Báo cáo khóa học: Metal-binding stoichiometry and selectivity of the copper chaperone CopZ from Enterococcus hirae pot

... with a change in the hydrophobicity of the localenvironment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of the protein. A plot of the intensity of the signal at ... solution of copper- loaded EhCopZas it may offer a molecular basis for the copper- transfermechanism from the copper chaperone to the targetprotein.Another relevant question is the selectivity of thesemetallochaperones ... http://blackwellpublishing.com/products/journals/suppmat/EJB/EJB4001/EJB4001sm.htmÓ FEBS 2004 EhCopZ metal binding and selectivity (Eur. J. Biochem. 271) 1003 Metal-binding stoichiometry and selectivity of the copper chaperone CopZ from Enterococcus hirae Agathe Urvoas1,...

Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot

... to otherproteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... the N-terminal, structured part of Xis is involved in the recognition of DNA [18,57]. On the other hand, the resultsalso indicate that the C-terminal a-helix, predicted in the work of Wu and co-authors for ... betweenb-strands 3 and 4. The b-strands 2 (residues Ile30–Phe31) and 5 (residues Val48–Lys49) form the second b-sheet. The Fig. 4. Identiﬁcation of the IsoAsp66–Gly67 connectivity. (A) The deamidation of...

Báo cáo khoa học: Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

... members of the bZIP family of transcription factors (Nrf2 and Maf G ⁄ K) bind [10]. The enzymes contain two bindingsites within the active site, a G-site for the binding of GSH and a H-site for the ... Using the standard curve constructed from the protein standards (Fig. 4B), the molecular mass of the Peak 1 GST subunit was determined to be 34.0 kDa(Table 2). Peak 2 showed 2 subunits of 36.8 and 32.6 ... [21]. The hallmarks of anoxia tolerance in turtles include a profoundlowering of metabolic rate and a buffering of lacticacidosis [22]. The magnitude of metabolic depressioncan be 10–20% of the...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... expression of T-bet has beenseen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... CD4+CD25+Foxp3+Tregor whetherthey induce the production of these cytokines from another population of cells [20].To date, our knowledge about the different types of Th and Tregresponses relates ... end of the gene, where the zebraﬁsh and human sequences appearto be quite diverse. Together with the well-conservedgene organization, there is a conservation of syntenybetween the human and...

Tài liệu Báo cáo khoa học: SREBPs: physiology and pathophysiology of the SREBP family ppt

... [20]. The role of SREBP-1a in the regulation of cell growth and the cell cycle might be biphasic and complex, and needs to be further investigated.SREBP is evolutionarily conserved; however, the key ... apparatus, where the site 1 and site 2 proteases cleave the SREBPs [3,4].Upon restoration of cellular cholesterol, Insig, anotherkey regulator of ER membrane proteins, traps and retains the SREBP–SCAP ... conditions of overnutrition,SREBP-1c expression is elevated, and consequently, the levels of nuclear SREBP-1c protein and lipogenesisare enhanced in the liver and adipose tissues. Intake of energy...

Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc

... analysis of the topological modules of the Fig. 3(labelled A–G) shows that they include structural and ⁄or functional features. Table 3 summarizes the main structural and functional features of thesegroups. ... functions, and topologi-cal features retain functionality and phylogeny. How-ever, the nature of the connections between thesefactors needs to be understood at the level of the pro-tein domain. The ... the mechanisms that shape the struc-ture of HTFN, we studied its topological modularityin relation to the function and structure of TFs from available information. From a structural point of...

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... The Authors Journal compilation ª 2009 FEBS of Spa¨tzle-C108 (Fig. 8A). Analysis of tryptic peptides from these bands by MS ⁄ MS and mascot softwareidentiﬁed them as attacin-1, lysozyme, and ... proHP8Xa and the catalytic domain of active HP8 are marked with arrowheads. The size and position of molecular weight stan-dards are indicated on the left. (B) The catalytic activity of activated ... proSpa¨tzle-1A is an ortho-log of the product of this gene. In the clade includingSpa¨tzle-1, the branch lengths are noticeably longer and the bootstrap values are lower than in the other cladescontaining...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... content in their latex; and (2) the pres-ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the proteinconcentration per milligram of dried latex, ... the other sequences. The MP tree reveals threemajor clusters. Cluster I combines the ﬁve chymo-papain isoforms of C. papaya with VXH-C, VS-B,CC-III and CC23. These results conﬁrm the hypothesis of ... Fractionation and puriﬁcation of the enzymes stored in the latex of Carica papaya.J Chrom B 790, 229–238.32 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophage...

Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx

... regulate the biosynthesis of the lipopeptides. The experiments performed in the present study showedthat this AAT can form Glu and Asp, and the forma-tion of Glu and Asp is used to synthesize Gln and Asn, ... determine the exact role of three residues in AATB3. The loss of the activity from the mutations together with the multiplealignment analysis indicated that the Asp232 residue of AATB3 enhances the ... active site and stabi-lizes the protonated N(1) of the co-enzyme tostrengthen the electron-withdrawing capacity of the co-enzyme [15]. The active site Lys258 transfers aproton from the amino...

Báo cáo khoa học: Identiﬁcation and expression of the ﬁrst nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt

... in the brain and further displays anumber of structural features not present in the othermembers of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of ... in the appearance of the APLP1 and APLP2gene families. The fact that both mammals and Xenopuscontain APP and APLP2 proteins suggests that the ﬁrstgene duplication giving rise to the APP and ... analysis of the APP superfamilyTo reveal the evolutionary history of the APP superfamily,we used maximum likelihood and Bayesian methods toconstruct a phylogenetic tree of the members of the APPsuperfamily...

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