Báo cáo khoa học: Cd2+-induced aggregation of Escherichia coli pyrophosphatase doc
Báo cáo khoa học: Cd2+-induced aggregation of Escherichia coli pyrophosphatase doc
... Escherichia coli inorganic pyrophosphatase
(PPase) aggregation in the presence of 2 m
M
cadmium acetate.
Fig. 2. Dependence of Escherichia coli inorganic pyrophosphatase
(PPase) aggregation on cadmium ... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia;
2
Department of Biochemistry, University of Turku, Finland
We report here...
Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot
... reaction of KMnO
4
. Nucleic Acids Research 26,
3940–3943.
49 Zamenhof PJ & Villarej. M (1972) Construction and
properties of Escherichia coli strains exhibiting alpha-
complementation of beta-galactosidase ... E109A mutant
of Escherichia coli DNA photolyase, which is catalytically active but unable
to bind the second cofactor methenyltetrahydrofolate, are described. A
sta...
Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
... The radioactivity
of gel slices was determined by Cerenkov counting. The
yield of the complex was calculated as the ratio of shifted
band radioactivity to the total radioactivity of the loaded
sample. ... Equal
mobilities of the radioactive and the protein-containing
bands indicated covalent attachment of DNA to the
enzyme. The yield of the photochemical cross-linking
reaction w...
Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase doc
... thank DST, Government of India, and
MHRD, Government of India, for financial support.
We also thank Professor Winfried Boos, University of
Konstanz, Germany, for the gift of plasmids encoding
His-tagged ... W (1991) The
malZ gene of Escherichia coli, a member of the maltose
regulon, encodes a maltodextrin glucosidase. J Biol
Chem 266, 19450–19458.
Chaperone-assisted folding of...
Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt
... specificity of hydrolytic activity, thus
avoiding the synthesis of many different oligosaccha-
ride substrates.
Previously, we reported that the functionally
unknown protein YicI of Escherichia coli ... structure of a-d-Xylp-(1 fi 6)-d-
Fruf, the transxylosylation product from fructofura-
nose, the 4-OH of the fructofuranose would interact
with Arg446 and Asp185 at subsite +1. In...
Tài liệu Báo cáo khoa học: Cosubstrate-induced dynamics of D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida ppt
... conformation of NAD
+
and the structure of the
cosubstrate-binding pocket of PpHBDH are very simi-
lar to those of PfHBDH, with a distance of about
14 A
˚
between the C2 of nicotinamide and the C6 of
the ... Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy,
University of Leipzig, Germany
2 Institute of Biochemistry, Faculty of Biosciences, Phar...
Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx
... magnification of original spectra. (D) Absorption spectra of the acid-
denatured K13N mutant (spectrum a), of the K13N mutant after 3 ms of mixing with 40 m
M sulfate (spectrum b), and after 5 s of mixing
with ... structure of cyt c A-state
FEBS Journal 273 (2006) 5347–5357 ª 2006 The Authors Journal compilation ª 2006 FEBS 5357
Salt-induced formation of the A-state of
ferricyt...
Báo cáo khoa học: Radical-induced oxidation of metformin pptx
... no
detectable effect of H
2
O
2
as an initiator of metformin
oxidation in the absence of irradiation. However, under
irradiation, there was a noticeable effect of the concentra-
tion of H
2
O
2
(0.05, ... inhibition of metformin oxida-
tion, in the presence of added hydrogen peroxide, would
come from the reaction of
Æ
OH radicals with H
2
O
2
.An
estimated value of the secon...
Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx
... Unit, Laboratory of Physiology, School of Medicine, University of Ioannina, Greece
2 Laboratory of General Biology, School of Medicine, University of Ioannina, Greece
3 Laboratory of Cellular and ... expression.
Fig. S2. Effects of VOSO
4
on IL-6 expression in
HaCaT cells.
Doc. S1. Construction of growth curves and isolation
of total RNA and RT-PCR for the expression of...
Báo cáo khoa học: Calcium-induced contraction of sarcomeres changes the regulation of mitochondrial respiration in permeabilized cardiac cells doc
... aspects of the preparation of such kind of
buffers were published recently in [69] by the author
of the software we used for our calculations (see
below).
The influence of temperature (van’t Hoff ... and Fisher test), and
a P-value of < 0.05 was taken as the level of significance.
Calculation of the free concentration of Ca
2+
Calculations of the composition of EGTA-Ca...
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