Báo cáo khoa học: Activation of PMCA by calmodulin or ethanol in plasma membrane vesicles from rat brain involves dissociation of the acetylated tubulin/PMCA complex pdf

... 2008 The Authors Journal compilation ª 2008 FEBS 3579 Activation of PMCA by calmodulin or ethanol in plasma membrane vesicles from rat brain involves dissociation of the acetylated tubulin /PMCA complex Noelia ... tubu- lin ⁄ PMCA complex. These results indicate that acetylated tubulin and PMCA form part of the same complex inserted into...

Ngày tải lên: 23/03/2014, 07:20

... Decrease in GDP affinity by carbamyl- choline. The experiment was performed in the presence of 1 m M carbamylcholine. Dotted line, in the presence of 0.2 mM atropine; solid line, in the absence of atropine. M. ... [ 3 H]QNB binding. The experiment was performed in the presence (dotted line) or absence (solid line) of 1 m M GTP. [ 3 H]QNB binding was normalized...

Ngày tải lên: 07/03/2014, 11:20

... caspase-3 activation by 30%; as did combined SB203580 and JNK inhibitor I, by about 70%; and the combination of SB203580, the JNK inhibitor I and a caspase-8 inhibitor, by 100%. These findings demonstrate the ... or PD98059), or combinations of inhibitors (SB203580 + JNK inhibitor I) or (SB203580 + JNK inhibitor I + caspase-8 inhibitor) or inhibitor-neg- ative contro...

Ngày tải lên: 07/03/2014, 12:20

... alternation of the n-6 ⁄ n-3 compositional ratio in favor of n-3 fatty acid results in a phosphorylation of Stat5, indicating a potential role of n-3 fatty acid in activating of Stat5 in the mammary ... preventative effect of a dietary supplement of n-3 PUFAs to the mother on the risk of breast cancer risk for offspring has been reported. Offspring of the...

Ngày tải lên: 16/03/2014, 10:20

... cleavage of the Arg407–Ile408 bond in response to tissue injury, generating the two-chain active form consisting of a disulfide-linked 66 kDa long chain and 32 kDa light chain [10,11]. The light chain ... involvement in the activation of HGF ⁄ SF in tumors [12–15]. Although thrombin is considered to be an activator of pro-HGFA [3,10], alternative pathways in the ac...

Ngày tải lên: 23/03/2014, 07:20

... previously reported that the specific activity of adsorbed enzyme (k) is greatly in uenced by the crys- talline polymorphic form of the substrate. Moreover, in the cases of cellulose I b from Halocynthia ... two- domain structures, with a catalytic domain (CD) and a cellulose-binding domain (CBD) [7–10]. As the ini- tial step of the reaction, they are adsorbed on th...

Ngày tải lên: 23/03/2014, 09:21

... fluorescein for acetylated and total a-tubulin and rhodamine for H + -ATPase. Fig. 4. Removal of acetylated tubulin from yeast membranes by alkaline treatment. Plasma membranes (5 mg of protein) ... 6.5, in the presence of various amounts of rat brain tubulin preparations containing low (d)orhigh(s) levels of the acetylated isotype (for details see the Experim...

Ngày tải lên: 23/03/2014, 11:20

... Studies of the binding of heparin oligosaccharides to well- known heparin-binding proteins, including the protease thrombin and fibroblast growth factor, have reported binding affinities (K D ) in the ... containing residues 1–26 away from the catalytic cleft and thereby increase the rate of activation. Whether GAGs directly increase the activity of proCD by weakenin...

Ngày tải lên: 30/03/2014, 02:20

... of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the activation domain [15]. This event is (part of) the ... important for TF binding and ⁄ or the cofactor effect [13]. In terms of intramolecular propagation of the TF-induced signal, the most interesting mutations ar...

Ngày tải lên: 18/02/2014, 08:20

... adenosine ring of Ade76. There is a general consensus that tRNA binding is not required for arginine binding [33], although arginine binding is a prerequisite for correct positioning of the CCA ... significantly. In contrast to l-canavanine, it is the bridging N of the guanidine group that is replaced by the heteroatom in l-thioarginine, locking the guanidino nitrogens in...

Ngày tải lên: 18/02/2014, 13:20