Báo cáo khoa học: Activity of matrix metalloproteinase-9 against native collagen types I and III potx

... monomeric forms of native collagen types I and III at 37 °C and 25 °C, respectively. Activity against collagens I and III was abolished by metalloproteinase inhibitors and was not present in the concentrated ... demonstrating specific cleavage of native type III collagen with this enzyme (Fig. 4, type III, T) [27]. The more extensive digestion of denature...

Ngày tải lên: 23/03/2014, 09:21

... matrix binding properties of recombinant fibronectin type II-like modules of human 72-kDa gelatinase ⁄ type IV collagenase. High affinity binding to native type I collagen but not native type IV ... internalization and degradation. Role of exosites in regulation of activity The complex substrate specificity of individual MMPs is not only determined by their substrate-bind...

Ngày tải lên: 15/03/2014, 00:20

... zinc ion, peptidomimetis are subdivided into Table 2. Potential matrix metalloproteinase inhibitors. MMPI Type of drug ⁄ source Enzymes inhibited Synthetic inhibitors Batimastat Peptidomimetic ... generation of MMPIs, tetracycline derivatives, inhibit both the enzymatic activity and the synthesis of MMPs via blocking gene transcription. Chemically modified tetracyclines, lacking an...

Ngày tải lên: 15/03/2014, 00:20

... the similarity with animal insulin in binding the basic 7S globulin and stimulating its protein kinase activity [16], the 4 kDa soybean peptide was initially designated leginsulin [12] but this ... a cross-kingdom bioactive peptide family. Interestingly, similarity searches using the Swiss Institute of Bio- informatics (SIB) BLAST network service (http:// www.expasy.org/cgi-bin/blast.pl...

Ngày tải lên: 23/03/2014, 09:21

... used in this work are listed in Table 5. Split-ubiquitin technique For the split-ubiquitin interaction assays, 10 5 wild-type cells carrying the split-ubiquitin plasmids were spotted onto SC-His ... proximity. This reconstituted quasi -native ubiquitin is recognized by ubiquitin- specific proteases (USPs), which cleave off the reporter RUra3, which is fused to the PAK. Released RUra3, a modifi...

Ngày tải lên: 18/02/2014, 13:20

... Angelo Vianello 2 1 Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Universita ` di Trieste, Italy 2 Dipartimento di Biologia ed Economia Agro-Industriale, Sezione di Biologia Vegetale, ... bilirubin, biliverdin, section B) and in Table 2 (K d of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin 3-glucoside, sections C and D). A Michaeli...

Ngày tải lên: 20/02/2014, 01:20

... Choedchai Saehuan 2, * and Michael J. McLeish 1 1 Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, IN, USA 2 Department of Medicinal Chemistry, University ... when alternative pathways are avail- able, it is unlikely to play any significant role in the catabolism of the branched-chain amino acids or of methionine. Identification and...

Ngày tải lên: 06/03/2014, 00:21

... C-terminal region of GRIP1 is involved in the repression of transactivation activity and self- association of GRIP1 (Figs 1–5), we examined the relationship between transactivation and co-activation of ... 2183 Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-associat...

Ngày tải lên: 07/03/2014, 12:20

... xtracellular matrix could occur through the Fas I domains or putative GPI unit, if one exists in CLAP as it does in Drosophila fasciclin I and many other fasciclin-containing proteins [36,46]. Fas I domains in ... the dimer retained about 70% of its initial activity under these conditions (Fig. 4C). Similar differences in cathepsin L activity were observed at a ll incubation t...

Ngày tải lên: 07/03/2014, 16:20

... to virus infection induces sufficient apoptosis to limit the levels of virus multiplication, thus mimicking an antiviral effect. An alternative mechanism, that spermine acts by inhibition of virus entry, ... ArgII expression was IFN-independent (Fig. 3). ArgI and ornithine decarboxylase (ODC) are not induced in response to virus infection As the two isoforms of arginase, I (hepatic i...

Ngày tải lên: 07/03/2014, 21:20