Báo cáo khoa học: Deoxyribonuclease I footprinting reveals different DNA binding modes of bifunctional platinum complexes potx

... mole- cules of biological significance [1,6–15], including antitumor cis-diamminedichloroplatinum(II) (cisplatin) (Fig. 1B) and its clinically ineffective trans isomer [16]. The bifunctional platinum complexes, ... high sensitivity of DNase I to DNA conformational alterations induced by platinum com- plexes so that the platinum adducts which induce specific local conforma- tion...

Ngày tải lên: 23/03/2014, 10:21

... recombinant x-5 gliadin protein that can be used for diagnosis and possibly treatment of WDEIA. Results Identification of IgE -binding epitopes in x-5 gliadin The IgE -binding epitopes of x-5 gliadin ... patients. The binding of IgE to nOG5 was completely inhibited by increasing amounts of rOG5C in all patients. At an inhibitor concentration of 0.1 and 1 lgÆmL )1 , nOG5 inhibit...

Ngày tải lên: 20/02/2014, 01:20

... that folding of G-rich oligonucleotides into quadruplex structures proceeds via kinetically significant interme- diates. These intermediates may consist of antiparallel double hairpins in rapid equilibrium ... Coupling of local folding to site-specific binding of proteins to DNA. Science 263, 777–784. 50 Lane AN & Jenkins TC (2000) Thermodynamics of nucleic acids and their int...

Ngày tải lên: 06/03/2014, 09:22

... the aromatic pyrimidine ring and the indole group of Trp27 should to be weaker in comparison with the purinetrione inhibitors due to the smaller size of the pyrimidine ring. It has in addition resulted ... active site with an inhibitor molecule of proper size, shape and atomic composition clearly stabilizes the interac- tion of the subunits within the pentamer. This conclu- sion is in...

Ngày tải lên: 07/03/2014, 11:20

... was established with its N-terminal 66 residues. Binding of Golga3 increases d-serine synthesis. Intriguingly, this is achieved through a decrease in ubiquitin ⁄ proteasomal degradation of serine racemase, ... of calcium, it is possible that some other GRIP-interacting protein that also binds to PDZ6 might disrupt the serine racemase–GRIP interaction, hence diminishing the activity o...

Ngày tải lên: 30/03/2014, 04:20

... its 72 kDa progelatinase complex. Demonstration of the biochemical similarities of tissue inhibitor of metalloproteinases-2 and tissue inhibitor of metalloproteinases-1. Biochem J 278, 179–187. 16 ... cysteine- rich protein with kazal motifs (RECK) is a cell mem- brane associated inhibitor of MMP-2 that is able to inhibit the second activation step of MMP-2 [9]. The small calcium...

Ngày tải lên: 18/02/2014, 11:20

... all fish examined had significant levels of protein in blood and skin during the winter. It is possible that different phy- siological or environmental cues initiate expression in each tissue separately. ... as important for ice binding [21–23]. Recently, amino acid substitution experiments have determined that it is the conserved Ala-rich hydrophobic surface which is most important for i...

Ngày tải lên: 20/02/2014, 02:22

... expression. The stem-loops and palindromes constructed by inverted repeats are involved in RNA interference, transcription initiation of genes, initiation of DNA replication and alternative splicing ... cruciforms: significance for initiation of DNA replication. J Cell Biochem 63, 1–22. 25 McAlinden A, Havlioglu N, Liang L, Davies SR & Sandell LJ (2005) Alternative splicing of...

Ngày tải lên: 07/03/2014, 00:20

... gene families in rice. In this study, whole genome microarray analysis of auxin-responsive genes in rice was performed, with the aim of gaining some insight into the mechanism of auxin action. A ... positively regulate the expression of downstream genes involved in auxin signaling. The differential and overlapping expression patterns of individual members of these gene families in...

Ngày tải lên: 07/03/2014, 01:20

... compilation ª 2009 FEBS Metal-induced impairment of mitochondrial function J. Ciapaite et al. Modular kinetic analysis reveals differences in Cd 2+ and Cu 2+ ion-induced impairment of oxidative ... quantification of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72, 248–254. 40 Ciapaite J, Van Eikenhorst G, Bakker SJ, Diamant M, Heine...

Ngày tải lên: 16/03/2014, 02:20