Báo cáo khoa học: Expression and characterization of recombi

Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

... present in the sponge recombinant 2-5A synthetase preparations. RNA binding of the sponge recombinant 2-5A synthetase All known vertebrate 2-5A synthetases are known to be activated by their cofactor, ... The effect of various potential activators on the 2-5A syn- thesizing activity of the recombinant porcine 2-5A synthetase (A) and N-terminally His-tagged r...

Ngày tải lên : 23/03/2014, 09:20

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Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... increased by the addition of 2mm CoCl 2 (relative activity to that of the standard reaction 170%) and not by the addition of 2 mm ZnCl 2 or one of the other metals ⁄ salts tested. The enzyme was ... and New England Biolabs (Ipswich, MA, USA). Pfu Turbo and T4 DNA ligase were purchased from Invitrogen and Stratagene (Amsterdam, the Netherlands), respectively. For...

Ngày tải lên : 16/03/2014, 14:20

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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... experimentally. Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modiﬁcation of the catalytic properties of the catalytic histidine. ... located within the Ônucleophilic elbowÕ connecting strand b5 and helix a3, while His290 and Asp260 e merge from the b8-a8andb7-a7 loops, res...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Expression and characterization of the bioﬁlm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... without a loss of activity. For the characterization of metal ion effect, the 5¢-fusion of His . Tag and thrombin cleavage site sequence was cleaved from recombinant PepD protein. The recombinant ... role for these residues on the catalysis and zinc ions, respectively. These effects may be necessary either for the activation of the catalytic water molecule or f...

Ngày tải lên : 23/03/2014, 06:20

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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx

... exhibiting the bands of the remaining oxy complex. The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of broad band spreading 600–700 nm. The latter ... A. Addition of ascorbate to the heme–Syn HO-1 complex commences the reaction, which is monitored by the steady decrease of the Soret and 498 nm ban...

Ngày tải lên : 23/03/2014, 20:22

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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

... report, we present the expression and characterization of the ECDs of the b, c and e subunits of the human muscle AChR. We describe their expression in a soluble, glycosylated form and in satisfactory amounts ... on data from electron images of the AChR found in large amounts in the electric organ of the marine ray, Torpedo californica [3]. The acquis...

Ngày tải lên : 30/03/2014, 10:20

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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... harvested every 4 h and frozen in liquid nitrogen. The d-CDes mRNA levels increased at the end of the light period, reached a maximum at the end of the dark phase and decreased at the beginning of the light ... samples, and were used for the quantiﬁcation of the enzymatically formed H 2 S. Measurement of pyruvate formation The activity of the puriﬁed d-...

Ngày tải lên : 16/03/2014, 18:20

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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... 2002 by the addition of proFIX18 and proPT18, indicating that the Conus carboxylase, like the bovine carboxylase, is activated by the human propeptides. To compare the potency of the effect of these ... not shown). The difference in the expression level in the two systems is related to the efﬁciency of carboxylase expression in these systems. The speciﬁc ca...

Ngày tải lên : 17/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... effects, decreasing protein production and gene expression for IL-1b and TNF-a [11]. To address these paradoxes of both pro- and anti-inﬂammatory effects, the secretion of IL-1b and TNF-a from MSCs under ischaemic ... induces the transcription and secretion of IL-10 Because of the lack of secretion of the inﬂammatory cytokines IL-1b and TNF-a from hypoxia...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... comprising the two sugar binding pockets in the pulchellins (Fig. 3). In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-...

Ngày tải lên : 18/02/2014, 16:20

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