Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx

... 2004 Synergy in activation of the IFN-b gene (Eur. J. Biochem. 271) 3703 Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon- b ... arrange- ment of the cis-acting elements in the promoter and to the nature of the factors they bind. To investigate the effe...

Ngày tải lên: 23/03/2014, 13:20

... (from 2 to 9 in increments of 0.5) in buffers containing 150 mm NaCl by incubating at 37 °C for 3 h. Again, there was no evidence for the production of a specific product; instead, the reaction mixture ... varying amounts of BTH and for different times as indicated: (B) 0.05 lg of BTH, 5 min incuba- tion; (C) 0.5 lg of BTH, 5 min incubation; (D) 5 lg of BTH, 5 min i...

Ngày tải lên: 29/03/2014, 08:20

... switch in renal cell carcinoma (RCC) is attributed to the inactivation of the von Hippel–Lindau tumor suppressor, stabilization of hypoxia inducible factor- 1 transcription factor and increased ... potent and versatile angiogenesis inhibitor that is critical for the maintenance of the antiangiogen- ic microenvironment in multiple organ sites, including breast, brain...

Ngày tải lên: 07/03/2014, 05:20

... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the ... the b-strands b2, b3, b4 and b8 of the (b ⁄ a) 8 barrel domain, and for rBAT also with the short stretch near the C-terminus of domain B [9,19]. From the s...

Ngày tải lên: 18/02/2014, 13:20

... changes in the cytoskeleton that may occur during the initial 6 h window of infection. We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 min and 6 h postinfection, ... fully understand HSV-1 infection of all target cells, includ- ing RPE cells, our study provides new starting points for understanding viral pathogenesis in the r...

Ngày tải lên: 18/02/2014, 14:20

... set of control data was obtained in the absence of the protein. The data set obtained in the absence of the protein was then subtracted from the corresponding data set obtained in the presence of ... (association and dissociation). Therefore, the rate constants for the binding of DHNP and DHMP were fixed at the values measured for the corresponding...

Ngày tải lên: 19/02/2014, 00:20

... demonstrating that the individual binding constants of divalent metal ions for the a domain are larger than those for the b domain. Further interpretation of these data resulted in the proposal of positively ... elucidate the meta- llation mechanisms of the individual domains, in the hope, initially, of simplifying the interpretation of the metallation de...

Ngày tải lên: 19/02/2014, 00:20

... 40– 66 of the cofactor is important in the folding of the protein and that the C-terminal part of the cofactor, which is absent in the truncated form, directly interacts with the substrate-binding ... interaction between the NS2B cofactor and NS3pro seems to be important not only for the correct fold of the protease but also for the correct interact...

Ngày tải lên: 19/02/2014, 00:20

... activity in crude yeast extract as the untagged protein, indicating that the tag was not interfering with the protein activity. The tagged protein was purified and then used for further analysis. In the ... nkatÆmg )1 . The GLD1 pro- tein was tagged with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open...

Ngày tải lên: 19/02/2014, 06:20

... could participate in proton translocation or, in the case of E103, in the binding of the twin-arginine motif in the signal peptide. The absence of any translocation defects leads us to conclude that these ... 2275 Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase Claire M....

Ngày tải lên: 19/02/2014, 17:20