Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx

... (PGEL in human), split into the variable posi-tions 1 and 2, and the conserved EL ⁄ DL in positions 3 and 4. Species with canonical retention signals are colored in blue, and noncanonical signals in ... noncanonical retention signals S. L. Gande et al.1130 FEBS Journal 275 (2008) 111 8–1 130 ª 2008 The Authors Journal compilation ª 2008 FEBS Paralog of the formylglycine-generating enzyme – retention in the ... pFGE by western blotting, usingan antiserum against pFGE. The total expression level and the per-centage of intracellular pFGE are given.ER retention by noncanonical retention signals S. L. Gande...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... helix–span–helix domain at the C-terminus shown in blue medi-ates dimerization and, together with the basic domain, (red) DNA-binding. ‘*’, amino acids that are identical in all sequences in the ... helix–span–helix DNA-binding and dimerization domain at their C-terminus, and a lessconserved proline-rich and glutamine-rich transactiva-tion domain at their N-terminus [ 8–1 0]. Most isoformsalso have ... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen-tiation stages during chondrogenesis and the involvement of transcription factors(henatoxylin and eosin-stained section of...

Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

... receptor-blockingexperiments. The rates of uptake of both the lipid and protein components are not matched by the rate of cel-lular metabolism of these species, resulting in the accu-mulation of both unmodiﬁed ... and glycated apoB in the cells. The rate of removal of the latter species is slow, with only 2 0–3 0% of the glycatedprotein being degraded over a 24 h chase period. In contrast to the rapid and ... receptorsSR-A and CD36. The accumulation of lipid in thesemacrophages is accompanied by increased endocytosis and degradation of apoB, with the difference in the rates of the latter two processes resulting...

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... 17.6Fig. 5. Insertion of steﬁns into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into the sub-phase ... signal increase during the injec-tion phase was the largest and there was low dissoci-ation after the end of injection.Discussion The main hypothesis for pathology in AD and otherneurodegenerative ... [17,18]. By following the kinet-ics of ﬁbril formation, conditions were deﬁned in which the protein exists in the form of preﬁbrillaroligomers ⁄ aggregates, which persist during the lagphase. These...

Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

... similar position as the lowest faint bandobserved for wild-type Kv3.1 and the lower faint band of the single mutants, and furthermore, the immuno-band did not shift in the presence of TM. To verifythat ... utilized in rat brain and infectedSf9 cells, and the role that occupancy of these siteshas in the expression of functional Kv3.1s at the cellsurface of Sf9 cells. Immunoband patterns of wild-type ... faint bands of wild-type Kv3.1 or the lowestfaint band, respectively. These results indicate that the most prominent band of wild-type Kv3.1 expressed in insect cells represents occupancy of...

Báo cáo khoa học: Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling pdf

... receptors, including human RXR (which is the ortholog of USP) have shown that an area on the surface of the ligand binding domain involving the C-terminus of a-helix 3, a-helix 4, and the N-terminus of ... activation by JH III by way of binding of JH IIIor closely related structures to the ligand bindingpocket of USP [18]. This binding of JH III by USPinduces change in USP tertiary conformation [18] and induces ... both the dEcR and USP in nuclear extracts are in the complexthat binds to the DR1 motif in EMSA, and this over-expression of EcR increases the activation of DR1JHECore by 20OHE. We infer from these...

Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt

... shown). The basic cluster of amino acids in the bindinggroove of 14-3-3, including amino acids Lys-49, Arg-56, Lys-120 and Arg-127, in an otherwise acidic mole-cule, are important for the interaction ... Petri dishes and incubated with medium contain-ing penicillin, streptomycin and gentamicin to ascertain the reversibility of this cytotoxic response (E–H).L. Yasmin et al. Delineation of ExoS residuesFEBS ... forphosphorylation-independent binding The interaction between 14-3-3 and ExoS is importantfor the ADP ribosylation activity of ExoS and evenmore intriguingly, appears to be independent of serine-phosphorylation...

Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx

... traces of animals in the ﬁrst two weeks of lactation. The fractioncontaining eugenin is indicated by an asterisk in Fig. 2.Two further HPLC separations of the initial eugenin frac-tion (1 0–7 5% ... asstimulation of the proliferation of lymphocytes, activa-tion of CCK receptors stimulate production of inter-leukins and secretion of immunoglobulins on the mucosal surface of the intestine [49,50]. The ... euge-nin, indicating that eugenin is indeed acting throughCCK2receptors.To further investigate the interaction of eugenin withCCK2receptors, we investigated the effect of eugeninon lymphocyte...

Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx

... DvHc3causing narrower NMR signals and perturbing the chemical shifts of the haem methyl sig-nals in intermediate redox stages. The contraction of the line widths of the NMR signals in intermediateredox ... obtained forDvHc3:ZnRb and DvHc3is also reported in Fig. 4 and indicated by continuous and dashed lines, respectively. The ﬁgure shows that the effect of binding Zn-rubre-doxin on the NMR signals ... essentiallyundisturbed by the replacement of Fe by Zn in the rubredoxin given the identical structures of the twoprotein forms [38,39]. Therefore, at the concentrationsused in the NMR experiments over 90% of the cytochrome...

Tài liệu Báo cáo khoa học: "Using adaptor grammars to identify synergies in the unsupervised acquisition of linguistic structure" docx

... We initialize the sampler by randomly as-signing each string in the training corpus a randomtree generated by the grammar. Then we randomlyselect a string to resample, and sample a parse of ... grammar, in which the subtrees are speciﬁed in advance, in an adaptor grammar the subtrees, aswell as their probabilities, are learnt from the train-ing data. In order to make parsing and inferencetractable ... terminal in the grammar. If Ais a terminal then GAis the distribution that puts all of its mass on the unit tree (i.e., tree consisting of asingle node) labeled A. If A is a nonterminal thenGAis...

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