Báo cáo khoa học: Silencing the constitutive active transcription factor CREB by the LKB1-SIK signaling cascade pot
Báo cáo khoa học: Silencing the constitutive active transcription factor CREB by the LKB1-SIK signaling cascade pot
... http://www.blackwell-synergy.com Silencing of CREB by LKB1-SIK Y. Katoh et al. 2748 FEBS Journal 273 (2006) 2730–2748 ª 2006 The Authors Journal compilation ª 2006 FEBS Silencing the constitutive active transcription factor ... SIK1 One of the downstream branches of the SIK -signaling cascade leads to the regulation of CRE-dependent Y. Katoh et al. Silencing o...
Ngày tải lên: 23/03/2014, 11:20
Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx
... bound to the column. Again, to avoid irreversible aggregation of huhepc, the huhepc solution was directly lyophilized in the presence of buffer to maintain further solubility of the peptide. The peptide ... whether the hepcidins were stored at 4 °Corat)80 °C and in the presence or absence of glycerol or detergents. These polymeric hepcidin forms could not be reverted to the mo...
Ngày tải lên: 18/02/2014, 18:20
Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... in the affinity for GDP of Ga and the subsequent substitu- tion of GDP by GTP [10]. Such an agonist-dependent decrease in the affinity for GDP can be detected as the increase in binding of the ... Ga o [31,32]. The five regions of the Ga-subunit involved in receptor recognition are the a2 helix, the b6 ⁄ a5 loop, the a5 helix and the N- and C- extreme termini [31]. In add...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Distinguishing between different pathways of bilayer disruption by the related antimicrobial peptides cecropin B, B1 and B3 pptx
... based on the different thermotrophic phase behaviours and the shapes of the thermal phase- transition curves obtained. The kinetics of the lysis of giant unilamellar vesicles observed by microscopy ... sample loss caused by the adsorption of hydrophobic molecules to the surfaces of the flow system of the BIAcore instrument. Peptides dissolved in HBS-EP buffer are not lysed...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Nitric oxide-induced epidermal growth factor-dependent phosphorylations in A431 tumour cells pot
... part for the NO • -mediated inhibition of cell proliferation mediated by the cGMP-dependent pathway in smooth muscle cells [6]. On the other hand, the concomitant inhibition of both the ribonucleotide ... added growth factors (results not shown). Therefore, the addition of EGF does not increase the level of ERK1/2 phosphory- lation. In contrast, DEA-NO somewhat decreases the...
Ngày tải lên: 17/03/2014, 03:20
Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... further than other ribotoxins to become smaller and more economical. At the same time, the active site of HtA, as revealed by the 3D structure, shows a different arrangement to that shown by the ... connected by a hinge region. Further- more, the nature and location of the catalytic residues as well as the enzymatic mechanism (they are cyclizing RNases) are also conserve...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... for the chemotherapeutic treatment of cancer and viral diseases because they catalyse the addition of the first phosphate group to the nucleoside analogue. This primes the nucleoside for further ... in hydrogen bonding. The result that E52Q did not show any activity must therefore be a consequence of the reactivity of the functional group and further support the hypothesis th...
Ngày tải lên: 19/02/2014, 02:20
Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... substrate into the active site. At this point, a plausible explanation is that the substrate may enter by an alternative route (i.e. the back channel at the bottom of active site). This hypothesis is ... in the absence of inhibitors, we used the specific equation in Scheme 1. The effect of two ligands on the activity of the wild-type DmAChE was evaluated by the equa...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis pptx
... catalytic Asp to the active site. Each Asp is present in the hallmark motif Asp-Thr ⁄Ser-Gly, followed further downstream by a hydrophobic-hydrophobic-Gly sequence. Together, these motifs form ... segment is present in the shewasin A amino acid sequence. The Asp of the active site motif from the N-terminal domain (marked with an aster- isk) was mutated to an Ala to generate...
Ngày tải lên: 14/03/2014, 22:20
Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx
... compartment of the cell (e.g. mitochondria). The reason why the action of the two opposite enzymes, namely the arachidonoyl-CoA synthetase and the arachidonoyl-CoA thioesterase, is needed may be the need ... 8-Br- cAMP-induced steroid synthesis. These results together with our finding that AA or 22(R)-OH-cholesterol can bypass the effect of siRNA strongly indicate that ACS4 and...
Ngày tải lên: 16/03/2014, 18:20