Báo cáo khoa học: Analyses of co-operative transitions inPlasmodium falciparumb-ketoacyl acyl carrier protein reductase upon co-factor and acyl carrier protein binding pdf

... retinoid binding, and x-ray analysis of a human retinol -binding protein. Proc Natl Acad Sci USA 98, 3710–3715. 5 Folli C, Calderone V, Ramazzina I, Zanotti G & Berni R (2002) Ligand binding and ... retinol -binding proteins (CRBPs) and the cel- lular retinoic acid -binding proteins (CRABPs). CRBPs, including CRBPI, CRBPII, CRBPIII and CRBPIV, show greatest binding affin...

Ngày tải lên: 16/03/2014, 22:20

... Plasmodium falciparum b-ketoacyl acyl carrier protein reductase upon co-factor and acyl carrier protein binding Krishanpal Karmodiya and Namita Surolia Molecular Biology and Genetics Unit, Jawaharlal ... Plasmodium b-ketoacyl- acyl carrier protein reductase exhibits negative, homotropic co-operative binding for NADPH, which is enhanced in the presence of...

Ngày tải lên: 23/03/2014, 10:21

... appearance of a number of novel antigen -binding specificities (Fig. 1B). Enhanced binding of low-pH buffer-exposed IVIg to recombinant human interferon-c (IFN-c) and other self-antigens Interactions of ... detectable binding to CRP at the concentration and during the period of observation used in the experiment. Interaction analyses using increasing concentrations of th...

Ngày tải lên: 16/02/2014, 15:20

... for Protein Analyses and Targeted Protein Research Program of the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), by Core Research for Evolutional Science and ... system. Fig. 1. Comparison of 1 H- 13 C constant-time HSQC NMR spectra of 0.6 mM of UL At3g16450.1 and 0.2 mM of SAIL At3g16450.1. (A) Full spectrum of UL At3g16450.1. (B...

Ngày tải lên: 18/02/2014, 14:20

... amounts of protein for purification. We also attempted to isolate the protein from the E. coli periplasm but could not find any evi- dence of activity, indicating a lack of export of the protein. ... cysteine-histi- dine thioether bridge in functional units of molluscan haemocyanins and location of the disulfide bridges in functional units d and g of the bC-haemocyanin of...

Ngày tải lên: 06/03/2014, 11:20

... Howard S. Young 3 and Kunio S. Misono 1 1 Department of Biochemistry, University of Nevada, Reno, NV, USA 2 Department of Chemistry, University of Nevada, Reno, NV, USA 3 Department of Biochemistry, ... fluorescence studies of Trp residues, taking advantage of the fact that Trp74 occurs at the hh interface and that its local environ- ment changes upon ANP binding, whereas...

Ngày tải lên: 07/03/2014, 03:20

... Site-directed mutational analysis for the ATP binding of DnaA pro- tein. Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP -binding domain of DnaA protein in vitro and in vivo. J Biol Chem ... Mg 2+ and ATP concentrations during electropho- retic separation, may influence binding, and we think this the more likely explanation; direct comparison...

Ngày tải lên: 07/03/2014, 09:20

... pGal4-CREB (1–280), pCRE-luc containing four tandem repeats of the CREB- binding sites, pGal4-luc containing five tandem repeats of Gal4 -binding sites, and the mammalian expression vector pCMV-PKAc ... conditions and luciferase assay Transient transfection of Jurkat T-lymphocytes was accomplished by using 5 lL of SuperFect transfection reagent (Qiagen) per ml of culture mediu...

Ngày tải lên: 08/03/2014, 10:20

... stabiliza- tion of labile drugs, control of volatility and sublimat- ion, physical isolation of incompatible compounds, long-term protection of color, odor and flavor, and sup- pression of hemolysis and the ... pockets of a-CD and b-CD are not able to form stable complexes with GA, the binding of a-CD to GA is not observed and that of b-CD is much weaker than that...

Ngày tải lên: 16/03/2014, 14:20

... were expressed and purified by protein A affinity chroma- tography, and IgG in yields of 0.5–3 mgÆL )1 were obtained. Biacore analysis of binding kinetics revealed that the affinities of the IgGs derived ... highest-affinity and lowest-affinity IgGs were determined to have K D values of 1 and 440 nm, respectively. Enrichment of high-affinity and moderate-affinity IgGs To eval...

Ngày tải lên: 22/03/2014, 21:21