Báo cáo khoa học: Characterization of testis-speciﬁc serine–

Báo cáo khoa học: Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc

... al. Characterization and regulation of TSSK3 Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase- 1-dependent signalling Marta Bucko-Justyna 1 *, ... phosphorylation of GST–TSSK3 with [ 32 P]ATP[cP]. The phosphorylation reactions of GST–TSSK3 K39R by Myc-PDK1 [32 ] immunoprecipitated from 2...

Ngày tải lên : 23/03/2014, 11:20

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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redeﬁnition of the I-conotoxin superfamily pot

... presence of reduced and oxidized glutathione with a yield of 70%, and purified to homo- geneity by RP-HPLC. The identities of the peptides were confirmed by MALDI MS, and the molecular masses of the ... the middle, and the other at one end, of the trough. Each pair of recording electrodes was connected to a differential A ⁄ C amplifier, and the signal bandpass-filtered (...

Ngày tải lên : 16/03/2014, 22:20

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Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx

... 30 Gal(b1-O)pNP 38 44 30 37 34 Glc(a1-O)pNP 22 62 28 31 28 Man(a1-O)pNP 29 205 22 59 29 Fuc(a1-O)pNP 31 58 23 40 31 Xyl(a1-O)pNP 35 64 24 37 31 a Anti-FLAG-beads bound lysate of Sf9 cells. b The ... CG8708 CG 139 04-1 CG2975 GalNAc(a1-O)Bz 36 27 415 107 170 182 GalNAc(b1-O)pNP 30 1126 30 39 37 GlcNAc(a1-O)pNP 24 14 426 55 120 32 GlcNAc(b1-O)pNP 25 76 21 35 27 Gal(a1...

Ngày tải lên : 23/03/2014, 15:20

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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx

... 186–190 and 233 –240 in b1, and 18–24, 30 38 , 98–107, 110–116 and 175–180 in b2. b2 is ﬂanked by two long antiparallel a helices ( 43 61 and 67–91). b1 is ﬂanked by an a-helical region comprised of ... of Asp36, Asp192 and Asp 231 were used. Alignment of 12 atoms resulted in an rmsd value of 0 .3 A ˚ . As shown, the modeled position of the phosphate from HsSTP is cl...

Ngày tải lên : 07/03/2014, 09:20

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Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx

... contact activation system. Initiation of activation of the system has been found to be a result of a slow autodigestion and autoactivation of surface bound FXII by FXIIa [2]. Binding of FXII ... protected from inactivation by C1-INH by a factor of 3 10. Comparison of the rate constant for inactivation of FXIIa in the medium with that previously published for inact...

Ngày tải lên : 23/03/2014, 20:22

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Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf

... 1.01 b,t,g – -66 147 -33 -1 63 59 0.00 0.22 b,t,g + -68 118 -31 -161 -68 0. 63 1 .34 b,g + ,t - 75 162 30 - 93 172 2. 53 0.69 b,g + ,g – )75 158 25 )102 61 3. 41 1.47 b,g + ,g + )74 158 24 )102 )68 3. 38 1.76 c,g + ,t ... synthesis. (Received 26 February 20 03, revised 29 April 20 03, accepted 13 May 20 03) Eur. J. Biochem. 270, 2869–2878 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -...

Ngày tải lên : 08/03/2014, 02:21

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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc

... 4.589* H2 3. 917* 3. 930 * H3 3. 7 43 3.745 H4 3. 988* 4.002* H5 3. 967* 3. 976* H6 4.24 4.24 H6¢ 4.24 4.24 CH 3 2.056* 2.056* GlcA (B) H1 4.628* 4.556* H2 3. 480* 3. 451* H3 3. 638 3. 633 * H4 3. 77 3. 75 H5 ... 4.205 4.214 H6¢ 3. 811 4. 238 4. 236 CH3 2.1 13* 2.080* 2.0 73* GlcA-ol (A) H1 3. 644* 3. 679* 3. 672* H1¢ 3. 724* 3. 762* 3. 758 H2 3. 9 03 3.922* 3. 91...

Ngày tải lên : 16/03/2014, 14:20

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Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx

... AF104220. (Received 23 July 2002, revised 3 October 2002, accepted 14 November 2002) Eur. J. Biochem. 270, 84–92 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 033 64.x the expense of c-tocopherol ... on a Prontosil 200 3- C30-column (Bischoff Chromatography; NC; 230 · 4.6 mm, 3. 0 lm) by ﬂuorescence detection (Jasco FP-920 detector; k ex : 295 nm and k em : 33 2 nm). El...

Ngày tải lên : 17/03/2014, 09:20

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Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel speciﬁcity towards C-terminal glutamate residues pptx

... pro-region ( 13 kDa polypeptide). After 5 min the majority, and by 20 min all, of the original 50 kDa protein had been digested, with an increase in staining of the bands of  36 kDa and  13 kDa. On ... three polypeptides, a major band at  35 kDa, and a closely spaced doublet of bands at  30 kDa. Proteins were identiﬁed by N-terminal sequencing of polypeptide bands...

Ngày tải lên : 23/03/2014, 12:20

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Báo cáo khoa học: Characterization of novel sequence motifs within N- and C-terminal extensions of p26, a small heat shock protein from Artemia franciscana potx

... mass Maximum monomer number Range (kDa) Maximum (kDa) E. coli G 23. 7 14.2–4 43 4 43 19 R 24.1 14.2 30 0 30 0 12 TS 23. 8 14.2 30 0 30 0 13 WT 25.5 29.0–669 669 26 COS-1 cells G 18.7 14.2–4 43 4 43 24 R 19.4 14.2 30 0 30 0 16 TS 19.1 14.2 30 0 30 0 16 WT 20.8 ... Art p26 25°C 43 C p26 Bac R 31 0 1600 1400 1200 1000 800 600 400 200 0 25 A B 20 15 10 5 0 31 6 32 2 32 8 33 4 34...

Ngày tải lên : 30/03/2014, 20:20

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