Báo cáo khoa học: Characterization of mutations in crucial residues around the Qo binding site of the cytochrome bc1 complex from Paracoccus denitrificans pdf

... monitoring of the protonation state of several crucial residues in the presence and absence of stigmatellin. Interestingly, several residues perturb the orientation of the quinone binding site ... 2008) doi:10.1111/j.1742-4658.2008.06611.x The protonation state of residues around the Q o binding site of the cyto- chrome bc 1 complex from Para...

Ngày tải lên: 23/03/2014, 10:20

... likely that the binding of subunit 3 is a necessary prerequisite for the binding of critical, nuclear-encoded subunits that stabilize the growing com- plex. Thus, while the binding of subunit ... to that of the bovine enzyme, the structure of the bovine oxidase can be used to predict some of the conse- quences of the human mutations. Most of the mut...

Ngày tải lên: 23/03/2014, 21:20

... pattern matching and manual supervision procedure. With these seed sets they then performed the following iterative method to enlarge the initial training sets, adding examples to both classes from an ... irrelevant keywords in the majority of cases. 2.3 Feature (or keyword) selection To handle the inherent noise in the training dataset that originates from its weakly supe...

Ngày tải lên: 31/03/2014, 00:20

... influ- ential interaction between the interviewer and the inter- viewed during the interviews that could influence the statements of the interviewed e.g., the use of leading ques- tions. In the phase of ... advisors These are veterinarians who primarily followed their own definitions of different scoring values, such as excluding certain scores (see examples in table...

Ngày tải lên: 25/10/2012, 10:45

... protein developed for measuring [Ca 2+ ] within the mitochond- rial matrix and was instrumental in gaining new insight into mitochondrial Ca 2+ handling, i.e. the topic of this review [13]. In the ... aequorin moiety including an HA1 tag was fused in frame with the N-terminal portion (including the 25 amino acids cleavable presequence and the first eight amino acids of t...

Ngày tải lên: 30/03/2014, 20:20

... readily detected in association with PS1 complex in embryonic tissue as indicated by reactivity of NCT ectodomain antibody. NCT was barely detectable in the PS complex from adult mouse brain as shown in the ... shown by others [5,17]. The complex was stable in 0.5% DDM even after addition of reducing agent (1% b-mercaptoethanol), indicating strong interaction, inde...

Ngày tải lên: 16/03/2014, 16:20

... Fig. 1B (insert), which shows the characteristic cross peak from the geminal protons of carbons 1 and 3 of glycerol in TG [1]. Cross peaks of lipids, including the T signal, were absent in cells ... compared in samples obtained by cells harvested at different days from the same seeding. In the following, ML quantification derives from intensity measurements of...

Ngày tải lên: 18/02/2014, 13:20

... was based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence. Cloning and sequencing of this fragment revealed ... of the sole Glyco_18 domain, the C-terminal tail of C. gigas CLPs may not noticeably contribute to the structure and the function of these protei...

Ngày tải lên: 19/02/2014, 00:20

... related proteins, as shown in Fig. 1B. To evaluate whether the Sp1 -binding site in the DNASE1 distal promoter is crucial for expression, a mutated binding site was introduced into the )197H construct, resulting ... of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step o...

Ngày tải lên: 19/02/2014, 06:20

... Glutamine was effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active site or the glutamine -binding site ... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open reading frame was deleted to result in a frame-shift and appearance of stop codons in...

Ngày tải lên: 19/02/2014, 12:20