Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot

... Vincenzo et al. Adenylation domains of freestanding NRPS enzymes In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like ... among the freestanding NRPS A domains and the correspond- ing domains of the traditional NRPS in a phylo- genetic tree, the...

Ngày tải lên: 23/03/2014, 13:20

... sufficient to explain the complete inability of a saturating amount of DNA substrate to inhibit RNA splicing (Fig. 6B) and therefore it seems reasonable to make the working hypothesis that the RNA and ... 10 9 M )1 Æmin )1 under similar conditions [38] to these. We measured the rate constant for the binding of DNA to protein kinetically and obtained a value of at le...

Ngày tải lên: 17/03/2014, 10:20

... [9]. In C. reinhardtii ,the5 ¢ UTR of the psbA mRNA encoding the D1 protein of the photosystem (PS) II reaction centre was shown to interact with RB47, a member of the polyA-binding protein family, ... contained the pro- motor of the T7 RNA polymerase fused to the 5¢ end of the described fragments. In vitro transcription of RNA, UV cross-linking of RNAs...

Ngày tải lên: 17/03/2014, 11:20

... aiming to minimize the effect of the different experimental systems on the findings obtained. In addition, the recent development of an in vivo model should allow the significance of the in vitro findings ... network via the motor protein dynein dur- ing infectious entry [75]. The C-terminal 40 amino acids of L2 were found to be essential for interaction with t...

Ngày tải lên: 23/03/2014, 04:20

... can relay informa- tion to other distant regions of the protein. Also the large increase in the mobility of some regions can be expected to occur due to a redistribution of the pro- tein’s configurational ... could indirectly affect the deproto- nation and protonation rates of Ser195 thus reducing the kinetics of catalysis. The results also suggest that the dy...

Ngày tải lên: 19/02/2014, 05:20

... Rf, with changing N, express the changes of the status of the morphemes of each type in the terminology. In terminology, Pf can be interpreted as the probability of in- corporating new conceptual ... nature of the sample. Located in the intersection of these two backgrounds, the aim of the present study is twofold, i.e. (1) to introduce a quantit...

Ngày tải lên: 20/02/2014, 18:20

... uptake into the regenerative limb of the cycle resulting in the synthesis of ribulose 1,5-bisphosphate, continuing the cycle. In eukaryotic organisms the Calvin cycle is located within the chloroplast ... as being some combination of the following: (a) production of redox potential in the form of NADPH; (b) production of glycolytic intermediates, reducing th...

Ngày tải lên: 20/02/2014, 23:20

... (Fig. 6). In mammals, insulin-like growth factor-binding proteins, which con- tain the IGFBP motif, modulate the actions of the insulin-like growth factors in endocrine, paracrine, and autocrine systems ... demonstrated the pres- ence of minor amounts of cysteic acid, suggesting the presence of disulfide bond-containing proteins in insect cuticles [43,44]. Nucleotide se...

Ngày tải lên: 07/03/2014, 21:20

... atoms from acetaldehyde are incorporated into the alcohol formation in the other metabolite. The assumption was proved by adding acetaldehyde (0.5 m M and 5 m M ) to the biotransformation. The ... at k max ¼ 265 nm during the first 24 h of the biotransformation. Furthermore the consumption of 2-butynedioate could not be monitored by 1 H-NMR due to the lack of prot...

Ngày tải lên: 08/03/2014, 08:20

... correspondingly complex. The sequences are highly descriptive, naming parts in terms of their function and relation to other parts, and also describing the status of parts and other objects in the ... Nominalizations. The increased frequency of preposi- tional modifiers in the equipment failure messages was traced to the frequent use of nominalizations in...

Ngày tải lên: 08/03/2014, 18:20