... Miyamoto K, Tanaka K, Kawai M, TainakaK, Imada C, Okami Y & Inamori Y (1993) Cloning and sequence of an alkaline serine protease-encodinggene from the marine bacterium Alteromonas sp. strainO-7. ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GAAAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) and downstream ... microtiter plate protocol as described bythe manufacturer using BSA as standard.Standard enzyme assaySPRK activity was routinely measured using Suc-Ala-Ala-Pro-Phe-pNA (Sigma Aldrich) as substrate....