Báo cáo khoa học: Cd2+-induced aggregation of Escherichia coli pyrophosphatase doc

... Escherichia coli inorganic pyrophosphatase (PPase) aggregation in the presence of 2 m M cadmium acetate. Fig. 2. Dependence of Escherichia coli inorganic pyrophosphatase (PPase) aggregation on cadmium ... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia; 2 Department of Biochemistry, University of Turku, Finland We report here...

Ngày tải lên: 23/03/2014, 13:20

... reaction of KMnO 4 . Nucleic Acids Research 26, 3940–3943. 49 Zamenhof PJ & Villarej. M (1972) Construction and properties of Escherichia coli strains exhibiting alpha- complementation of beta-galactosidase ... E109A mutant of Escherichia coli DNA photolyase, which is catalytically active but unable to bind the second cofactor methenyltetrahydrofolate, are described. A sta...

Ngày tải lên: 16/03/2014, 18:20

... The radioactivity of gel slices was determined by Cerenkov counting. The yield of the complex was calculated as the ratio of shifted band radioactivity to the total radioactivity of the loaded sample. ... Equal mobilities of the radioactive and the protein-containing bands indicated covalent attachment of DNA to the enzyme. The yield of the photochemical cross-linking reaction w...

Ngày tải lên: 20/02/2014, 11:20

... thank DST, Government of India, and MHRD, Government of India, for financial support. We also thank Professor Winfried Boos, University of Konstanz, Germany, for the gift of plasmids encoding His-tagged ... W (1991) The malZ gene of Escherichia coli, a member of the maltose regulon, encodes a maltodextrin glucosidase. J Biol Chem 266, 19450–19458. Chaperone-assisted folding of...

Ngày tải lên: 16/03/2014, 06:20

... specificity of hydrolytic activity, thus avoiding the synthesis of many different oligosaccha- ride substrates. Previously, we reported that the functionally unknown protein YicI of Escherichia coli ... structure of a-d-Xylp-(1 fi 6)-d- Fruf, the transxylosylation product from fructofura- nose, the 4-OH of the fructofuranose would interact with Arg446 and Asp185 at subsite +1. In...

Ngày tải lên: 23/03/2014, 07:20

... conformation of NAD + and the structure of the cosubstrate-binding pocket of PpHBDH are very simi- lar to those of PfHBDH, with a distance of about 14 A ˚ between the C2 of nicotinamide and the C6 of the ... Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Germany 2 Institute of Biochemistry, Faculty of Biosciences, Phar...

Ngày tải lên: 18/02/2014, 16:20

... magnification of original spectra. (D) Absorption spectra of the acid- denatured K13N mutant (spectrum a), of the K13N mutant after 3 ms of mixing with 40 m M sulfate (spectrum b), and after 5 s of mixing with ... structure of cyt c A-state FEBS Journal 273 (2006) 5347–5357 ª 2006 The Authors Journal compilation ª 2006 FEBS 5357 Salt-induced formation of the A-state of ferricyt...

Ngày tải lên: 19/02/2014, 05:20

... no detectable effect of H 2 O 2 as an initiator of metformin oxidation in the absence of irradiation. However, under irradiation, there was a noticeable effect of the concentra- tion of H 2 O 2 (0.05, ... inhibition of metformin oxida- tion, in the presence of added hydrogen peroxide, would come from the reaction of Æ OH radicals with H 2 O 2 .An estimated value of the secon...

Ngày tải lên: 07/03/2014, 16:20

... Unit, Laboratory of Physiology, School of Medicine, University of Ioannina, Greece 2 Laboratory of General Biology, School of Medicine, University of Ioannina, Greece 3 Laboratory of Cellular and ... expression. Fig. S2. Effects of VOSO 4 on IL-6 expression in HaCaT cells. Doc. S1. Construction of growth curves and isolation of total RNA and RT-PCR for the expression of...

Ngày tải lên: 16/03/2014, 02:20

... aspects of the preparation of such kind of buffers were published recently in [69] by the author of the software we used for our calculations (see below). The influence of temperature (van’t Hoff ... and Fisher test), and a P-value of < 0.05 was taken as the level of significance. Calculation of the free concentration of Ca 2+ Calculations of the composition of EGTA-Ca...

Ngày tải lên: 16/03/2014, 22:20