Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx

... consistent with the ADH4 activity previously found in this epithelium [25]. Thus, the presence in the RPE, the high activity with 11-cis-retinol, and the specificity for the oxidation direction of the ... ADH4 is detected in the retinal pigment epithelium (RPE) and it is extensively distributed in the retina. ADH4 is found in the outer (ONL) and...

Ngày tải lên: 23/03/2014, 12:20

... in the presence of 10 mm arabinose and made competent by con- centrating ten-fold and washing five times with ice-cold 10% glycerol. Selection for the recombination event and the elimination of ... found in the 23S rRNA variant containing the double mutation U1911C ⁄ U1915C (Fig. 3). Thus, isomerization of uri- dines 1911 and 1917 in H69 occurs autonomously of...

Ngày tải lên: 18/02/2014, 16:20

... together, the results of this study are consis- tent with the notion that residue 33 is involved in the interactions of the enzyme with the substrate in both UGT2B4 and UGT2B7. In contrast to the ... to hypothesize that the aromatic tyrosine residue at posi- tion 33 in UGT2B4 may play an important role in the substrate specificity of the isoform. Impor...

Ngày tải lên: 23/03/2014, 09:21

... persisted in investigating the in vivo specificity of the yeast HAT-B complex, and found that incubation of cells with hydroxyurea (HU) resulted in an increase of the histone H4 isoform with acetylated ... treatment. To investigate whether HU induces an increase of the Hat1 protein, we used a yeast strain expressing a hemagglutinin (HA)-tagged version of Hat1....

Ngày tải lên: 07/03/2014, 05:20

... University of California, Berkeley, USA). (C) Diagram showing the structure of human TMP kinase (1E2D.pdb), with the main motifs: the LID is in red, the P loop in blue and the adenine loop in black, with ... enzyme, the replacement of the conserved alanine into a tyro- sine in the binding site of thymidine, shifted the enzyme specificity towards guanos...

Ngày tải lên: 16/03/2014, 14:20

... C-1¢ of the xylosyl moiety and H-6 of the fructosyl moiety, and the other showed a corre- lation between C-1¢ of the xylosyl moiety and H-3 of the fructosyl moiety in the HMBC spectrum. This indi- cated ... [4]; however, the aglycone specificity of this enzyme has remained obscure. In this study, we characterized the aglycone-binding site of YicI by dete...

Ngày tải lên: 23/03/2014, 07:20

... Representation of hormone specificity, ligand-binding domains, transmembrane-spanning regions, intracellular domains and signaling systems of GC-A ⁄ NPRA, GC-B ⁄ NPRB, and NPRC. The arrows indicate the ligand ... variability in terms of their ligand specificity and signal transduction activity. Two of these receptors contain intrinsic GC activity, and have been design...

Ngày tải lên: 28/03/2014, 23:20

... cyclobrassinin and camalexin (0.10 and 0.30 mm final concentrations). The phyto- alexin that displayed an inhibitory effect was further examined in order to determine the type of inhibition and the ... For N H S N SCH 3 Cyclobrassinin A B C Fig. 8. Lineweaver–Burk plots of BH activities for (A) BHLmL2 and (B) BHAb in the presence of the phytoalexin cyclobrassinin. E...

Ngày tải lên: 18/02/2014, 14:20

... glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are mainly in the range ... different orientation of the substrate w ithin the substrate binding site of PAT2, and this may simultaneously avoid the interference of the polar side ch a...

Ngày tải lên: 07/03/2014, 16:20

... end of the active site cleft, which accounts for its exo mode of action. In contrast, XEG lacks a corresponding loop at this site, thereby allowing binding to the middle of the main chain of the ... domains, each consisting of a seven-bladed b-propeller. These are located tandemly in the N- and C-terminal halves of the poly- peptide, and are joined on each edge...

Ngày tải lên: 23/03/2014, 05:22