Báo cáo khoa học: Activity of the plant peptide aglycin in mammalian systems potx

... aglycin binding protein purified by affinity chromatography. Lane A, protein molecular mass mark- ers; lane B, the aglycin binding protein isolated from mice; lane C, the aglycin binding protein ... originating from the animal kingdom which makes the present finding that the plant peptide aglycin binds to VDAC-1 in porcine pancreas even more interesting, in particular b...

Ngày tải lên: 23/03/2014, 09:21

... However, the presence of the tags in the loop make the membrane-inserted protein significantly more sensitive to trypsin, apparently by disrupting the interaction between the loop and the PSI core. Knock-out ... consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family. LHCI is bound along one side of PSI only, in the vicinity of the...

Ngày tải lên: 07/03/2014, 21:20

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the V. spinosum genome. ... likely histidine 80 in the V. spinosum tyrosinase. Another motif, which...

Ngày tải lên: 06/03/2014, 11:20

... Mn 2+ resulted in a restoration of the activity to the level of the k Mn obs of the unmodified enzyme (Table 1, Fig. 2B), implying a possible coordi- nation of the metal cation to one of the two (the proR P or ... occur in the presence of thiophilic cations such as Mn 2+ ,Zn 2+ ,orCd 2+ ,in increasing order (the rescue effect). Analysis of these types of...

Ngày tải lên: 07/03/2014, 10:20

... Examples of the linking rules Figure 1: The organization of the relations with te- linkage that this organization of the relations are viewed from the perspective of re-linkage. The different ... recognizing the coherence rela- tion between clauses is that the constituents of one clause bear certain kind of structural relationship to those of the other....

Ngày tải lên: 08/03/2014, 05:21

... apo- lipoprotein B/E-containing lipoproteins in the presence of free fatty acids, and is thought to be involved in the clearance of triglyceride-rich lipoproteins (TRL). The distribution of LSR in mice ... sections of livers of E14.5 em- bryos stained with hematoxylin and eosin (C,D,E,F). Note the presence of large inter- cellular spaces (arrow) in the liver of...

Ngày tải lên: 16/03/2014, 18:20

... pairing of the inter- chain disulfide bridges in the recombinant sample [11]. The kinetics of the N-terminal arms swapping in the P19A-BS- RNase is comparable to that of BS-RNase (Fig. 5B). Interestingly, ... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Carmine Ercole 1 , Francesca Avit...

Ngày tải lên: 16/03/2014, 23:20

... decrease in the levels of hDlg protein in the human epithelial cell line HeLa when exposed to osmotic shock. This is independent of the phosphorylation state of hDlg, is prevented by preincubating the ... diffusely throughout the cytoplasm in the vicinity of the mem- brane (Fig. 6Ca–c). However, pretreatment of the cell with the p38MAPK inhibitor BIRB0796 cau...

Ngày tải lên: 23/03/2014, 06:20

... anaerobically cultivated in phosphate Fig. 2. The mechanisms of the ring contraction process in the biosynthesis of vitamin B 12 postulated on the basis of the isolation of factor IV from the anaerobe P. ... of 13 C, 18 O-precorrin- 3x [8–10] as shown in the lower row of Fig. 1. We were interested in the mechanism of cor- rin formation by the anaerobe P...

Ngày tải lên: 23/03/2014, 09:20

... times using NaCl ⁄ P i and the target protein was eluted by incubating the beads in 1· SDS buffer for 15 min at 55 ° C or boiling the beads in 1· SDS gel loading buffer for 10 min. Following SDS ... that the putative RhlB-binding microdomain can bind RhlB. We then used Blue Native-PAGE, a technique commonly employed in the separation of membrane protein complexes, in a stu...

Ngày tải lên: 29/03/2014, 21:20