Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc

... splitting constants of the 15 N nucleus of the distal NO and of the 14 N nucleus of the proximal His of the GmHO-1 complex are closer to those of the rHO-1 complex than to those of the SynHO-1 complex. ... nm of each free hemin solution was measured, and these were plotted together with that of the corresponding hemin– protein solutions. The equi...

Ngày tải lên: 19/02/2014, 05:20

... heme-binding sites, it is unclear which heme contributes more to the binding of CO and the inhibi- tion of transcription. Also, the heme environment, including the axial ligands and heme-binding ... prepared the isolated domains by dena- turation of an insoluble suspension of E. coli cells overexpressing the protein and then subsequently refolding the protein in...

Ngày tải lên: 16/03/2014, 22:20

... of ligand binding of PGIS in solution and in sol–gel monolith In an attempt to stabilize the oxyferrous complex of PGIS for further studies, we adopted a method that immobilized the protein in ... Progression of ferrous–CO complex formation in sol–gel. Ferric PGIS (solid line), a ferrous–CO complex of 20 min of incuba- tion (dotted line) and a ferro...

Ngày tải lên: 23/03/2014, 07:20

... 1C). Increased nucleolin/C23 protein levels in Hsp70-transfected cells in response to H 2 O 2 -induced apoptosis We next examined the expression of nucleolin ⁄ C23 protein in these cells in response ... from the National Basic Research Program of China (2007CB512007), the National Natural Science Foundation of China (30700290) and Special Funds for PhD Training from The...

Ngày tải lên: 16/02/2014, 09:20

... TnC-bind- ing site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity of the troponin complex [17,18]. These ... probably induced by streng- thening of the interaction between the structural TnC- binding site and the C-domain of TnC accompanying Ca 2+ binding to site IV of...

Ngày tải lên: 20/02/2014, 01:20

... sentence The U.S. invaded Iraq in 2003.” 2 Low-level features As the foregoing implies, we are interested in train- ing a binary classifier, and so we represent each training and test instance in a feature ... E  s , with d x .d de- noting the index of the word that is the descendant in d x and d x .a denoting the ancestor. We define the following matching functio...

Ngày tải lên: 20/02/2014, 09:20

... drastic inhibition of translation in the RNA-injected cells [17]. Injection of neutralizing saporin antibodies in the host cell cytosol abolished the toxic effect, indicating that some of the newly ... toxin-free media (Fig. 2). The cells were then incubated for another 16 h in the absence of saporin and dimethylsulfoxide before determining the extent of intoxi...

Ngày tải lên: 07/03/2014, 05:20

... reduce the complexity of the hierarchical structure and reduce the number of levels in the hierarchy. This paper considers the tasks of identifying the syntactic structure of text chunking and gram- mar ... )))). where only the part -of- speech tags and grammar information are considered. The left hand side of the figure shows the original structure of...

Ngày tải lên: 08/03/2014, 02:21

... the 145 kDa form of GC-C and ligand responsiveness. Western blot analysis of cells cultured in the presence of swainsonine showed the presence of a band of 136 kDa, representing the partially glycosylated ... a reappearance of the 145 kDa form on the cell surface, fol- lowing processing of the endoplasmic reticulum-associated pool of the 130 kDa form. Selec...

Ngày tải lên: 08/03/2014, 08:20

... tilainen and Jiirvinen concern the (i) typology of the differences and (ii) the reliability of their ex- periment. Concerning the typology of the differences: in Voutilainen and J~irvinen's ... following table). 7. The remaining differences in the analyses were jointly examined by the linguists in or- der to see whether they were due to (i) inat-...

Ngày tải lên: 08/03/2014, 21:20