Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx

... 5¢-CACTTG AAGCTTTAAGGAGGA AtagACCATGCGTATCCGTGAGCTTGGCATCACC-3¢; antisen se primer, 5¢-ACGCAA TCTAGAGTCAGCCCTCA GGGGGCTTTCG-3¢. The amplified PCR product was digested with HindIII and XbaI (Takara ... Experimen- tal procedures. Then a sample of the enzyme solution was taken, and the remaining activity of BapA was assayed with d-Ala-pNA as a substrate under standard condit...

Ngày tải lên: 07/03/2014, 21:20

... acetylated and nonacet- ylated isoforms and H2B was not acetylated at all. The acetylation and AUT/PAGE analysis of histones was carried out by G. Lo ´ pez-Rodas, Dept. of Biochemistry and Molecular ... fusion protein, the cDNA encoding p16 was obtained from the psp54 (28) cDNA. The oligonucleotides used as primers were: 5¢-CCCCTCGA GATGTCTAGACAAAAAAAGAGTAG-3¢ and 5¢-CC...

Ngày tải lên: 08/03/2014, 10:20

... functional analysis of glycosylation associated with diseases. Armed with our knowledge of human glycosylation, glycan structural analysis systems, the bioinformatics capability and the databases that ... MS. Abbreviations AAL, Aleuria aurantia lectin; AFP, a- fetoprotein; GGDB, GlycoGene Database; GlycoProtDB, GlycoProtein Database; GMDB, Glycan Mass Spectral Database; HCC, he...

Ngày tải lên: 16/02/2014, 08:20

... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and 5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT ACTT-3¢ (antisense). Caspase 3 activity Cells ... (Occ WT ) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon 9 played a major role in the induc- tion of mitocho...

Ngày tải lên: 18/02/2014, 18:20

... increasing am- biguity so that other ambiguity types also can be disambiguated in a similar way via lexical cate- gory disambiguation. This idea has been explored as one of the lexicalist approaches ... (1 3a) and (1 4a) compared to (13b) and (14b) at the disambiguating region that is bolded. (13) Different POS at the Disambiguating Region a. Katie laid the dress on th...

Ngày tải lên: 20/02/2014, 11:21

... 5). The horizontal axis of the diagram indicates the passing of time (as measured by clock cycles) and the vertical axis indicates the transition of signals between the states of high and ... CTL as (4). (4) AG('-,r AAXr ~ AF(- ,a AAX (a AAF(r AAX( ,r AAF (a AAX ,a) ))))) Example (3b) parallels (lb) in being closer to CTL than its (a) counterpart. Never...

Ngày tải lên: 20/02/2014, 19:20

... catalyzes the for- mation of phosphatidylinositol 3,5-bisphosphate and is orthologous to PIKfyve in mammals and Fab1p in yeast [21]. Cells lacking the function of these kinases display dramatically ... sequence and flanking let-858 3¢-UTR were amplified from a pvha-6p::GTWY::mCherry plasmid [80] using P568 5¢-ATGGTCTCAAAGGGTGAAGAAGA TAAC-3¢ and P570 5¢-GGATTCTAGACTAGTTTT...

Ngày tải lên: 06/03/2014, 09:22

... tag- ging. With the characteristics of a language (Rat- naparkhi, 1996; Kudo et al., 2004) and informa- tive features for POS tagging (Toutanova and Man- ning, 2000), the state -of- the- art supervised ... Proceedings of the Human Language Technology and North American Chapter of the Association for Computational Linguistics. pp. 252–259. Kristina Toutanova and Chri...

Ngày tải lên: 07/03/2014, 18:20

... (Fig. 5A) . Alanine- scanning mutagenesis abolished the ability of mutant 3A to bind the Raf-1 kinase domain, and the ability of mutant 4A to bind the Raf-1 kinase domain was remarkably decreased ... C10wt C α-FLAG WB: α-Myc FLAG-GST wt N10 M10 C10 A 181 200 wt 1A 2A 3A 4A 5A N10 M10 C10 LVCEETANYLVIWCIDLEVE AAAAETANYLVIWCIDLEVE LVCEAAAAYLVIWCIDLEVE LVCEETANAAAAWC...

Ngày tải lên: 07/03/2014, 21:20

... PI3- kinase/AKT and STAT3 activation pathways, LIFR/gp130 signaling is also kn own to implic ate the GRAB2/Sos adaptators and regulate the MAP kinase pathway [54–56]. ERK1 and ERK2, involved in the MAP ... Analysis of the downstream signaling events revealed the recruitment by CC–FP of the signal transducer and activator of tran- scription (STAT)-3, Akt and mit...

Ngày tải lên: 08/03/2014, 10:20