Báo cáo khoa học: Functional domains of the human epididymal protease inhibitor, eppin docx

... was observed with eppin or either of the domains (data not shown). Antibacterial activity of eppin and its domains The survival of E. coli XL-Blue cells exposed to eppin and its domains was assessed ... sequence. Eppin- 1 is expressed in the testis and epididymis, eppin- 2 is expressed in the epididymis only and eppin- 3 is expressed in the testis only [11]. Th...

Ngày tải lên: 23/03/2014, 07:20

... for future functional studies of Nog o-A. On the other hand, the recent identification of Nogo-40 as a potent NgR antagonist suggests a promising starting point for the design of potential therapeutic ... Zn 2+ . Furthermore, the d etermination of the Nogo-40 solution structure offers a starting point for further understanding the interaction between NgR and Nogo-40, and...

Ngày tải lên: 16/03/2014, 16:20

... with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala ... increase in the mutant mice, the disruption of DEC1 might be compensated by DEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The supp...

Ngày tải lên: 19/02/2014, 16:20

... centre of the junctions [19]. Detailed understanding of the functional role of these interactions would require the crystal structure of the Ydc2–junction complex. In this study the function of several ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pai...

Ngày tải lên: 20/02/2014, 11:20

... either component of the import machinery significantly decreased the amount of perox- ins of the importomer pulled down by the AAA perox- ins, whereas the amount of components of the AAA complex ... data not shown), thereby con- firming the specifity of the isolation. Taken together, these data indicate a close association of the mem- brane-bound AAA complex and...

Ngày tải lên: 07/03/2014, 12:20

... thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K mutants was found to be irreversible (Fig. S3). The propensity of the wild-type and each of the single ... introducing features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic prop- erties of the mutants, and s...

Ngày tải lên: 16/03/2014, 04:20

... active sites. Refolding of the scrambled RNase in the presence o f P DI was used as a positive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin from Alicyclobacillus ... wasusedasanegative control. The refolding of the scrambled RNase in the presence of Pf PDO seems to be less efficient when using PDI. However, t...

Ngày tải lên: 16/03/2014, 16:20

... amplitude of the fast phase of the l atter represen ted up to 40% of the total amplitude, with a rate constant (k obs ) independent of Pc concentration (data not shown). From the k obs values of this fast ... interactions with both PSI and Cyt f, but the solvent-exposed edge of His87, located in the hydrophobic patch at the so-called Ônorthern sideÕ of the protei...

Ngày tải lên: 16/03/2014, 16:20

... estimated from Hanes plots. Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... catalyse the synthesis and insertion of a cytidine dinucleotide cofactor into recombinant Qor as suggested by the release of CMP after hydrolysis of the enz...

Ngày tải lên: 17/03/2014, 10:20

... in the NdeI site of the pET15b vector (Novagen, Darmstadt, Germany). In this construct, the His6-tag sequence was fused to the N-terminus of the protein. The EVL fragments were expressed in the ... containing the SH3 and WW domains [17,24–26]. These facts suggest that the EVH1, Pro-rich and EVH2 domains all have the potential to function in protein binding. The p...

Ngày tải lên: 23/03/2014, 04:20