Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel specificity towards C-terminal glutamate residues pptx
Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel specificity towards C-terminal glutamate residues pptx
... 2011 Characterization of a digestive carboxypeptidase from the insect pest corn earworm ( Helicoverpa armigera ) with novel specificity towards C-terminal glutamate residues David P. Bown and John ... was assayed, there was a qualitative correlation between the appearance of the putative 36 kDa activated carboxypeptidase polypeptide, and the leve...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt
... as a template. The quality of the model was first assessed using procheck [32,33]. The vast majority of residues (89.7%) fall into the most favoured regions of the Ramachandran plot, 9.4% in the ... The amino acid sequence of hemerythrin from Siphonosoma cumanense. Protein Seq Data Anal 3, 141–147. 43 Yano H, Satake K, Ueno Y & Tsugita A (1991) The amino aci...
Ngày tải lên: 07/03/2014, 17:20
Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt
... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... obtain the full length sequence: OP5, 5¢-GACTGTAACGGTCATGGYACMAYGT-3¢; OP6, 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG- 3¢; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACG TG-3¢; NP4, 5¢-GACACCGTAGGTTGAGC...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
... W81, as observed with M. brassicae CSPMbraA6 [32]. We observed also that BrC15-Ac was able to displace ASA, suggesting that brominated fatty acid and ASA both associated with W81 in the same ligand ... native signal peptide was amplified by PCR using the following primers: 5¢ primer, 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae potx
... alternative pathways are avail- able, it is unlikely to play any significant role in the catabolism of the branched-chain amino acids or of methionine. Identification and mutation of residues affecting substrate ... Journal compilation ª 2011 FEBS 1853 Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae Malea M. Knee...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
... CLAP_2:AAGTCTGTCGTCAAAATGTTATGAACGTCTCTTGTCATAAAGAAAGAGAACCTCTCTTTTTAGTTTGGTTTAGATATTAAGGACAGATCCAAAATATTTG 1132 * CLAP_1:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTA AAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT 1300 CLAP_2:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTA AAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT ... CLAP_2:ATTAGTGCTATAGTTTGGGAAATATTTAGTCCTTGTTTTG...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt
... it was named PA1b for pea albumin 1b. PA1b is the result of the post-translational cleavage of the albumin proprotein PA1, also releasing a second peptide (PA 1a) . PA1b consists of 37 amino acids, ... tubes. The radioactivity was counted on a gamma counter (Riastar, Packard Instrument, USA), and each point was the mean of triplicates. Binding data were analyzed using t...
Ngày tải lên: 08/03/2014, 02:21
Báo cáo khoa học: Characterization of a membrane-bound aminopeptidase purified from Acyrthosiphon pisum midgut cells A major binding site for toxic mannose lectins pptx
... APN2 AAD31184 Ldi APN2 BAA32140 Bmo P91885 Mse APN2 CAA10950 Pxy BAA33715 Bmo AAX39866 Tni APN4 AAK69605 Sli AAF37559 Hpu APN2 AAK58066 Hvi AAC36148 Pin AAX39865 Tni APN3 AAF01259 Pxy APN3 Q11000 ... order Lepidoptera to be fully characterized and sequenced. 0.1 AAB70755 Pxy AAX39863 Tni APN1 AAF08254 Hvi AAN75693 Har APN1 AAF37558 Hpu APN1 AAC33301 Bmo Q11001 Mse A pisum APN CAA66467 Pxy...
Ngày tải lên: 16/03/2014, 12:20
Báo cáo khoa học: Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis docx
... 2387 Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis Puja Shahi* † , Ish Kumar* ‡ , Ritu Sharma, Shefali Sanger and Ravinder S. Jolly Institute of Microbial ... prevent the abnormal accumulation of intracellular acyl-CoA. We have isolated a thioesterase from Alcaligenes faecalis ISH108 and demonstrated its application in chemoselectiv...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
... 5’-GC TCTAGAATCTACAAACCTAAAACAAC-3’ XbaI stkP deletion DFKRP 5’-TGC CCGCGGTCATAATATCACGGACCGCAT-3’ SacII stkP deletion CAT1 5’-CGC GGATCCGAAAATTTGTTTGATTTTTAA-3’ BamHI stkP replacement CAT2 5’-GC TCTAGAAAGTACAGTCGGCATTAT-3’ ... Purpose STKP-F 5’-AGGATGC CATATGATCCAAATCGGCAA-3’ NdeI stkP expression STKP-R 5’-TTGATTAT GAATTCGCTTTTAAGGAGTAGC-3’ EcoRI stkP expression STKP-RT 5’-GTAGGACA GAATTCAAG...
Ngày tải lên: 16/03/2014, 18:20