Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt

... role in maintaining the normal activity of mitochondrial FAD- binding enzymes, including lipoamide dehydrogenase and succinate dehydrogenase flavoprotein subunit Sdh1p. A decrease in the amount of ... 1117 Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transpor...

Ngày tải lên: 23/03/2014, 07:20

... decreasing the activity of caspase-3 in the three cell lines. Effect of HSS knockdown on CCCP-induced apoptosis To further elucidate the functional role of HSS in CCCP-induced apoptosis, the expression ... and compromises the normal integrity of the mitochondrial IM. This results in the IM becoming freely permeable to protons, leading to the uncoupling of o...

Ngày tải lên: 16/02/2014, 09:20

... type of interac- A B 347150381203 442 BTBHTAMBPM1 189 1–1 51 F RAP2.4 1 150 214 AP2 334 60 12 5–2 51 C D BPM1 BPM1 1–1 51 BPM1 1–1 89 GST GST:RAP2.4 – – – – ++ + – – –+ – – + BPM1 T7 input BPM1 1–1 51 BPM1 1–1 89 –+ – +– – + –+ GST GST:BPM1 T7 ... input BPM1 1–1 51 BPM1 1–1 89 –+ – +– – + –+ GST GST:BPM1 T7 input...

Ngày tải lên: 18/02/2014, 06:20

... addition of PHI, the fluorescence of PHR-bound flavin increases. This finding points to a PHI-induced conformational change of PHR, possibly resulting in a more pronounced exposure of FAD to the aqueous ... reaching thermal equilibrium. As shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide b...

Ngày tải lên: 21/02/2014, 00:20

... corresponded closely to the transition midpoint of abolition of the kinetic lag. In the case of inactivation by trypsin, the profile distinctly indicated the existence of a stable intermediate between 0.1 ... (lower) domains of a monomer; the rectangular denting of the upper domains of each lobe indicates the binding site of 5¢-UMP ex vivo; the shaded rectan...

Ngày tải lên: 16/03/2014, 00:20

... subdivided into two main groups (i.e. the quinoxalines and the quinolines), depending on the chromophore moiety bound to the N-termini of each oligopeptide chain. Prominent mem- bers of the quinoxaline-group ... assembly line [11]. The resulting structure can be either branched-cyclic, as in the case of the last-line antibiotic daptomycin, or closed, as in the case...

Ngày tải lên: 23/03/2014, 06:20

... in the same range as that of the recombinant protein [19]. Interestingly the specific activity of the recombinant P. falciparum ICDH is about 4 fold higher than that reported for the porcine mitochondrial ... be conserved in P. falciparum ICDH. In porcine ICDH the positive charges of the arginine residues Arg101, Arg110 and Arg133 are responsible for the binding...

Ngày tải lên: 08/03/2014, 02:20

... found in the NAD + -binding site in ADHs [34]. The absence of the probable initiator methionine in the native enzyme may be due to the N-terminal processing commonly observed in eukaryotic proteins ... 154, 19 3–1 96. 36 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram qualities of protein utiliz- ing the principle of protein-dy...

Ngày tải lên: 16/03/2014, 06:20

... significantly in the presence or absence of the b 1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b 1 subunit. The effects of b 1B were further assessed ... Na V 1.2. The increase in ionic currents induced by coexpression of the human b 1B could result from an increase in the number of chann...

Ngày tải lên: 16/03/2014, 23:20

... promoting an increase in proopiomelanocortin and cocaine and amphetamine regulated transcript. Taken together, these findings provide a compelling argument for the role for malo- nyl-CoA in regulating ... and certain amino acids [16]. A linkage between fatty acid synthesis in the CNS and feeding behavior was uncovered with the finding that systemic or intracerebroventricular adminis...

Ngày tải lên: 14/02/2014, 22:20