Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264 7 murine macrophages pdf

... 73 Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264. 7 murine macrophages Sonia Scarfı ` 1,2 , Umberto Benatti 2 , Marina Pozzolini 1,2 , Emanuela Clavarino 2 , Chiara Ferraris 1,2 , Mirko ... untreated quartz (75 4.2 pgÆmL )1 versus 272 .9 pgÆmL )1 ). Time-course of COX-2 mRNA synthesis in RAW 264. 7 macrophages COX-2 mRNA s...

Ngày tải lên: 23/03/2014, 10:20

... consisting of an agent doing the abridging and an optional theme that is being abridged. Furthermore, in an English sentence using the verbabridge, the agent is typically found in the subject ... each individual sense, a process that we could not do automatically. 3 Default Linguistic Information If information about a word is not found in any of our linguistic resources, the...

Ngày tải lên: 20/02/2014, 18:20

... against the Ca 2+ concentration [annexin A1 in (B), annexin A2 in (C), annexin A5 in (D), annexin A1 + annex- in A2 + annexin A5 in (E)]. Data points represent mean ± standard error determined in ... annexin A1, annexin A2 and an- nexin A5 show Ca 2+ -dependent binding to dicalcin, as the 38 kDa and the 35 kDa proteins do. For this, we obtained recombinant annexin A1, annexin A2 a...

Ngày tải lên: 07/03/2014, 05:20

... phosphatases. J Biol Chem 277 , 49808–49814. 33 Higashino K, Otani R, Kudo S & Yamamura Y (1 977 ) A fetal intestinal-type alkaline phosphatase in hepatocel- lular carcinoma tissue. Clin Chem 23, 1615–1623. 34 ... Conversion of a magnesium binding site into a zinc binding site by a single amino acid substitution in Escherichia coli alka- line phosphatase. J Biol Chem 268, 214 97 21500...

Ngày tải lên: 07/03/2014, 17:20

... phosphate-buffered saline (NaCl/P i ) containing 0 .5% bovine s erum albumin (BSA), washed 3 · with NaCl/P i and incubated in anti-LSR serum (1 : 10 in NaCl/P i /BSA) for 2 h at 37 °C. The sections were washe d in ... targeting of the LSR gene and generation of LSR deficient mice The murine (C57BL/6) LSR gene contains 10 coding exons with an open reading frame of 178 2 nuclotide long...

Ngày tải lên: 16/03/2014, 18:20

... M5a1 into pBSK + vector creating pRS1 67, followed by insertion of thetetracyclineresistancecassetteintotheEcoRV site of fdnG fragment resulting in pRS 177 . The 3.2-kb EcoRI/KpnI fragment of pRS 177 ... pRS191 carrying the interrupted nuoCD region was transferred into the allelic exchange vector, pKAS46 [ 27] creating plasmid, pRS1 97; (d) pRS1 97 was transformed into RAS46 and recombinan...

Ngày tải lên: 17/03/2014, 10:20

... but also in identifying several of them as ouabain or its isomer [3–6] or as its congeners, such as digoxin [7, 8], proscillaridin A [9], 19-norbufalin [10] and marinobufagenin [11]. In addition, ... 2; Worthing- ton) in Pucks saline solution (Seromed, Berlin, Germany) containing 20 m M Hepes, and incubated at 37 °Cfor 20 min to detach the endothelium. The cells were washed out using 2...

Ngày tải lên: 30/03/2014, 13:20

... · 10 7 cellsÆmL )1 , and processed according to published procedures [9,16,22], with some modifica- tions. Total lysates were obtained by boiling 10 min in phosphate-buffered saline containing ... [ 27] , including Fuc-TVII that is not expressed in any cell line used in the present study [ 27 29]. However, moderate amounts of sLe x were recently proved to be the most efficient in prom...

Ngày tải lên: 19/02/2014, 12:20

... ovarian carcinoma; Mdm2, murine double minute 2; MMC, mitomycin C; NF-jB, nuclear factor-jB RNAi, RNA interference; siRNA, small interfering RNA. 1 470 FEBS Journal 278 (2011) 1 470 –1483 ª 2011 The Authors ... significant increase in the expression of the cyclin-dependent kinase inhibitor p21, a target of p53 [10]. Comprehensive gene expression profiling has revealed that the pattern o...

Ngày tải lên: 22/03/2014, 16:20

... Am.J.Clin.Nutr. 67, 1210–1218. 7. Fischer-Nielsen, A., Loft, A. & Jensen, K.G. (1993) Effect of ascorbate and 5-aminosalicylic acid on light-induced 8-hydroxy- deoxyguanosine formation in V79 Chinese ... of 5 m M ascorbic acid. Ó FEBS 2003 Ascorbic acid induces conformational change in DNA (Eur. J. Biochem. 270 ) 3103 (TEM) image of T4 DNA in the presence of 5 m M ascorbic aci...

Ngày tải lên: 23/03/2014, 21:20