Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf

... FEBS Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum Deborah K. Shoemark 1 , Matthew J. Cliff 2 , Richard B. Sessions 1 and Anthony R. Clarke 1 1 Department of ... polarity of the environment of the fluorophore. In the case of PfLDH, the signal change on binding to the active site was too small to be used as a reliable r...

Ngày tải lên: 23/03/2014, 09:20

... tetramers from the hexamer was considered to result from direct action of the enzymes. On the other hand, the pentamer appeared to be formed by the condensation of smaller intermediates. Note that the ... (GlcNAc) 3 +(GlcNAc) 5 . The rates of forma- tion were in the order of (GlcNAc) 6 > (GlcNAc) 5 > (GlcNAc) 8 for both enzymes. The ratios of the maxima...

Ngày tải lên: 20/02/2014, 01:20

... and therefore the FRET assay was used to study the effect of pH on the enzyme. The stability of the synthetic SceD peptide substrate was determined by incubating it in different buffers in the ... using these assays. This line of research has the potential to result in a new class of Fig. 7. A comparison of the activities of the full-length and the truncated S...

Ngày tải lên: 07/03/2014, 01:20

... identified by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... and the equilibrium of self-association appears to control the biological properties of the protein [7]. Moreover, the immunomodulatory activity is related...

Ngày tải lên: 07/03/2014, 14:20

... changing the activity of the enzyme by x% results in a significant change of the pathway flux by at least 0.5x% (whether 0.5x% or higher is a matter of convention). The way that the change of enzyme ... is capable of reducing the level of fructose 2,6-P 2 inde- pendent of the phosphorylation state of iPFK-2. Reducing the level of fructose 2,6-P 2 and thus...

Ngày tải lên: 14/03/2014, 23:20

... con- served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impaired in an as yet unknown manner. Sequence alignments of the large GTP-binding domains of hGBP1, ... for the lack of GMP formation (see Fig. S2). The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are in contrast to that o...

Ngày tải lên: 15/03/2014, 10:20

... sites. Refolding of the scrambled RNase in the presence o f P DI was used as a positive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin from Alicyclobacillus ... wasusedasanegative control. The refolding of the scrambled RNase in the presence of Pf PDO seems to be less efficient when using PDI. However, the t...

Ngày tải lên: 16/03/2014, 16:20

... the superimpo- sition of ApeSOD with the cambialistic SOD from P. shermanii. The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was 0.695 A ˚ . ApeSOD eluted from ... ligands. The additional water occupied the postulated superoxide bind- ing site. The thermal stability of the enzyme was compared with superoxide dismutase from T...

Ngày tải lên: 14/02/2014, 22:20

... responsible for the change of the oligomeric state. These data point to an essential function of the small subunit of the decarboxylase and may indicate unique regulatory properties of the system. Keywords: ... allow in-frame cloning of the genes. While the BamHI sites introduced in the 3¢-UTR of the genes did not affect the resulting amino acid sequences, t...

Ngày tải lên: 07/03/2014, 15:20

... strain X-33 with the addition of a signal sequence for secretion of the recombinant pro- tein into the medium. Approximately 12 mg of the recombinant lectin was purified from the medium of a 1 L culture ... incomplete process- ing of the signal peptide was also reported [16,17]. The uncleaved part of the a-mating sequence at the N-ter- minus as well as the his...

Ngày tải lên: 14/03/2014, 23:20