Báo cáo khoa học: Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc

... Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction Maarten Hoogenkamp 1 , ... binding. Discussion Transcriptional regulation results from the cooper- ative binding of transcription factors [12]. One of the key determinants of the activation...

Ngày tải lên: 23/03/2014, 10:20

... PAGE of the resulting pellets. (C) The amounts of I C in the pellets. The amounts of I C were quantitated with the UN-SCAN-IT gel program (Silk Scientific Corporation, Orem, UT) using the band of purified ... site of I C overlap, and that the N-ter- minal segment at the CPY-binding sites participates in regulation of the specific binding of I C to the anionic...

Ngày tải lên: 19/02/2014, 05:20

... relation- ship between the conformation of the D-loop (ordered or disordered) and the state of the nucleotide-binding cleft (open or closed) [13]. These data are consistent with the results of the biochemical ... cleft; removal of the nucleotide or cat- ion results in actin denaturation. Therefore, the stabil- ity of actin depends on the affinity of the tightly...

Ngày tải lên: 06/03/2014, 22:21

... demonstrated that the cleavage could take place at multiple sites. We observed that the main digestion of the urease by HpU-9-L was initiated by the cleavage of the peptide bond at E124-G125 of the ... concentration. At 4 h of incubation (lane 2), significant changes of the band pattern were observed. The bands of partially dissociated urease (a m b n ) became...

Ngày tải lên: 07/03/2014, 21:20

... (see the absence of the C73 c-phosphate binding fragment in lane 5) [20], but the application of an ATP regeneration system restored the usual ATP cleavage pattern (data not shown). The C-terminal ... character- istic of the c-phosphate (C73 and N39-42) appear neither at the N- nor the C-terminal site, respectively. Instead, the 39- kDa fragment present at the C-...

Ngày tải lên: 08/03/2014, 02:21

... located immedi- ately adjacent to the N-terminal signal peptide [7,14– 17]. The propeptide mediates binding of the substrate to the carboxylase and also activates the enzyme. The discovery of ... Post-transalational modification Gla(3)–TxVI: confirmation of the presence of BrTrp. Product ion mass spectrum of the doubly charged ion at m ⁄ z ¼ 660.18. The isotopic di...

Ngày tải lên: 23/03/2014, 11:20

... a transcription factor inducible by multiple stimuli to regulate a wide range of genes. Involvement of the NF-jB signaling pathway in the regulation of genes involved in spermatogenesis and other ... with the CCAAT ⁄ enhancer binding protein beta (C ⁄ EBPb) to regulate the expression of Nur77, a regulator of steroidogenic-enzyme genes [18,20]. In investigating activation...

Ngày tải lên: 29/03/2014, 00:20

... type of cellular activation, including mitochondria hyperpolarization and the for- mation of the immunoproteasome. When considering the importance of the induction of cell death in cancer treatment, ... analysis On the basis of these results, and to better define the therapeutic potential of IFN-c in the treatment of as- troglioma cells, the antiproliferative ac...

Ngày tải lên: 29/03/2014, 22:21

... pegylated interferon alfa and ribavirin therapy and the HCV genotype should be systematically determined before treatment, as it determines the indication, the duration of treatment, the dose of ... enzyme that catalyzes the transformation of a substrate into a colored compound. The optical density (OD) ratio of the reaction (sample OD/internal control OD) is propor...

Ngày tải lên: 02/11/2012, 09:56

... solubilization by the enzymes is greatly hampered upon modification of the SBS. At the same enzyme concentration, the wild-type XBS solubilized the most. The maximal attainable sol- ubilization of these ... investigating the structure of carbohydrate- active enzymes have revealed the presence of other substrate binding regions situated on the surface of the st...

Ngày tải lên: 14/02/2014, 19:20