Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx

... ¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis, in vitro transfection activity, and physicochemical characterization Michael Spelios and Michalakis Savva Division of Pharmaceutical ... PL (1994) Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations. J Biol Chem 269 ,...

Ngày tải lên: 23/03/2014, 07:20

... ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss the possibility that both could func- tion as molecular targets for the prevention and treatment of obesity and metabolic ... focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes- ity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and...

Ngày tải lên: 14/02/2014, 22:20

... The K m value for NADH was determined in the pre- sence of FNR (200 n M) and NADH (0. 5–7 .0 mM), and the K m value for NADPH was determined in the presence of FNR (20 n M) and NADPH ( 2–1 00 l M). NADH ... vector, and the construct was designated pET–FNR. E. coli BL21(DE3) Codon Plus cells were transformed with pET–FNR. The transformant was grown in 2 · YT medium containing c...

Ngày tải lên: 18/02/2014, 08:20

... template for a second cycle of mutagenesis and selection (10 000 mutants). It afforded 351 novel candidate strains. After rescreening, 10 mutant proteins were purified and characterized, and their ... were supplied by Fluka (Buchs, Switzerland). LB (Luria–Bertani) medium was from Becton Dickinson (Basel, Switzerland). Generation of mutant libraries For the generation of the ribA muta...

Ngày tải lên: 18/02/2014, 11:20

... attributed to mutations in helicase genes [ 1–4 ]. Helicases act on double-stranded nucleic acid substrate and thus can be designated DNA–DNA, RNA–DNA or RNA–RNA helicases depending on the composition ... been suggested for a number of the conserved motifs [1 0– 12]. For example, motif I (A ⁄ GxxGxGKT), motif II (VLDEAD), motif III (SAT) and motif VI (HRIGRxxR) are responsible f...

Ngày tải lên: 18/02/2014, 16:20

... 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former, and LTA–M2F, 5¢-CAGCTGTTTTGCTTGAATTATG-3¢ and LTA–2R, 5¢-GAATTCATTATGTTTCAGGTTCA GGGG-3¢ for the latter. The PCR products were cloned into the EcoRV site of pZErO-2 (Invitrogen). ... 200 lm. (B) Prox–1 and VEGFR–3 were also expressed in Lyve–1-positive ECs, confirming that they maintained lymphatic endothelial properties. (C) VEGF–A and VEG...

Ngày tải lên: 18/02/2014, 17:20

... bonded pairs have different C a and C b distances than hydrogen bonded pairs and therefore may have different residue preferences and potential for side chain–side chain interactions. Initial ... varied (for instance, core residues 3 and 39, and boundary residues 25 and 29 that were all changed in a hyperstable GB1 variant [12]). Based on the computational studies, core positio...

Ngày tải lên: 19/02/2014, 12:20

... Association for Computational Linguistics, pages 38 3–3 87, Jeju, Republic of Korea, 8-14 July 2012. c 2012 Association for Computational Linguistics Unsupervized Word Segmentation: the case for Mandarin ... II (Emerson, 2005) and define a more precise topline for the task using cross-trained supervized system available off-the-shelf (Zhang and Clark, 2010; Zhao and Kit, 2008;...

Ngày tải lên: 19/02/2014, 19:20

... 77 1–7 77. Stem–loop padlock oligonucleotides for dsDNA B. Ge ´ ron-Landre et al. 5352 FEBS Journal 272 (2005) 534 3–5 352 ª 2005 FEBS Stem–loop oligonucleotides as tools for labelling double-stranded ... TÆAxT and CÆGxG triplets with an antiparallel TG TFO. Stem–loop padlock oligonucleotides for dsDNA B. Ge ´ ron-Landre et al. 5344 FEBS Journal 272 (2005) 534 3–5 352 ª 2005 FEB...

Ngày tải lên: 20/02/2014, 03:20

... the in-domain gold standard classification result. For example, the in-domain goal standard for the Book domain is 79.96%. For adapting from DVD to Book, baseline achieves 72.25% and JST gives 76.45%. The ... 257859). References R.K. Ando and T. Zhang. 2005. A framework for learn- ing predictive structures from multiple tasks and un- labeled data. The Journal of Machine Learning...

Ngày tải lên: 20/02/2014, 04:20