Báo cáo khoa học: Extraenzymatic functions of the dipeptidyl peptidase IV-related proteins DP8 and DP9 in cell adhesion, migration and apoptosis doc

... DEC1 with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for ... to those of intact BMAL1 and DEC1, respectively (Fig. 1A,C). These findings indicate that the region between amino acids 28 and 139 of DEC1 (including the bHLH dom...

Ngày tải lên: 19/02/2014, 16:20

... Insulin-like growth factors and their binding proteins: biological actions. Endocr Rev 16, 3–34. 2 Firth SM & Baxter RC (2002) Cellular actions of the insulin growth factor binding proteins. ... fragments, have IGF-independent biological activities in cell adhe- sion and migration and in the regulation of the cell cycle and apoptosis [1–4]. Furthermore, alt...

Ngày tải lên: 07/03/2014, 00:20

... structures determine the unique functions of these flavin proteins? In this study, we present the crystal structure of DvMF flavoredoxin and discuss the key residues for ligand binding and metal ion binding, ... flavin-binding domain of ferre- doxin : NADP + reductase [10]. The lysine residue is not conserved; instead, an arginine residue interacts with the FAD molecule...

Ngày tải lên: 07/03/2014, 02:20

... an independent metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the ... residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in th...

Ngày tải lên: 14/02/2014, 22:20

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... ribonucle- otide binding and radical transfer from the tyrosyl rad- ical (Y122) of the R2 subunit to the cysteine (C439) found in the active site of t...

Ngày tải lên: 15/02/2014, 01:20

... in the A chain, and residues in green are in the B chain. (C) Vacant Thr-binding site in TtAKb- free. Residues in blue are in the E chain, and residues in orange are in the F chain. (D) Structure-based ... on the map. The electron densities of the sections (residues 54–56 in chains A and D, residues 53–59 in chain B, residues 55–56 in chain C, resid...

Ngày tải lên: 18/02/2014, 08:20

... components, including DNA, ssRNA, dsRNA and glycoproteins. This response initiates signaling pathways leading to the induction of protective cellular genes, including type-I interferons [initially interferon ... IFN-b through the phosphorylation of multiple cellular factors, including IFN regulatory fac- tor-3 (IRF-3) and kinases, including the Tank-binding kinase 1 (TBK-1) and...

Ngày tải lên: 18/02/2014, 16:20

... is the average of two independent measurements and has a standard deviation < 15%. The solid line represents the log-linear increase in the data set from pH of 5–7 (slope = 0.5 ± 0.03). The ... to bind the product relative to the ribozyme binding the substrate. This is perhaps due to destabilization of substrate binding via positioning of the 3¢-bridg- ing pho...

Ngày tải lên: 18/02/2014, 18:20

... cystein-rich (SRCR) domain, which was rigidly bound to the back of the proteinase domain in the crevice between the C-terminal helix, the 204-loop and the 126-loop [7]. The C-terminus of hepsin ... N-terminal SEA domain and the back site of the proteinase domain. This interaction would fix the SEA domain in a loca- tion on the opposite side of the protei...

Ngày tải lên: 19/02/2014, 00:20

... role in the enzymatic reaction which will be discussed later. The structure of the active site, the type of protein ligands, the zinc-binding motif, the presence of a water Fig. 3. Structure of the ... residue in the zinc- bound MshB structure (PDB code 1Q74) adopts a sim- ilar strained conformation. On the other hand, in the absence of a zinc ion in the...

Ngày tải lên: 19/02/2014, 00:20