Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx

... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers. All of the bands stained positively ... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig. 1, lane 1). However, in the absence of boiling (...

Ngày tải lên: 21/02/2014, 00:20

... target cells and the fate of its two subunits, RTA and the cell-binding galactose-specific lectin ricin toxin B chain (RTB), are essential to gain further insights into the mechanism of toxin action ... CP133 5¢-TTAAAACTGTGACGATGGT GGA-3¢ with the TAA termination anticodon shown in bold. Amplification reactions were performed in a final vol- ume of 50 lL containing 5 ng of...

Ngày tải lên: 23/03/2014, 07:20

... 0.81 ATcAAACA 0.03 c ATtAAACA 0.01 c ATGcAACA 0.69 ATGgAACA 0.20 c I ATGAgACA 0.02 c ATGAAgCA 0.05 c ATGAAAgA 0.30 III ATGAAACg 0.26 a PREs represented in the FUS1 promoter (Fig. 4B). b RCS for each oligo ... significant Table 1. RCS of mutant PREs for binding of wild-type Ste12 to a PRE consensus (ATGAAACA) in vitro. FUS1 PRE a Sequence RCS b II ATGAAACA 1.00 IV tTGAAACA 0.27 c...

Ngày tải lên: 06/03/2014, 22:21

... GTCGTAATTAATCACTTAACCGTGCTCG CYP71 9A2 reverse GAAAGAAACAGAGCAAATCTTATCCTTTTACC CYP71 9A3 forward CCTCGTAACTAATATACCAGTGTGGTG CYP71 9A3 reverse GACAACCAAGCAAACTCTTATTCTTGTAC Internal control gene b-actin ... reverse CAATGGAGTTGGTGGGTGAA BBE forward GAGATTAGTAGGAGTTGGGGTGAGA BBE reverse ATTGGAGGGATACTTTGTGGATG 4’-OMT forward CCTAGAAGAGGAATCAGAACATCCA 4’-OMT reverse TCACTTCTCTCCCTTCCACCA CYP71...

Ngày tải lên: 07/03/2014, 10:20

... 5¢-RACE Zf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACE Zf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACE Zf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC 3¢-RACE Zftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCR Zftbet-R1 CACTGGATGAGACAGGAAGTT ... GGAAACTTCCTGTCTCATCCAGTG 5¢-RACE Zffoxp3-F1 GGAACACACAGAGGGGATGATA Initial PCR Zffoxp3-R1 CTTCAACACGCACAAAGCAC Initial PCR Zffoxp3-F2 TGCCACCTTTTCCATCATACA Initial PCR Zffox...

Ngày tải lên: 16/02/2014, 09:20

... Bovine submaxillary mucin, which contains mainly 9-O-acetyl- and 8,9 di-O-acety-N- acetyl neuraminic acid was the most potent inhibitor of the lectin. Sialidase treatment and de-O-acetylation of ... lectin on SDS/PAGE gave a single band at apparent molecular mass of 34 kDa. The binding affinity of the lectin in the hemolymph of the freshwater crab, Paratelphusa jacquemontii,...

Ngày tải lên: 21/02/2014, 00:20

... members, including AF499, have an N-terminal Ôtwin-arginineÕ signal sequence that is characteristic of cofactor-containing proteins translocated into the periplasm via the Tat translocase [27]. As ... with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal sequences of the polypeptides of the purified enzyme....

Ngày tải lên: 21/02/2014, 03:20

... using a sense primer, 5¢-CATATGAATGCTCTCATCTG CGAGG ACA-3¢, containing a 5¢ NdeI site and start methionine resi- due, and an antisense primer, 5¢-GGATCCTAGTGATG GTGATGGTGGCATCCATACAGCTTATTGATCC-3¢, which ... forward: 5¢-CTGAACT TCTCTACACACTGAGG-3¢, reverse: 5¢-CCTTATCACC ATCACCTCACTTC-3¢; ZHE2 forward: 5¢-CTCCACACA CTGAGACTAAATGG-3¢, reverse: 5¢-GGAAATAAGAG CACGTACTGTGG-3¢. The cycle...

Ngày tải lên: 07/03/2014, 04:20

... gene assay in in vivo results. Experimental procedures Parasites The NMRI strain of S. mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesocricetus auratus ... motifs binding to the cis-regulatory region of a target gene, and a conserved ligand-binding domain (LBD) that is involved in transcriptional activation of the target gene via li...

Ngày tải lên: 07/03/2014, 11:20

... proteinases it can be deduced that the common ancestor of the genera Carica and Vasconcellea already contained at least two different cysteine pro- teinases in its latex (papain and chymopapain). ... translation showed that they hold the characteristic features of all known papain-class cysteine proteinases, and a phylogenetic analysis revealed the existence of several papain...

Ngày tải lên: 07/03/2014, 11:20