Báo cáo khóa học: Active site residues and mechanism of UDP-glucose dehydrogenase ppt

... Active site residues and mechanism of UDP-glucose dehydrogenase Xue Ge1, Lisa C. Penney1, Ivo van de Rijn2 and Martin E. Tanner11Department of Chemistry, University of British ... and aldehyde dehydrogenases [7]. Other members of thisfamily include UDP-ManNAc dehydrogenase [8] and GDP-mannose dehydrogenase [9,10]. Extensive studies onboth the bovine and S. pyogenes UDP-glucose ... [6,6-di-2H]-D-glucose usingÓ FEBS 2003 Active site residues of UDP-glucose dehydrogenase (Eur. J. Biochem. 271)17hexokinase, phosphoglucomutase, UDP-glucose pyro-phosphorylase, and inorganic pyrophosphatase....

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... that Arg206, Asn210 and Ala276are important for the second-half reaction and are part of the active- site of CoaB; these residues may be directlyinvolved in binding of one of the substrates, ... another site of CoaB and that exchanges of the residues Arg206, Asn210 or Ala276 indirectly inﬂuencethe binding of cysteine to CoaB complexed with4¢-phosphopantothenoyl-CMP.Copuriﬁcation of the ... is also cofactor of enzymes that catalyze the biosynthesis of polypeptideantibiotics [1]. Lipmann discovered and characterizedcoenzyme A [2] and Lynen elucidated that the thiolgroup of the cysteamine...

Báo cáo khoa học: Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase docx

... Woodward C (1998) Anat-omy of protein pockets and cavities: measurement of binding site geometry and implications for liganddesign. Protein Sci 7, 1884–1897. Active site residues in aloesone synthase ... startersubstrate, number of polyketide chain elongations, and mechanisms of the ﬁnal cyclization ⁄ aromatizationreactions. It has been demonstrated that the shape and volume of the active site cavity greatly ... acetyl-CoA and four molecules of malonyl-CoA by PCS, and (E) SEK4 and SEK4b from acetyl-CoA and seven molecules of malonyl-CoA by OKS. Here BNY and CTAL are derailment by-products of the CHS...

Báo cáo khoa học: Amyloid oligomers: formation and toxicity of Ab oligomers ppt

... characteristics of Ab oligomers and their formation mechanism remain unclear.Structure and size of soluble AboligomersMany types of natural and synthetic Ab oligomers of different sizes and shapes ... visualiza-tion of Ab oligomer at the molecular level in livingcells and animal models, may be required to elucidatethe mechanism of formation of Ab oligomers.Possible mechanism of solubleoligomer ... complexity of the oligomer formation mechanism. The mechanisms of formation may also differ for extracellular and intracellular oligomers. In this section, we discuss pos-sible formation mechanisms of...

Báo cáo khoa học: Active-site-speciﬁc chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx

... DGJ and the active- site of a-Gal A. DGJ is a known active- site directed competitive inhibitor of a-Gal A. Interactions of a-Gal A with DGJ were modeled basedupon the crystal structure of a-Gal ... forGaucher disease [20,21], Tay-Sachs and Sandhoff dis-ease [22] (details for Gaucher and Tay-Sachs ⁄ Sandhoffdiseases are reviewed separately), GM1-gangliosidosis[23], and retinitis pigmentosa 17 [24]. ... Domain 1 contains the active- site atthe center of the b strands in the (b ⁄ a)8barrel, whereas domain 2contains antiparallel b strands. The galactose ligand is shown inyellow and red. (B) Fabry...

Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx

... alternate cysteine residues (I-III, II-IV).The numbers of amino acid residues encompassed by thesecond and third cysteine residues (m) and the third and fourth cysteine residues (n) are the ... themechanisms of incorporation of PTMs in the biosynthesis of conopeptides. Appropriate methods of analysis needto be addressed to ensure recognition of these PTMs,if present, in the course of ... characterization of nativea-conotoxins.Analysis of neuronally active a-conotoxinsusing HPLC and MS, including identiﬁcation of post-translational modiﬁcationsIsolation and identiﬁcationStandard procedures...

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of the active sites of the model of IPU and Dex49AThe active site structures of t he model of IPU ... sequence (residues 1–19) and a mature part (residues 20–564), and the model iscomposed of residues 25–540. The overall structure of themodel of IPU and the mutated residues in this study areshown ... in subsite )1.The r esidues that form potential subsites ) 1and) 2are reportedly more conserved in GH family 49 than the residues in s ubsites +1 and +2 [17]. Comparison of the active sites o...

Báo cáo khoa học: Active site residue involvement in monoamine or diamine oxidation catalysed by pea seedling amine oxidase doc

... Docking of substrates in the PSAO active site. Carbons of the substrate are shown in yellow and carbons of PSAO are shown ingreen. Residues that were mobile in docking simulations and residues ... the active site (before the chemical step). AtpH 6, the total negative charge of the active site isreduced (perhaps one negatively charged group is pro-tonated) and the slope of the plot of ... approach of Briggs and Haldane, kcis included in Km(Km=(k)1+ kc) ⁄ k1): hence, the independence of the Km of HEX from pH and that of the Km of CAD from tem-perature, and the strong...

Báo cáo khoa học: Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase pot

... toperform site- directed mutagenesis of the residues N28, I29,F114 and Q81 in the Dm-dNK active site. The aim of thestudy was in part to understand the determinants of thesubstrate speciﬁcity of the ... Fujix).ResultsWe have performed site- directed mutagenesis of four active site amino acid residues of Dm-dNK. The residues are N28,I29, Q81 and F114 that according to primary and tertiarystructural ... further understanding of the catalytic mechanism of the enzyme and the properties determiningthe broad substrate speciﬁcity of the enzyme. Although themaximal rate of purine and pyrimidine...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... modiﬁcations of amino acid residues and identiﬁcation of headspace volatiles, a chemical mechanism for hydro-lysis of the dithiocarbamate group of brassinin catalysed by BHLmL2 and BHAb is proposed. ... absence of divalent metal cations in the active site of BHs. Both enzymes were inhibited by 1.0 mm of 2-mercaptoethanol and dithiothreitol. For example,1mm dithiothreitol caused 72% and 85% ... 85% inhibition of BHLmL2 and BHAb, respectively, while 1 mm 2-mer-captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively.The inﬂuence of pH on the activities of the BHenzymes...

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