Báo cáo khoa học: Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization pptx

... arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization Tsui-Yi ... translation inhibitory activity and subcellular localization. Results Phosphorylation of the RS-rich motif of the SARS-...

Ngày tải lên: 23/03/2014, 07:20

... Bud32p-depen- dent phosphorylation of other subunits of the complex could directly regulate its activity. In addition to the components of the EKC ⁄ KEOPS complex, many other proteins have been ... elongation. We have then examined the effects of Ser258 phosphorylation of Bud32p on the transcriptional activity of the EKC ⁄ KEOPS com- plex by analyzing the a...

Ngày tải lên: 07/03/2014, 04:20

... at neutral pH, affects the electrostatic potential and quite often the conformation of the modified protein. Even in the absence of rearrangement, the change in the electric field and steric hindrance ... tubulin-binding motif might offer some clues on its role in the progression of AD. The modulation of phosphorylation on aggregation might be essential for...

Ngày tải lên: 16/03/2014, 05:20

... 3–4), the phosphorylation was much weaker than that detected at 37 °C, supporting the idea that endocytosis of the receptor/ligand complex is important for the phosphorylation of Hrs. Nevertheless, ... though Hrs was identified as a phosphorylated protein, neither the responsible kinase(s), the exact phosphorylation site(s) nor the functional significance of the ph...

Ngày tải lên: 17/03/2014, 11:20

... 22-bedded general ICU. The sampling periods were 28 weeks before and 2, 10, 25 and 37 weeks after introduction of CPOE. The unit pharmacist prospectively recorded details of MEs and the total number of drugs ... committee. Prior to introduction of CPOE, local standards of prescribing existed specifying the tenets of good practice, including the avoid- ance of th...

Ngày tải lên: 25/10/2012, 10:39

... composed of a hOrn and a haOrn moiety. Furthermore, a long-range 1 H– 13 C correlation was detected between the carbonyl carbon of the serine and the d-CH 2 of the hOrn, which constitutes the DKP. Therefore, ... characterization of these compounds is growing and may result in the synthesis of specific inhibitors based on the structure of the pathogen sideropho...

Ngày tải lên: 16/02/2014, 09:20

... to study the binding of ATP alone to the protein were unsuccessful, as the protein precipitated upon addition of ATP in the absence of pantoate. An important feature of the NMR study is the fact ... molecule takes the position of the adenine ring of ATP. The carbon and oxygen atoms of pantoate are shown in yellow and red, respectively. The carbon and...

Ngày tải lên: 16/02/2014, 09:20

... to the relatively large number of studies devoted to the elucidation of the phosphorylation sites in HSL and the regulation of its translocation, few studies have addressed the effects of PKA phosphorylation ... to LPL in the vicinity of the active site and also to inhibit the enzymatic activity [12]. Simi- larly, we show here that bis-ANS inhibits the...

Ngày tải lên: 18/02/2014, 11:20

... a molecular mass of 16 309.7 Da, but this matched none of the DNA-predicted proteins. MS ⁄ MS of these ions gave the C-terminal sequence of Fig. 5. These and all other peaks of that spectrum ... accepted for the routine identification of proteins in complex mixtures. Usually, the identification of the gene that encodes the protein is more important than full struc-...

Ngày tải lên: 18/02/2014, 16:20

... and found that the cellular phosphorylation of CDK4 on Y17 is mediated by Src family kinases. Results Detection of CDK4 Y17 phosphorylation and kinase activity The N-termini of CDKs 1, 2, 4 and ... the appearance of kinase activity in those samples. The level of depletion of kinase activity mirrored the level of depletion of C-YES, indicating that C...

Ngày tải lên: 18/02/2014, 18:20