... FAD bindingto the apo-D-AAO, using d-alanine (25 mm) as substrate.The rate of NADH oxidation in the l-lactate dehydro-genase-coupled reaction was followed spectrophoto-metrically at 340 nm ... postmitoc-hondrial supernatant. Postmitochondrialsupernatants (0.1–0.2 mg) were collectedfrom either intact (I-mt SN), digitonin-solubi-lized (D-mt SN) or osmotically shocked (OS-mt SN) TBY-2 ... against the chaperonin (i.e. a-Cnp, a kind giftfrom C. Indiveri, Universita`della Calabria, Calabria, Italy).a-FAD- and a-Cnp-immunoreactive materials were visual-ized with the aid of a secondary...