lgÆmL
)1
extract). Cells were
disrupted in a French press cell (at 19 000...
Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf
... 2), S37–44.
24 Kell DB. (200 6) Metabolomics, modelling and machine
learning in systems biology: towards an understanding
of the languages of cells. The 2005 Theodor Bu
¨
cher
lecture. FEBS J 273, ... analytical techniques.
Abbreviations
SBML, Systems Biology Markup Language; SBRML, Systems Biology Results Markup Language; STRENDA, Standards for Reporting
Enzymology Data; XML, Extensible...
Tài liệu Báo cáo khoa học: Multisite protein phosphorylation – from molecular mechanisms to kinetic models pdf
... synchronization of molecular
events. In eukaryotes, a complex molecular machinery
involving multiply phosphorylated proteins allows
DNA replication to start simultaneously from multiple
origins of replication ... (nuclear factor of activated T cells) by calcineurin (CaN) at
several Ser residues induces a conformational change that exposes
a nuclear localization signal (NLS), leading to nucl...
Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf
... ProteoExtract Subcellu-
lar Proteome Extraction Kit (Calbiochem, La Jolla, CA,
USA) was used to extract proteins from mammalian cells
according to their cytosolic or cytoskeletal subcellular
localization. ... C)
Subcellular protein fractionation. Chemical fraction of cell pellets after FLAG–s-DAPK transfection into a cytosolic (A) and cytoskeletal (B)
fractions for Flag–s-DAPK or Flag–s-DAP...
Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx
... expression levels of metallothio-
nein (MT) in HepG2 cells. (A) Ethanol (5 m
M), 4-methylpyrazole
hydrochloride (4-MP) ⁄ ethanol (5 l
M ⁄ 5mM), disulfiram ⁄ ethanol
(5 l
M ⁄ 5mM) or acetaldehyde (1 mM) ... of available intracellular zinc
in HepG2 cells. (A) HepG2 cells (1 · 10
6
) were treated with acetal-
dehyde (1 m
M) or acrolein (10 lM) for 30 min. The cells were col-
lected and la...
Tài liệu Báo cáo khóa học: UDPgalactose 4-epimerase from Saccharomyces cerevisiae A bifunctional enzyme with aldose 1-epimerase activity docx
... ice/ethanol bath and
stored at )7 0 °C in aliquots. Epimerase purified from wild-
type cells will be referred to as wild-type protein while that
purified from transformed PJB5 cells will be described ... was also assayed for glucose
or galactose induced cell lysate. The rate of conversion of
a-
D
-glucose to b-
D
-glucose in induced and uninduced cell
lysates are 3.5 lmolÆmin
)1
and...
Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... activity of the full length protein
was approximately twofold lower than that of the
C-terminal domain alone. However, as the calculated
molecular mass (52 438 Da) of the His-tagged full
length protein ... Journal 272 (200 5) 2828–2837 ª 2005 FEBS
MIC2067(DE 3) was transformed with each constructed
plasmid, spread on Luria agar plates containing 50 lgÆ mL
)1
ampicillin and 30 lgÆmL...
Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx
... analysis
Sphingolipid analysis of yeast cells was carried out essen-
tially as described previously [21]. LCBs were liberated
from yeast cells by alkaline hydrolysis and extracted with
chloroform ... D4-desaturase from the microalga Pavlova lutheri.
FEBS Lett 553, 440–444.
15 Pereira SL, Leonard AE, Huang Y-S, Chuang L- T &
Mukerji P (200 4) Identification of two novel microalgal
e...
Tài liệu Báo cáo khoa học: "Growing Related Words from Seed via User Behaviors: A Re-ranking Based Approach" pdf
... Sample
User
1
Word
1
自然语言(Natural Language)
Word
2
人工智能(Artificial Intelligence)
Word
3
机器翻译(Machine Translation)
Word
2
人工智能(Artificial Intelligence)
Word
4
信息检索(Information Retrieval) ... (Ericsson)
Top 5 Candidates
After Re-ranking
北电 (Nortel)
索尼爱立信 (Sony
Ericsson)
中兴 (ZTE Corporation)
索爱 (Sony Ericsson)
基站 (Base Station)
阿尔卡特 (Alcate...