Báo cáo khóa học: Metal-binding stoichiometry and selectivity of the copper chaperone CopZ from Enterococcus hirae pot

... with a change in the hydrophobicity of the local environment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of the protein. A plot of the intensity of the signal at ... solution of copper- loaded EhCopZ as it may offer a molecular basis for the copper- transfer mechanism from the copper chaperone to the target pro...

Ngày tải lên: 23/03/2014, 12:20

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... the N-terminal, structured part of Xis is involved in the recognition of DNA [18,57]. On the other hand, the results also indicate that the C-terminal a-helix, pre...

Ngày tải lên: 23/03/2014, 15:21

... members of the bZIP family of transcription factors (Nrf2 and Maf G ⁄ K) bind [10]. The enzymes contain two binding sites within the active site, a G-site for the binding of GSH and a H-site for the ... Using the standard curve constructed from the protein standards (Fig. 4B), the molecular mass of the Peak 1 GST subunit was determined to be 34.0 kDa (Table 2)....

Ngày tải lên: 23/03/2014, 15:20

... expression of T-bet has been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... CD4 + CD25 + Foxp3 + T reg or whether they induce the production of these cytokines from another population of cells [20]. To date, our knowledge about the differen...

Ngày tải lên: 16/02/2014, 09:20

... [20]. The role of SREBP-1a in the regulation of cell growth and the cell cycle might be biphasic and complex, and needs to be further investigated. SREBP is evolutionarily conserved; however, the key ... apparatus, where the site 1 and site 2 proteases cleave the SREBPs [3,4]. Upon restoration of cellular cholesterol, Insig, another key regulator of ER membrane pro...

Ngày tải lên: 18/02/2014, 13:20

... analysis of the topological modules of the Fig. 3 (labelled A–G) shows that they include structural and ⁄or functional features. Table 3 summarizes the main structural and functional features of these groups. ... functions, and topologi- cal features retain functionality and phylogeny. How- ever, the nature of the connections between these factors needs to be underst...

Ngày tải lên: 19/02/2014, 07:20

... The Authors Journal compilation ª 2009 FEBS of Spa ¨ tzle-C108 (Fig. 8A). Analysis of tryptic peptides from these bands by MS ⁄ MS and mascot software identified them as attacin-1, lysozyme, and ... proHP8 Xa and the catalytic domain of active HP8 are marked with arrowheads. The size and position of molecular weight stan- dards are indicated on the left. (B) The cat...

Ngày tải lên: 06/03/2014, 09:22

... content in their latex; and (2) the pres- ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration per milligram of dried latex, ... the other sequences. The MP tree reveals three major clusters. Cluster I combines the five chymo- papain isoforms of C. papaya with VXH-C, VS-B, CC-III and CC23. These result...

Ngày tải lên: 07/03/2014, 11:20

... regulate the biosynthesis of the lipopeptides. The experiments performed in the present study showed that this AAT can form Glu and Asp, and the forma- tion of Glu and Asp is used to synthesize Gln and Asn, ... determine the exact role of three residues in AATB3. The loss of the activity from the mutations together with the multiple alignment analysis ind...

Ngày tải lên: 14/03/2014, 23:20

... in the brain and further displays a number of structural features not present in the other members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of ... in the appearance of the APLP1 and APLP2 gene families. The fact that both mammals and Xenopus contain APP and APLP2 proteins suggests that the first gene...

Ngày tải lên: 16/03/2014, 16:20