Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf
... 138 –147 ª 2007 The Authors Journal compilation ª 2007 FEBS 147
Human retinol dehydrogenase 13 (RDH13) is a
mitochondrial short-chain dehydrogenase
⁄
reductase
with a retinaldehyde reductase activity
Olga ... 4).
Activity assays showed that purified RDH13–His6 was
indeed active towards all-trans -retinaldehyde and
appeared to prefer NADPH to NADH as a co...
... Molecular Graphics
System. DeLano Scientific, San Carlos, CA, USA.
41 Wallace AC, Laskowski RA & Thornton JM (1995)
LIGPLOT: a program to generate schematic diagrams
of protein–ligand interactions. ... GluR -A and of two
variant PDZ2 domains in unliganded state at 1.8–2.44 A
˚
resolutions.
SAP97
PDZ2
folds to a compact globular domain comprising six b-strands
and two a- helices, a...
... maintenance, and is essential for chromo-
somal DNA replication. Proc Natl Acad Sci USA 93,
12309–12314.
13 Mimura S, Masuda T, Matsui T & Takisawa H (2000)
Central role for cdc45 in establishing ... antibody on formalin-fixed and
paraffin-embedded tissues (Fig. 9). Antibodies against
PCNA and Cdc45 stained malignant cells in a compar-
able manner, e.g. on invasive-lobular mamma carci...
... QuickChange site-directed mutagenesis kit
(Stratagene, La Jolla, CA, USA): C153S-up, 5¢-TGCATC
TTGCACTACCAACTCTCTTGCTCCCTTTGTC-3¢; and
C153S-down, 5¢-TGACAAAGGGAG CAAGA GAGTTGG
TAGTGCAAGATGC-3¢. ... triangles). NADPH-dependent activity is given as a
percentage of the initial activity. (B) Reversal of A
4
-GAPDH inactiva-
tion by dithiothreitol. A
4
-GAPDH was inactivated by incubat...
... Journal 272 (2005) 2869–2877 ª 2005 FEBS 2875
5¢-TCAGCGTTCTCGGAATTC-3¢; AP2-Xba-R, 5¢-TTTT
ACAGTAATATAAAGAATTTCGCTCTAGATCAAGGA
CCTCCCGCAAGCGCGAG-3¢; and AP2-R, 5¢-TTACA
GTAATATAAAGAATTTCGCTCTAGA-3¢, ... MgCl
2
using an ALS
Electrochemical Analyzer 62 4A, and the potential was deter-
mined by averaging the anodic and cathodic peak potentials.
Presteady-state kinetic studies
Presteady-state red...
... potential
as a large-scale source of human IF for analytical and
therapeutic purposes.
Keywords: arabidopsis; cobalamin; intrinsic factor; recom-
binant.
Vitamin B
12
(cobalamin, Cbl) is the ... signal peptide (Ext)
with the amino acid sequence MASSSIALFLALNL
LFFTTISA and 47 nucleotides from the 5¢-untranslated
region. This sequence is part of the plant A. thaliana
cDNA sequence in...
... Hill Road
Palo Alto, CA 94304, USA
mforst@parc.com
Abstract
In this paper we present a human- based
evaluation of surface realisation alterna-
tives. We examine the relative rankings of
naturally ... European Chapter of the ACL, pages 112–120,
Athens, Greece, 30 March – 3 April 2009.
c
2009 Association for Computational Linguistics
Human Evaluation of a German Surface Realisation Rank...