Báo cáo khoa học: Chaperone activity of recombinant maize chloroplast protein synthesis elongation factor, EF-Tu docx

... Chaperone activity of recombinant maize chloroplast protein synthesis elongation factor, EF-Tu Damodara Rao 1 , Ivana Momcilovic 1 , Satoru ... hypothesized that, in maize, this protein may show chaperone activity similar to prokaryotic EF-Tu [5,9–11]. Fig. 6. Effect of recombinant m aize pre -EF-Tu ( EF-Tu) on the activity of citra te synthase ... prot...

Ngày tải lên: 23/03/2014, 13:20

... scattering can be used as a measure of effectiveness of the chaperone. Using this assay we tested the relative activity of the two wheat proteins in suppressing aggregation of MDH. As shown in Fig. 5, ... its chaperone activity, and likewise, data on activity of the class II pro- teins is very limited. We prepared purified, recombinant TaHsp16.9C-I and TaHsp17.8C-II and find...

Ngày tải lên: 07/03/2014, 15:20

... Rockford, IL, USA). Bands of GST-fusion proteins were detected by staining the PVDF membranes with CBB. In vitro binding assay using recombinant proteins Ten micrograms of GST (negative control) ... epidermal growth factor, suggesting involvement of calpain 7 in the ESCRT system [28]. On the basis of the resolved 3D structure, the MIT domain of VPS4 forms three-helix bundles. ES...

Ngày tải lên: 18/02/2014, 04:20

... the existence of several types of hemin conformation in the protein pocket is consistent with this non a-specific production of biliverdin. We expected that DmDHO would produce the c-isomer of biliverdin ... spectrum of hexa- coordinated heme protein. The absorption spectrum of the ferric complex was not influenced by changing the pH of the solution. Interestingly, an EPR study...

Ngày tải lên: 19/02/2014, 12:20

... for the function of BchI among the various functions of AAA + proteins. The AAA + proteins represent one type of molecular chaperones and their function is to control the fate of proteins or DNA. ... This is done by facilitating protein folding and unfolding, assembly and disassembly of protein complexes, degradation of protein, replication and tran- scription of DNA, etc....

Ngày tải lên: 19/02/2014, 12:20

... the mechanism of action of chymotrypsin: the role of the low barrier hydrogen bond. Biochemistry 36, 4576–4584. 46. Warshel, A. (1998) Electrostatic origin of the catalytic power of enzymes and the role of ... general features of the recently determined X-ray structure of human BuChE [7,8]. In particular, most of the essential features of the catalytic site (i.e. a catalyt...

Ngày tải lên: 20/02/2014, 23:20

... refer to amino-acid residues of the C. albicans protein. (B) Alignment of the catalytic centre of the recombinantly expressed C. albicans DHODH and amino-acid sequences of different DHODH family ... of recombinant DHODH should expedite discov- ery of more potent agents for growth control strategies in C. albicans, and permit the screening of a large number of compounds, the...

Ngày tải lên: 07/03/2014, 12:20

... Devices). Results Identification of histone proteins in the hemocytes In the course of an initial proteomic inve stigation of shrimp immune function, simple one-dimensional SDS/PAGE analysis of the hemocytes soluble proteins ... the results of a proteomic investigation of hemocyte antimicrobial peptides of the P acific white shrimp, L. vannamei, and the r ole of histones as pote...

Ngày tải lên: 07/03/2014, 16:20

... supernatant. Fig. 6. Autophosphorylation of recombinant Ndk and mutant proteins. (A) Ndk and mutant proteins (1 lg) were incubated in the presence of 10 lCi of [c- 32 P]ATP in 20 lL of reaction volume. The reaction ... of 2 lL of 10% SDS. The samples were boiled for 10 min and separated by 15% SDS/PAGE. Analysis was by autoradiography. Enzymatic activity of Ndk Enzymatic ac...

Ngày tải lên: 08/03/2014, 08:20

... region, one copy of a double stranded oligonucleotide spanning the region from )70 to )36 was fused upstream of pTATALUC. Evaluation of reporter activity following transfection of A6 cells revealed ... detected in bone, cartilage and in soft tissues such as he art, kidney, and lung in a variety of species [4,6,7]. MGP is also secreted in vitro by a number of cell lines of diff...

Ngày tải lên: 08/03/2014, 10:20