Báo cáo khoa học: Functional characterization of hepatocyte nuclear factor-4a dimerization interface mutants pot

... 1948–1958 ª 2006 The Authors Journal compilation ª 2006 FEBS Functional characterization of hepatocyte nuclear factor-4a dimerization interface mutants Eleni Aggelidou*, Panagiota Iordanidou*, Constantinos ... Exclusive homodimerization of the orphan recep- tor hepatocyte nuclear factor 4 defines a new subclass of nuclear receptors. Mol Cell Biol 15, 5131–5143. E. A...

Ngày tải lên: 23/03/2014, 10:21

... in the course of the reaction. The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c. This was vali- dated by adding a total of 2.5 U of commercial SOD ... The assistance of T. Pavkov (Institute of Chemistry, University of Graz) in the acquisition of CD and DLS data is gratefully acknowl- edged. B. Kuczewski and H. Wiltsche (Instit...

Ngày tải lên: 18/02/2014, 06:20

... proteins as front-end D4 desaturases. Functional characterization of front-end desaturases Functional characterization was carried out by deter- mining the FA profiles of Saccharomyces cerevisiae transformed ... function of this class of enzymes merely on the basis of sequence homology. Moreover, they indicate that a com- plete pathway for very-long-chain polyunsaturated fatt...

Ngày tải lên: 07/03/2014, 12:20

... within negatively stained particles of artemin indicated the lack of metal storage capacity. Artemin, apoferritin and ferritin inhibit citrate synthase denaturation Artemin, apoferritin and ferritin protected ... with a broad substrate range, or by a com- bination of these capabilities. Moreover, the results expand the potential protective role of apoferritin ⁄ ferritin in stressed ce...

Ngày tải lên: 16/03/2014, 11:20

... 1.647 nm (confidence interval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively. These ... compared to those of bipartite switch GE + V:Hs-LmRXR. The major advantages of these new versions of VGEtvy + Hs-LmRXR and VGEtvay + Hs-LmRXR switches are the following:...

Ngày tải lên: 16/03/2014, 12:20

... amplitude of the fast phase of the l atter represen ted up to 40% of the total amplitude, with a rate constant (k obs ) independent of Pc concentration (data not shown). From the k obs values of this fast ... electrostatic poten- tial of fern Pc drastically hinders its interaction with spinach PSI. In order to extend t he function al characterization of fern Pc, we have also c...

Ngày tải lên: 16/03/2014, 16:20

... regions of this eIF2a kinase are required for its appropriate subcellular localization of DPERK. Thus, in the absence of each one of these two sequences, the majority of the respective DPERK mutants moved ... forms of Myc- tagged derivatives in Drosophila S2 cells, as indicated in Fig. 7. Expression and activity of DPERK mutants. (A) Autokinase and eIF2a kinase activities of...

Ngày tải lên: 31/03/2014, 07:20

... (1990) Reg- ulation of the maltose transport system of Escherichia coli by the glucose-specific enzyme III of the phosphoenolpyruvate-sugar phosphotransferase system. Characterization of inducer exclusion- resistant ... of MalFGK 2 (Eur. J. Biochem. 269) 4085 Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of...

Ngày tải lên: 31/03/2014, 09:20

... the analysis of the metabolic control of flux through the Leloir pathway. Analysis of the galactokinase, its mutants, and the other enzymes of the metabolic pathway using the principles of a quantitative ... committed step in the catabolism of galactose. The sugar is phosphor- ylated at position 1 at the expense of ATP. Lack of fully functional galactokinase is one cause of...

Ngày tải lên: 08/03/2014, 02:20

... amino-acid sequence of the artemin monomer consists of 230 residues with a calculated molecular mass of 25 976 Da. The ferritin cDNA (accession number AY062897) of 725 bp consists of an ORF of 516 bp, ... regulation of artemin and Artemia ferritin mRNAs Probing of Northern blots with a labeled artemin probe revealed bands of  2.1 and 3.7 kb, the former of expected length...

Ngày tải lên: 23/03/2014, 20:22