Báo cáo khoa học: S -Stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino acid amidase doc

... be a novel L -stereoselective amino acid amidase, LaaA. This is the first report revealing the primary structure of L -amino acid amidase. Materials and methods Bacterial strains, plasmids and ... endonucleases were purchased from Takara Shuzo and alkaline phosphatase from shrimp was purchased from Roche Diagnostics GmbH (Mannheim, Germany). Chromosomal DNA was prepared...

Ngày tải lên: 23/03/2014, 12:20

... LSSGSACSSYRTEASHVLYA 339 IscS A. Vin VSSGSACTSASLEPSYVLRA 34 1 IscS E.coli VSSGSACTSASLEPSYVLRA 34 1 IscS V.fis VSSGSACTSASLEPSYVLRA 356 A tNfsl Ara VSSGSACTSASLEPSYVLRA 262 N ifs Ana ASSGSACTSGSLEPSHVLRA ... of this analysis in terms of OsiS function is discussed below. IscR maturation in an iscS sufS mutant of E. coli expressing osiS To assess whether OsiS could be involved in the bio-...

Ngày tải lên: 18/02/2014, 04:20

... Oda 2 , Shigeyuki Imamura 1 and Masatake Ohnishi 2 1 Department Diagnostics Research and Development, Division of Fine Chemicals and Diagnostics, Asahi Kasei Pharma Corporation, Shizuoka, Japan; 2 Department ... liver 3a- HSD, the first HSD to be assigned to the AKRs, is involved in bile acid biosynthesis, and participates in the inactivation of circulating steroid hormones such as androge...

Ngày tải lên: 07/03/2014, 15:20

... We excluded the possibility that this was a consequence of enzyme denaturation by assaying the enzyme during incubation. A reasonable explanation is that NADPO was acting as a competitive inhibitor ... recombinant bovine AdR was a gener- ous gift of R Bernhardt (Universita ¨ t des Saarlandes, Saar- bru ¨ cken, Germany). NADP + , NADPH, NAD + and AMP were purchased from Sigma-Aldri...

Ngày tải lên: 16/03/2014, 11:20

... Michaelis–Menten constants of d-Phe-Pip-Arg-pNA (Instrumentation Labor- atory, Milan, Italy) hydrolysis, as well as the equilibrium dissociation constant of a- NAPAP (Sigma-Aldrich, St. Louis, ... that in the thrombin amidase cycle the His57 imidazolium form acts as a general acid to facilitate amine expulsion from the tetrahedral interme- diate, and this process is the rate-limiti...

Ngày tải lên: 07/03/2014, 12:20

... with previous findings that S- nitrosothiols play a central role in plant disease resistance [47]. NO levels have been associated with signaling in plant disease resis- tance [48]. Our data suggests that ... post-translational modification of PEPC by nitrosylation occurs in both Arabidopsis ,a C 3 plant [9], and K. pinnata, a CAM plant (this study). Carbonic anhydrase is present in anim...

Ngày tải lên: 16/03/2014, 06:20

... C-terminal fragments, phosphorylation, and glycosylation of amino -acid residues [13]. The latter is a remarkable characteristic of many archaeal and some bacterial S- layer proteins. The glycan chains ... comparison of protein sequence data and CD measurement of various S- layer proteins can be summarized as follows. There are large amounts of glutamic acid and aspartic acid ( 15...

Ngày tải lên: 16/03/2014, 12:20

... degradation of all stereoisomers. Thus, GC analysis was used to monitor the enzymatic hydro- lysis of all stereoisomers of cyclosarin, soman and IMP-pNP. The GC analysis demonstrates 50% degra- dation ... Michaelis–Menten kinetic analysis was per- formed with paraoxon and parathiol using some of the var- iants. In this case, product formation during hydrolysis of paraoxon and parathiol was me...

Ngày tải lên: 16/03/2014, 13:20

... namely Theta, Zeta, Phi, Tau and O mega [3,4,9]. Whereas Zeta, Theta and Omega classes of GSTs are found in plants and animals, the large Phi and Tau classes a re unique to plants [9]. In maize ... used for inactivation (pH6.5)wasthesameasthatnecessaryforhighGST activity. This probably affords an enzyme conformation similar to that adopted during catalysis, t hus creating more favourable co...

Ngày tải lên: 16/03/2014, 16:20

... period. Statistical significance was determined by analysis with Fisher&apos ;s exact test using MS Windows Statview (Abacus Con- cepts, Berkeley, CA, USA). P < 0.05 was considered statisti- cally significant. Results During ... emergency responses and teams. A traditional cardiac arrest ('code blue') team is comprised of a cardiology fellow and coronary care nurse, as wel...

Ngày tải lên: 25/10/2012, 10:45