Báo cáo khóa học: Active site residues and mechanism of UDP-glucose dehydrogenase ppt
... Active site residues and mechanism of UDP-glucose dehydrogenase Xue Ge 1 , Lisa C. Penney 1 , Ivo van de Rijn 2 and Martin E. Tanner 1 1 Department of Chemistry, University of British ... and aldehyde dehydrogenases [7]. Other members of this family include UDP-ManNAc dehydrogenase [8] and GDP-mannose dehydrogenase [9,10]. Extensive studies on both the bovine...
Ngày tải lên: 23/03/2014, 12:20
... that Arg206, Asn210 and Ala276 are important for the second-half reaction and are part of the active- site of CoaB; these residues may be directly involved in binding of one of the substrates, ... another site of CoaB and that exchanges of the residues Arg206, Asn210 or Ala276 indirectly influence the binding of cysteine to CoaB complexed with 4¢-phosphopantothenoy...
Ngày tải lên: 19/02/2014, 12:20
... Woodward C (1998) Anat- omy of protein pockets and cavities: measurement of binding site geometry and implications for ligand design. Protein Sci 7, 1884–1897. Active site residues in aloesone synthase ... starter substrate, number of polyketide chain elongations, and mechanisms of the final cyclization ⁄ aromatization reactions. It has been demonstrated that the shape a...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Amyloid oligomers: formation and toxicity of Ab oligomers ppt
... characteristics of Ab oligomers and their formation mechanism remain unclear. Structure and size of soluble Ab oligomers Many types of natural and synthetic Ab oligomers of different sizes and shapes ... visualiza- tion of Ab oligomer at the molecular level in living cells and animal models, may be required to elucidate the mechanism of formation of Ab oligomers. P...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Active-site-specific chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx
... DGJ and the active- site of a- Gal A. DGJ is a known active- site directed competitive inhibitor of a-Gal A. Interactions of a-Gal A with DGJ were modeled based upon the crystal structure of a-Gal ... for Gaucher disease [20,21], Tay-Sachs and Sandhoff dis- ease [22] (details for Gaucher and Tay-Sachs ⁄ Sandhoff diseases are reviewed separately), GM1-gangliosidosis [23],...
Ngày tải lên: 30/03/2014, 03:20
Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx
... alternate cysteine residues (I-III, II-IV). The numbers of amino acid residues encompassed by the second and third cysteine residues (m) and the third and fourth cysteine residues (n) are the ... the mechanisms of incorporation of PTMs in the biosynthesis of conopeptides. Appropriate methods of analysis need to be addressed to ensure recognition of these PTMs, if pre...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... N-terminal and b-he lical domains participate in binding of the polysaccharide, pullulan. Comparison of the active sites of the model of IPU and Dex49A The active site structures of t he model of IPU ... sequence (residues 1–19) and a mature part (residues 20–564), and the model is composed of residues 25–540. The overall structure of the model of IPU and...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Active site residue involvement in monoamine or diamine oxidation catalysed by pea seedling amine oxidase doc
... Docking of substrates in the PSAO active site. Carbons of the substrate are shown in yellow and carbons of PSAO are shown in green. Residues that were mobile in docking simulations and residues ... the active site (before the chemical step). At pH 6, the total negative charge of the active site is reduced (perhaps one negatively charged group is pro- tonated) and...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase pot
... to perform site- directed mutagenesis of the residues N28, I29, F114 and Q81 in the Dm-dNK active site. The aim of the study was in part to understand the determinants of the substrate specificity of the ... Fujix). Results We have performed site- directed mutagenesis of four active site amino acid residues of Dm-dNK. The residues are N28, I29, Q81 and F114 that...
Ngày tải lên: 08/03/2014, 02:21
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... modifications of amino acid residues and identification of headspace volatiles, a chemical mechanism for hydro- lysis of the dithiocarbamate group of brassinin catalysed by BHLmL2 and BHAb is proposed. ... absence of divalent metal cations in the active site of BHs. Both enzymes were inhibited by 1.0 mm of 2-mercaptoethanol and dithiothreitol. For example, 1mm dithio...
Ngày tải lên: 18/02/2014, 14:20