Báo cáo khoa học: Activity of the plant peptide aglycin in mammalian systems potx

... aglycin binding protein puriﬁedby afﬁnity chromatography. Lane A, protein molecular mass mark-ers; lane B, the aglycin binding protein isolated from mice; lane C, the aglycin binding protein ... originatingfrom the animal kingdom which makes the presentﬁnding that the plant peptide aglycin binds to VDAC-1 in porcine pancreas even more interesting, in particularbecause all cystine-knot ... emphasized that even though aglycin, likeinsulin, in uences the concentration of glucose in blood, the effect is opposite that of insulin, increasingrather than decreasing the blood glucose concentration.VDAC-1...

Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc

... However, the presence of the tags in the loopmake the membrane-inserted protein signiﬁcantly more sensitive to trypsin,apparently by disrupting the interaction between the loop and the PSI core.Knock-out ... consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family. LHCI isbound along one side of PSI only, in the vicinity of the PSI-F and PSI-J subunits [2,3]. Apart from the asymmetry ... conformation of the loop.His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding apopro-tein, Lhcb1 [18]. Interestingly, fusion of a His-tag to the C-terminus,...

Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase geneand surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the V. spinosum genome. ... likely histidine 80 in the V. spinosumtyrosinase. Another motif, which is present not only in tyrosinases, but also in the oxygen transportinghaemocyanin proteins, is the PYWDW (amino acids118–122) ... hypothesized to be involved in oxygen binding [26].Previous sequence analysis in other studies has dem-onstrated the presence of a conserved Yx(Y ⁄ F) motif in the C-terminal domains of both the...

Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx

... Mn2+resulted in a restoration of the activity to the level of the kMnobs of the unmodiﬁedenzyme (Table 1, Fig. 2B), implying a possible coordi-nation of the metal cation to one of the two (the proRPor ... occur in the presence of thiophilic cations such as Mn2+,Zn2+,orCd2+ ,in increasing order (the rescue effect). Analysis of thesetypes of interaction led to a better understanding of the mechanistic ... s, making kinetic analysisimpossible. Whereas the P8 substitution had only aminor effect both in the presence of Mg2+and in the presence of Mn2+, causing a 30–60% increase in krel,the...

Báo cáo khoa học: "Recognition of the Coherence Relation between Te-linked Clauses" potx

... Examples of the linking rules Figure 1: The organization of the relations with te- linkage that this organization of the relations are viewed from the perspective of re-linkage. The different ... recognizing the coherence rela- tion between clauses is that the constituents of one clause bear certain kind of structural relationship to those of the other. Although there are an infinite ... However, there ex- ists the constraint on the fitness with te-linkage as mentioned in the previous section. The explanation for the other relations is detailed in (Oishi, 1998). As a result of the...

Báo cáo khoa học: Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR–/– pdf

... apo-lipoprotein B/E-containing lipoproteins in the presence of free fatty acids, and is thought to be involved in the clearance of triglyceride-rich lipoproteins (TRL). The distribution of LSR in mice ... sections of livers of E14.5 em-bryos stained with hematoxylin and eosin(C,D,E,F). Note the presence of large inter-cellular spaces (arrow) in the liver of the mutant (C,E) contrasting with the ... kb of cloned genomic DNA sequence containing the 5¢-part of exon 2, intron 1, exon 1 and its 5¢-ﬂankingnoncoding sequence, and 2.7 kb of sequence containing the 3 ¢-part of exon 5 and 3¢-ﬂanking...

Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx

... pairing of the inter-chain disulﬁde bridges in the recombinant sample [11]. The kinetics of the N-terminal arms swapping in the P19A-BS-RNase is comparable to that of BS-RNase (Fig. 5B).Interestingly, ... Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNaseCarmine Ercole1, Francesca Avitabile1, ... have investigated the effects of mutation of two paradigmatic residues involved in the dislocation of BS-RNase N-terminal arms, i.e. Pro19 and Leu28,located in the hinge peptide and in the intersubunitinterface,...

Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot

... decrease in the levels of hDlg protein in the human epithelial cell line HeLa whenexposed to osmotic shock. This is independent of the phosphorylation state of hDlg, is prevented by preincubating the ... diffuselythroughout the cytoplasm in the vicinity of the mem-brane (Fig. 6Ca–c). However, pretreatment of the cellwith the p38MAPK inhibitor BIRB0796 causes a sig-niﬁcant delay in the loss of Dlg from the ... gain a better understanding of the role of hDlg phosphorylation in the adaptation of cells tostress, by determining whether stress could regulatehDlg degradation in cells exposed to changes in...

Báo cáo khoa học: Mechanism of the ring contraction process in vitamin B12 biosynthesis by the anaerobe Propionibacterium shermanii under aerobic conditions docx

... anaerobically cultivated in phosphateFig. 2. The mechanisms of the ring contraction process in the biosynthesis of vitamin B12postulated on the basis of the isolation of factor IVfrom the anaerobe P. ... of 13C,18O-precorrin-3x [8–10] as shown in the lower row of Fig. 1.We were interested in the mechanism of cor-rin formation by the anaerobe P. shermanii in anoxygen-containing atmosphere. Therefore, ... ratio of the 13C18O-carbonyl oxygen of [1-13C,1,1,4-18O3]ALA.Mechanism of the ring contraction process in the biosynthesis of vitamin B12by P. shermaniiTwo mechanisms of the ring contraction...

Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... times using NaCl ⁄ Piand the target protein was eluted by incubating the beads in 1·SDS buffer for 15 min at 55 ° C or boiling the beads in 1·SDS gel loading buffer for 10 min. Following SDS ... that the putative RhlB-binding microdomain can bindRhlB. We then used Blue Native-PAGE, a technique commonly employed in the separation of membrane protein complexes, in a study of the ﬁrst of its ... wepresent a model for the RNA degradosome complex in V. angustum S14 which shows the positions of the microdomains in the CTH of RNase E, the proteinsthat bind to them, as well as their dimerization...

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