Báo cáo khoa học: The catalytic significance of the proposed active site residues in Plasmodium falciparum histoaspartic protease ppt

... histoaspartic protease FEBS Journal 275 (2008) 1698–1707 ª 2008 The Authors Journal compilation ª 2008 FEBS 1707 The catalytic significance of the proposed active site residues in Plasmodium falciparum histoaspartic ... interaction between the main chain oxygen of Ala216 and the e-amine of Lys78. In H34A mutant (Fig. 6B), the interaction between the...

Ngày tải lên: 23/03/2014, 07:20

... cleaves the protein at the C-terminal site of arginine and lysine, and the tryptic digest of FNR is predicted to contain the four N-terminal Cys residues in the peptide comprising amino acids ... form of air-inacti- vated FNR in vitro. Formation of apoFNR and the redox state of the cysteinyl residues were determined in vitro by alkylation. FNR contains five...

Ngày tải lên: 23/03/2014, 15:20

... and cofactor binding sites and important amino acid residues lining these pockets. The active site amino acid residues involved in substrate b inding were con served in Pf MDH but the N-terminal ... n the parasite. The brok en lines/arrows show inhibition a nd the double lines/ arrows indicate induction of the enzyme/enzymatic reaction in the presence of gossyp...

Ngày tải lên: 16/03/2014, 18:20

... presence of kringle 5 in the structure of the protease; miniplasmin (des-kringle 1-4 plasmin) is as sensitive to fatty acids as plasmin, whereas the activity of microplasmin (des-kringle 1-5 plasmin) ... of three models for the catalytic action of plas- min in the presence of stearate. The p-nitroaniline (P) released in the course of hydrolysis of 40 l M...

Ngày tải lên: 18/02/2014, 17:20

... compilation ª 2010 FEBS 2695 Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site Hiroaki Tsuji 1 , ... 2010) doi:10.1111/j.1742-4658.2010.07685.x The hyperthermostable chitinase from the hyperthermophilic archaeon Pyrococcus furiosus has a unique multidomain structure...

Ngày tải lên: 15/03/2014, 11:20

... N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the ribose are involved in coordinating a putative ... has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of the aspartic acid side chain position in HPRT...

Ngày tải lên: 15/02/2014, 01:20

... adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, which is implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated ... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3. In addition, f...

Ngày tải lên: 16/02/2014, 09:20

... strains [3]. Pathogenic Yersiniae are typically taken up through ingestion and first reach the intestine. The Yersinia surface protein invasin binds to b1 integrins on the apical surface of M ... In addition to actin, other host cell proteins have been shown to bind to the virulence factor YpkA, including OTUB1 [13]. In order to investigate the role of OTUB1 in Yersinia inv...

Ngày tải lên: 16/02/2014, 15:20

... number of these modifications is low, the density of modifications is much the same as in the rest of the lin- ker histones. It is the shortness of the N-terminal tails Table 1. Alignment of chicken, ... the triphosphate form being incorpo- rated into DNA in place of the natural base cytosine [12]. The use of such analogues results in the global depletion...

Ngày tải lên: 18/02/2014, 08:20

... focusing on the molecular mechanisms by which SREBPs are involved in lipotoxicity. SREBPs directly repress the transcription of insulin receptor substrate-2 (IRS-2), the main insulin signaling ... apparatus, where the site 1 and site 2 proteases cleave the SREBPs [3,4]. Upon restoration of cellular cholesterol, Insig, another key regulator of ER membrane proteins, traps...

Ngày tải lên: 18/02/2014, 13:20