Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique Juana ... influence the efficacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homo...

Ngày tải lên: 23/03/2014, 10:20

... of the 53 kDa spot (5.3) was identical to a pI value calcu- lated from the amino acid sequence of GmPDIL-3a, and the isoelectric point of the 57 kDa spot (5.1) was consistent with that from the ... organelle and membrane proteins are synthe- sized and folded with the assistance of molecular chap- erones and other folding factors in the endoplasmic reticulum...

Ngày tải lên: 18/02/2014, 11:20

... cloned and characterized the cDNAs of GmPDIL-1 and GmPDIL-2 as members of the PDI family. The amino acid sequences and putative domain structures of GmPDIL-1 and GmPDIL-2 were similar to each other, ... b-conglycinin during the course of the folding process of these proteins. Results Cloning and expression of GmPDIL-1 and GmPDIL-2 To clone the soybean ort...

Ngày tải lên: 07/03/2014, 05:20

... parameters of CYP719A3, the amount of nandinine produced was estima- ted by using the calibration curve of (S)-scoulerine (pmol versus peak area) and the absorption ratio of (S)-scoulerine and (S)-nandinine ... prepared from coptisine by the same manner as (R,S)-tetrahydroberberine. Amplification of cytochrome P450 cDNA fragments from single-stranded cDNAs of...

Ngày tải lên: 07/03/2014, 10:20

... strains B, Z85 and BL21(DE3) and bacteriophage T5 + were taken from the collection of the Laboratory of Molecular Microbiology of the Institute of Biochemistry and Physiology of Microorganisms ... Triton X-100), and the chelators were added. The reaction was ini- tiated by the addition of a standard amount of the enzyme (15 ng mL )1 ). Extraction of...

Ngày tải lên: 30/03/2014, 02:20

... [15], suggesting the existence of additional OSCs. As part of an effort to understand the molecular mechanism of the triterpene cyclization reactions, as well as the molecular evolution of these proteins ... mL·min 21 . The contents of bryonolic acid and total sterols were calculated from the peak area of their trimethylsilylated products relative to that of...

Ngày tải lên: 08/03/2014, 23:20

... expression of T-bet has been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... CD4 + CD25 + Foxp3 + T reg or whether they induce the production of these cytokines from another population of cells [20]. To date, our knowledge about the differen...

Ngày tải lên: 16/02/2014, 09:20

... 40G14, 9E14, 51H3, and 6H19. The BAC DNA was isolated, and the presence of the SmSmad1B sequence was confirmed by PCR and by sequencing the BAC DNA. Exon–intron sites were located by aligning the cDNA sequence ... R-Smads (Smads 1, 5 and 8), as the functions of these Smads in other organisms are highly redundant. Future analysis of the target genes activat...

Ngày tải lên: 18/02/2014, 16:20

... physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of these two coenzymes. Also potassium trithionate and sodium ... amounts of th e 16-kDa c-type cytochrome. The Fig. 2. Genomic o rganization of the genes e ncoding the subunits of the Hme complex from A. f...

Ngày tải lên: 21/02/2014, 03:20

... content in their latex; and (2) the pres- ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the protein concentration per milligram of dried latex, ... research was provided by a grant to T. Kyndt from the ‘Institute for the Promo- tion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)’ and by...

Ngày tải lên: 07/03/2014, 11:20