Báo cáo khoa học: Endosomal proteolysis of diphtheria toxin without toxin translocation into the cytosol of rat liver in vivo pdf

... FEBS Endosomal proteolysis of diphtheria toxin without toxin translocation into the cytosol of rat liver in vivo Tatiana El Hage 1,2 , Paulette Decottignies 3,4 and Franc¸ois Authier 1,2 1 INSERM, ... endosomes into the external milieu. Results In vivo endocytosis of DT and nontoxic mutant DT (mDT) within the endo-lysosomal apparatus of rat liv...

Ngày tải lên: 23/03/2014, 07:20

... be parsed, is the number of nonterminal categories in the grammar and is the number of (active) states in the FSA encoding of the grammar. The time bound is derived from counting the number of traversals ... the times in figure 3. The interesting cause of the varying exponents comes from the “constant” terms in the theoretical bound. The second half...

Ngày tải lên: 17/03/2014, 07:20

... may AB Fig. 5. The role of the C-terminal, HBXIP interacting, domain of hSuv3p in protein stability. (A) The protein stability of the wildtype hSuv3TAP and the hSuv3TAP variant lacking the C-terminal 136 ... cycloheximide to the culture medium. The protein levels were examined by Western blot in the indicated time points. (B) The protein stability of the fusi...

Ngày tải lên: 23/03/2014, 15:21

... be involved in signaling processes related to the regulation of synaptic plasticity by binding to the adaptor protein PSD95. This protein contains several PDZ domains one of which binds to the ... now allows to access even proteins that are present in rather few copies per cell including many of the hitherto functionally unassigned proteins. By putting these proteins into...

Ngày tải lên: 31/03/2014, 07:20

... B min þ ðB max À B min Þ 1 þ 10 ½ðlogIC 50 ÀLÞ n  ð1Þ where B is the maximal [ 3 H]daunomycin binding, B max is the maximal binding, B min is the minimum binding, IC 50 is the concentration of ... Figure 1B dem- onstrates the ability of the nucleotides to reduce the fraction of [ 3 H]daunomycin bound to ABCG2 R482G .In the presence of AMP, the binding of [ 3 H]d...

Ngày tải lên: 18/02/2014, 18:20

... N-terminal sequence of the 25- and 53-kDa fragments were the same as that of the whole recombinant wild-type protein indicating that the cleavage site was located at the C-terminus of each of these two ... single polypeptide chain containing 545 amino acids and consisting of two domains. The C-terminal glutamine amide transfer (GAT) domain catalyses the hydrolysis of...

Ngày tải lên: 23/03/2014, 17:22

... in loss of in vivo NirF activity. We demonstrate here that the product of the nirF gene is a periplasmic protein and, hence, must be involved in a late stage of the cofac- tor biosynthesis. In ... and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13 (Dnir...

Ngày tải lên: 15/02/2014, 01:20

... to insertion of the toxin in the membrane. The C domain is then translocated across the endosomal membrane into the cytosol. The C domain ADP-ribosylates the elongation factor 2, blocking protein translation ... chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-bindin...

Ngày tải lên: 16/02/2014, 09:20

... Seurin D, Segovia-Quinson B, Hardouin S & Binoux M (1986) Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding ... binding must only be a local effect because binding of PMP-BSA, which binds to other sites in the ectodomain of these heterodimers, resulted in the predicted amount of liga...

Ngày tải lên: 18/02/2014, 08:20

... were of two types, either with the two-domain architecture of the RibG protein of Bacil- lus subtilis or the RibD protein of E. coli, respectively [18–20], or the single-domain type of 2,5-diamino-6- ribitylamino-4(3H)-pyrimidinone ... reductase domain is located at the N-terminus and the predicted deaminase at the C-terminus of the bifunctional protein. In plant...

Ngày tải lên: 18/02/2014, 18:20