Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx

Báo cáo khoa học: A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase speciﬁc for b-alanyl dipeptides Hidenobu Komeda and Yasuhisa Asano docx

... 5¢-CACTTGAAGCTTTAAGGAGGAAtagACCATGCGTATCCGTGAGCTTGGCATCACC-3¢;antisen se primer, 5¢-ACGCAATCTAGAGTCAGCCCTCAGGGGGCTTTCG-3¢. The ampliﬁed PCR product wasdigested with HindIII and XbaI (Takara ... Experimen-tal procedures. Then a sample of the enzyme solutionwas taken, and the remaining activity of BapA wasassayed with d-Ala-pNA as a substrate under standardconditions. The enzyme was most stable ... L-Ala-(Gly)2(L-Ala)2, L-Ala-D-Ala, L-Ala-D-Ala-L-Ala,DL-Ala-DL-Asn, DL-Ala-DL-Ile, DL-Ala-DL-Leu, DL-Ala-DL-Met, DL-Ala-DL-Phe, DL-Ala-DL-Ser, DL-Ala-DL-Val, L-Asp-D-Ala, L-Pro-Gly, L-Pro-L-Phe, c-Aminobutyryl-L-His...

Báo cáo khoa học: A chromatin-associated protein from pea seeds preferentially binds histones H3 and H4 pptx

... acetylated and nonacet-ylated isoforms and H2B was not acetylated at all. The acetylation and AUT/PAGE analysis of histones wascarried out by G. Lo´pez-Rodas, Dept. of Biochemistry and Molecular ... fusion protein, the cDNAencoding p16 was obtained from the psp54 (28) cDNA. The oligonucleotides used as primers were: 5¢-CCCCTCGAGATGTCTAGACAAAAAAAGAGTAG-3¢ and 5¢-CCCCTCGAGTCACACAACAGCACGAC-3¢.ThePCRproduct ... loci and histones play a deﬁnite role in these changes, as they are the targets formany protein factors. The early view of histones as merestructural proteins changed about 10 years ago to envisagethem...

Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... functionalanalysis of glycosylation associated with diseases.Armed with our knowledge of human glycosylation,glycan structural analysis systems, the bioinformaticscapability and the databases that ... MS.AbbreviationsAAL, Aleuria aurantia lectin; AFP, a- fetoprotein; GGDB, GlycoGene Database; GlycoProtDB, GlycoProtein Database; GMDB, Glycan MassSpectral Database; HCC, hepatocellular carcinoma; HV, healthy ... Overlapping proteins appearing in the dark gray area of the Venn diagram are thought to be relatively abundant in serum, and are primary candidates for further validation. Glycoproteinsfound in the pale...

Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and 5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primersfor unmethylated DNA were: 5¢-GAAGTAGGTGGAGTATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCTACTT-3¢ (antisense).Caspase 3 activityCells ... (OccWT) and variant occludin inapoptosis and invasion, as determined by assay, werevealed that exon 9 played a major role in the induc-tion of mitochondria-mediated apoptosis and the reduction of ... expression of several apoptosis-related genes.Owing to the overexpression of OccWT, the expression of the apoptotic genes BAX and Apaf-1 increased, and that of the antiapoptotic gene Bcl-2 decreased....

Tài liệu Báo cáo khoa học: "A Finite-State Model of Human Sentence Processing" docx

... increasing am-biguity so that other ambiguity types also can bedisambiguated in a similar way via lexical cate-gory disambiguation. This idea has been exploredas one of the lexicalist approaches ... (1 3a) and (1 4a) compared to (13b) and (14b) at the disambiguating region that is bolded.(13) Different POS at the Disambiguating Region a. Katie laid the dress on the ﬂoor onto(−57.80) the ... this studyare parallel to empirical studies with regard to the design of experimental method and the test mate-rial. The garden-path sentences used in this studyare authentic, most of them are...

Tài liệu Báo cáo khoa học: "A semantically-derived subset of English for hardware verification" pdf

... 5). The horizontal axis of the diagram indicates the passing of time (as measured by clock cycles) and the vertical axis indicates the transition of signals between the states of high and ... CTL as (4). (4) AG('-,r AAXr ~ AF(- ,a AAX (a AAF(r AAX( ,r AAF (a AAX ,a) ))))) Example (3b) parallels (lb) in being closer to CTL than its (a) counterpart. Nevertheless, (3b) is ontologically ... build a prototype based on the Alvey Natural Language Tools Grammar (Grover et al., 1993). The Alvey grammar is a broad coverage grammar of English using GPSG-style rules, and maps into a event-based,...

Báo cáo khoa học: A Caenorhabditis elegans model of orotic aciduria reveals enlarged lysosome-related organelles in embryos lacking umps-1 function potx

... catalyzes the for-mation of phosphatidylinositol 3,5-bisphosphate and is orthologous to PIKfyve in mammals and Fab1p inyeast [21]. Cells lacking the function of these kinasesdisplay dramatically ... sequence and ﬂanking let-858 3¢-UTRwere ampliﬁed from a pvha-6p::GTWY::mCherry plasmid[80] using P568 5¢-ATGGTCTCAAAGGGTGAAGAAGATAAC-3¢ and P570 5¢-GGATTCTAGACTAGTTTTCCTTCCTCC-3¢. The cpr-6 and ... WHT-2 is localized to the gutgranule, where it mediates the transport of orotic acidfrom the cytoplasm to the gut granule. The swelling and vacuolization of lysosomes has longbeen known to result...

Báo cáo khoa học: "A Cost Sensitive Part-of-Speech Tagging: Differentiating Serious Errors from Minor Errors" pptx

... tag-ging. With the characteristics of a language (Rat-naparkhi, 1996; Kudo et al., 2004) and informa-tive features for POS tagging (Toutanova and Man-ning, 2000), the state -of- the- art supervised ... Proceedings of the Human Language Technology and North American Chapter of the Association forComputational Linguistics. pp. 252–259.Kristina Toutanova and Christopher D. Manning. 2000.Enriching the ... 9)Lexical featureswhether wicontains capitalswhether wihas a numberwhether wihas a hyphenwhether wi is all capitalwhether wistarts with capital and locates at the middle of sentenceTable...

Báo cáo khoa học: A client-binding site of Cdc37 ppt

... (Fig. 5A) . Alanine-scanning mutagenesis abolished the ability of mutant 3A to bind the Raf-1 kinase domain, and the ability of mutant 4A to bind the Raf-1 kinase domain wasremarkably decreased ... C10wtCα-FLAGWB: α-MycFLAG-GST wt N10 M10 C10 A 181 200wt 1A 2A 3A 4A 5A N10M10C10LVCEETANYLVIWCIDLEVEAAAAETANYLVIWCIDLEVELVCEAAAAYLVIWCIDLEVELVCEETANAAAAWCIDLEVELVCEETANYLVIAAAALEVELVCEETANYLVIWCIDAAAALVCEETANYLTANYLVIWCIVIWCIDLEVEFig. ... immunoprecipitation with anti-FLAG and anti-Myc monoclonal antibodies (IP: a- FLAG and a- Myc), followed by immunoblotting with anti-FLAG and anti-Mycpolyclonal antibodies (WB: a- FLAG and a- Myc). Asterisks...

Báo cáo khoa học: Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor pdf

... PI3-kinase/AKT and STAT3 activation pathways, LIFR/gp130signaling is also kn own to implic ate the GRAB2/Sosadaptators and regulate the MAP kinase pathway [54–56].ERK1 and ERK2, involved in the MAP ... Analysis of the downstream signaling events revealed the recruitmentby CC–FP of the signal transducer and activator of tran-scription (STAT)-3, Akt and mitoge n-activated protein (MAP) kinase ... 5326–5338.56. Takahashi-Tezuka, M., Yoshida, Y., Fukada, T., Ohtani, T.,Yamanaka, Y., Nishida, K., Nakajima, K., Hibi, M. & Hirano, T.(1998) Gab1 acts as an adapter molecule linking the cytokinereceptor...

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